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Enhancement of Anti-tumorigenic Polysaccharide Production, Adhesion, and Branch Formation of Bifidobacterium bifidum BGN4 by Phytic Acid  

Ku, Seock-Mo (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University)
You, Hyun-Ju (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University)
Ji, Geun-Eog (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University)
Publication Information
Food Science and Biotechnology / v.18, no.3, 2009 , pp. 749-754 More about this Journal
Abstract
The polysaccharide (BB-pol) extracted from Bifidobacterium bifidum BGN4 showed growth inhibitory effects on several colon cancer cell lines such as HT-29 and HCT-116. To increase the yield of polysaccharide, B. bifidum BGN4 was cultured in various culture media with different compositions. When B. bifidum BGN4 was cultured in modified MRS broth containing phytic acid, the cells showed increased branch formation and enlarged morphology. The content of total carbohydrate and the ability of adhesion to intestinal epithelial cells were also increased by phytic acid. The polysaccharide obtained from the cells grown in the presence of phytic acid inhibited the proliferation of cancer cell lines such as HT-29 and MCF-7 cells but not normal colon cell line, FHC. Taken together, Bifidobacterium grown in the presence of phytic acid may confer enhanced beneficial function for the host.
Keywords
Bifidobacterium; polysaccharide; anti-tumorigenicity; phytic acid; cell adhesion;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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1 O'Mahony L, Feeney M, O'Halloran S, Murphy L, Kiely B, Fitzgibbon J, Lee G, O'Sullivan G, Shanahan F, Collins JK. Probiotic impact on microbial flora, inflammation and tumor development in IL-10 knockout mice. Aliment. Pharm. Therap. 15: 1219-1225 (2001)   DOI   ScienceOn
2 Gaudier E, Michel C, Segain JP, Cherbut C, Hoebler C. The VSL#3 probiotic mixture modifies microflora but does not heal chronic dextran sodium sulfate–induced colitis or reinforce the mucus barrier in mice. J. Nutr. 135: 2753-2761 (2005)   DOI
3 Parvez S, Malik KA, Kang SA, Kim HY. Probiotics and their fermented food products are beneficial for health. J. Appl. Microbiol. 100: 1171-1185 (2006)   DOI   ScienceOn
4 Salminen S, Bouley MC, Boutron-Rualt MC, Cummings J, Franck A, Gibson G, Isolauri E, Moreau MC, Roberfroid M, Rowland I. Functional food science and gastrointestinal physiology and function. Brit. J. Nutr. 1: S147-S171 (1998)   DOI   ScienceOn
5 Kim JY, Seo HS, Seo JM, Suh JW, Hwang IK, Ji GE. Development of S-adenosyl-L-methionine (SAM) reinforced probiotic yogurt using Bifidobacterium bifidum BGN4. Food Sci. Biotechnol. 17: 1025-1031 (2008)   과학기술학회마을   ScienceOn
6 Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350-356 (1956)   DOI
7 Perez PF, Minnaard Y, Disalvo EA, DeAntoni GL. Surface properties of bifidobacterial strains of human origin. Appl. Environ. Microb. 64: 21-26 (1989)   ScienceOn
8 Kim IH, Park MS, Ji GE. Characterization of adhesion of Bifidobacterium sp. BGN4 to human enterocyte-like Caco-2 cells. J. Microbiol. Biotechn. 13: 276-281 (2003)   과학기술학회마을   ScienceOn
9 Servin AL, Coconnier MH. Adhesion of probiotic strains to the intestinal mucosa and interaction with pathogens. Best Pract. Res. Cl. Ga. 17: 741-754 (2003)   DOI   PUBMED   ScienceOn
10 Hudault S, Lievin V, Bernet-Camard MF, Servin AL. Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection. Appl. Environ. Microb. 63: 513-518 (1997)   PUBMED   ScienceOn
11 Kim JY, Suh JW, Ji GE. Evaluation of S-adenosyl-L-methionine (SAM) production by Bifidobacterium bifidum BGN4. Food Sci. Biotechnol. 17: 184-187 (2008)   과학기술학회마을   ScienceOn
12 Fuller R. Probiotics in man and animal. J. Appl. Bacteriol. 66: 365-378 (1989)   DOI
13 Kojima M, Suda S, Hotta S, Hamada K. Induction of pleomorphism in Lactobacillus bifidus. J. Bacteriol. 95: 710-711 (1968)   PUBMED
14 Oda M, Hasegawa H, Komatsu S, Kambe M, Tsuchiya F. Antitumor polysaccharide from Lactobacillus sp. Agr. Biol. Chem. 47: 1623-1625 (1983)   DOI
15 Bernet MF, Brassart D, Neeser JR, Servin AL. Lactobacillus acidophilus LA1 binds to cultured human intestinal cell lines and inhibits cell-attachment and cell invasion by enterovirulent bacteria. Gut 35: 483-489 (1994)   DOI   ScienceOn
16 Wadstrom T, Andersson K, Sydow M, Axelsson L, Lindgren S, Gullmar B. Surface properties of lactobacilli isolated from the small intestine of pigs. J. Appl. Bacteriol. 62: 513-520 (1987)   DOI   ScienceOn
17 de Vrese M, Schrezenmeir J. Probiotics, prebiotics, and synbiotics. Adv. Biochem. Eng. Biot. 111: 1-66 (2008)   DOI   PUBMED   ScienceOn
18 Sekine K, Ohta J, Onishi M, Tatsuki T, Shimokawa Y, Toida T, Kawashima T Hashimoto Y. Analysis of antitumor properties of effector cells stimulated with a cell wall preparation (WPG) of Bifidobacterium infantis. Biol. Pharm. Bull. 18: 148-153 (1995)   DOI   PUBMED   ScienceOn
19 You HJ, Oh DK, Ji GE. Anticancerogenic effect of a novel chiroinositol-containing polysaccharide from Bifidobacterium bifidum BGN4. FEMS Microbiol. Lett. 240: 131-136 (2004)   DOI   ScienceOn
20 Kojima M, Suda S, Hotta S, Hamada K. Induction of pleomorphy and calcium ion deficiency in Lactobacillus bifidus. J. Bacteriol. 102: 217-220 (1970)   PUBMED
21 Ofek I, Doyle RJ. Bacterial Adhesion to Animal Cells and Tissues. Chapman & Hall, New York, NY, USA. pp. 158-177 (1994)
22 Kojima M, Suda S, Hotta S, Hamada K. Suganuma A. Necessity of calcium ion for cell division in Lactobacillus bifidus. J. Bacteriol. 104: 1010-1013 (1970)