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Interaction of Bone Marrow Stromal Stem Cells with Adhesive Protein and Polypeptide-adsorbed Poly(lactide-co-glycolide) Scaffolds  

Choi, Jin-San (Department of Advanced Organic Materials Engineering, Chonbuk National University)
Lee, Sang-Jin (Department of Chemical Engineering, Hanyang University)
Jang, Ji-Wook (Department of Polymer Engineering, Pukyong National University)
Khang, Gil-Son (Department of Advanced Organic Materials Engineering, Chonbuk National University)
Lee, Young-Moo (Department of Chemical Engineering, Hanyang University)
Lee, Bong (Department of Polymer Engineering, Pukyong National University)
Lee, Hai-Bang (Biomaterials Laboratory, Korea Research Institute of Chemical Technology)
Publication Information
Polymer(Korea) / v.27, no.5, 2003 , pp. 397-404 More about this Journal
Abstract
The interaction of cell adhesive protein and polypeptide with bone marrow stromal stem cells (BMSCs) grown in tissue engineered films and scaffolds were examined. Several proteins or polypeptide known as cell-adhesive were coated adsorption on poly(lactide-co-glycolide) (PLGA) films and scaffolds and adhesion and proliferation behavior of BMSC on those surfaces were compared. The protein and polypeptide used include collagen IV, fibrinogen, laminin, gelatin, fibronectin, and poly(L-lysine). The protein and polypeptide were adsorbed on the PLGA film surfaces with almost monolayer coverage except poly(L-lysine). BMSCs were cultured for 1, 2, and 4 days on the protein- or polypeptide-adsorbed PLGA films and scaffolds. The cell adhesion and proliferation behaviors were assessed by sulforho damine B assay. It was observed that the protein- or polypeptide-adsorbed surfaces showed better cell adhesion and proliferation than the control.
Keywords
cell adhesive biomolecules; poly(lactide-co-glycolide); bone marrow stromal stem cell; cell interaction.;
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