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Comparison of Extraction Procedures for the Determination of Capsaicinoids in Peppers  

Jeon, Geonuk (Department of Food Science and Technology, Chungbuk National University)
Lee, Jun-Soo (Department of Food Science and Technology, Chungbuk National University)
Publication Information
Food Science and Biotechnology / v.18, no.6, 2009 , pp. 1515-1518 More about this Journal
Abstract
The objective of this study was to compare 3 extraction methods including, solid phase extraction (SPE), acetonitrile extraction, and methanol extraction, for their usefulness as extraction methods to determine capsaicinoids. The determination of capsaicinoids in the extracts was carried out on a reverse-phased high performance liquid chromatography (HPLC) using a fluorescence detector. Three extraction methods, i.e., SPE, acetonitrile extraction, and methanol extraction were compared for the quantification of capsaicinoids using raw peppers and pepper powder. The highest analytical values were observed using methanol extraction and the lowest values using SPE. Also, the analytical method validation parameters such as accuracy, precision, limit of detection, limit of quantitation, and specificity were calculated to ensure the method's validity. This method provides a fast and accurate approach for the determination of capsaicinoids in peppers.
Keywords
capsaicinoid; pepper; extraction method; high performance liquid chromatography (HPLC);
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