Browse > Article

Effects of Aucubin Isolated from Eucommia ulmoides on UVB-induced Oxidative Stress in Human Keratinocytes HaCaT  

Ho, Jin-Nyoung (Department of Food and Biotechnology, Han-Seo University)
Cho, Hong-Yon (Department of Food and Biotechnology, Korea University)
Lim, Eun-Jeong (Department of Food and Nutrition, Hanyang Women's College)
Kim, Hye-Kyung (Department of Food and Biotechnology, Han-Seo University)
Publication Information
Food Science and Biotechnology / v.18, no.2, 2009 , pp. 475-480 More about this Journal
Abstract
Ultraviolet B (UVB) radiation provokes the generation of reactive oxygen species (ROS) in the cells and skin, which induce oxidative stress in the exposed cells, leading to photoaging and cancer. Using the human keratinocytes HaCaT cell line, we investigated the photoprotective effects of aucubin isolated from Eucommia ulmoides. Pretreatment with aucubin markedly suppressed UVB-induced oxidative stress, which manifests as a decrease in intracellular lipid peroxidation, elevation of catalase activity, and reduced glutathione content. In addition, aucubin significantly reduced expression of matrix metalloproteinase-1 (MMP-1) protein (54%) and mRNA. Taken together, these results suggest that aucubin may offer protection against UVB-induced oxidative stress and may be used as a potential agent in prevention of UVB-induced photoaging.
Keywords
photoaging; ultraviolet B (UVB); aucubin; oxidative stress; matrix metalloproteinase-1 (MMP-1);
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Psotova J, Svobodova A, Kolarova H, Walterova D. Photoprotective properties of Prunella vulgaris and rosmarinic acid on human keratinocytes. J. Photoch. Photobio. B 84: 167-174 (2006)   DOI   ScienceOn
2 Erden IM, Kahramant A, Kokent T. Beneficial effects of quercetin on oxidative stress induced by ultraviolet A. Clin. Exp. Dermatol. 26: 536-539 (2001)   DOI   ScienceOn
3 Steinbrenner H, Ramos MC, Stuhlmann D, Sies H, Brenneisen P. UVA-mediated downregulation of MMP-2 and MMP-9 in human epidermal keratinocytes. Biochem. Bioph. Res. Co. 308: 486-491 (2003)   DOI   ScienceOn
4 Yen GC, Hsieh CL. Reactive oxygen species scavenging activity of Du-Zhong (Eucommia ulmoides Oliv.) and its active compounds. J. Agr. Food Chem. 48: 3431-3436 (2000)   DOI   ScienceOn
5 Fischer TW, Zbytek B, Sayre RM, Apostolov EO, Basnakian AG, Sweatman TW, Wortsman J, Elsner P, Slominski A. Melatonin increases survival of HaCaT keratinocytes by suppressing UVinduced apoptosis. J. Pineal Res. 40: 18-26 (2006)   DOI   ScienceOn
6 Afaq F, Mukhtar H. Botanical antioxidants in the prevention of photocarcinogenesis and photoaging. Exp. Dermatol. 15: 678-684 (2006)   DOI   ScienceOn
7 Lee YS, Jin DQ, Beak SM, Lee ES, Kim JA. Inhibition of ultraviolet-A-modulated signaling pathways by asiatic acid and ursolic acid in HaCaT human keratinocytes. Eur. J. Pharmacol. 476:173-178 (2003)   DOI   ScienceOn
8 Park CH, Lee MJ, Kim JP, Yoo ID, Chung JH. Prevention of UV radiation-induced premature skin aging in hairless mice by the novel compound melanocin A. Photochem. Photobiol. 82: 574-578 (2006)   DOI   ScienceOn
9 Moon HI, Lee J, Zee OP, Chung JH. Flavonoid compounds from Viola hondoensis and their effect on matrix metalloproteinase-1 in ultraviolet irradiation of cultured human skin fibroblasts. Food Sci. Biotechnol. 14: 143-146 (2005)
10 Nishi J, Ogura R, Sugiyama M, Hidaka T, Kohno M. Involvement of active oxygen in lipid peroxide radical reaction of epidermal homogenate following ultraviolet light exposure. J. Invest. Dermatol. 97: 115-119 (1991)   DOI   ScienceOn
11 Nakamura T, Ishikawa T, Nanashima N, Miura T, Nozaka H, Nakaoka R, Sato T. 4-Methylumbelliferone induces the expression of membrane type 1-matrix metalloproteinase in cultured human skin fibroblasts. Biochem. Bioph. Res. Co. 298: 646-650 (2002)   DOI   ScienceOn
12 Jagetia GC, Reddy TK, Venkatesha VA, Kedlaya R. Influence of naringin on ferric iron induced oxidative damage in vitro. Clin. Chim. Acta 347: 189-197 (2004)   DOI   ScienceOn
13 F'guyer S, Afaq F, Mukhtar H. Photochemoprevention of skin cancer by botanical agents. Photodermatol. Photo. 19: 56-72 (2003)   DOI   ScienceOn
14 Philips N, Smith J, Keller T, Gonzalez S. Predominant effects of Polypodium leucotomos on membrane integrity, lipid peroxidation, and expression of elastin and matrixmetalloproteinase-1 in ultraviolet radiation exposed fibroblasts, and keratinocytes. J. Dermatol. Sci. 32: 1-9 (2003)   DOI   ScienceOn
15 Ryoo YW, Suh SI, Mun KC, Kim BC, Lee KS. The effects of the melatonin on ultraviolet-B irradiated cultured dermal fibroblasts. J. Dermatol. Sci. 27: 162-169 (2001)   DOI   ScienceOn
16 Cimino F, Ambra R, Canali R, Saija A, Virgili F. Effect of cyaniding-3-O-glucoside on UVB-induced response in human keratinocytes. J. Agr. Food Chem. 54: 4041-4047 (2006)   DOI   ScienceOn
17 Catani MV, Rossi A, Costanzo A, Sabatini S, Levrero M, Melino G, Avigliano L. Induction of gene expression via activator protein-1 in the ascorbate protection against UV-induced damage. Bichem. J. 356: 77-85 (2001)   DOI   ScienceOn
18 Oh JH, Chung AS, Steinberenner H, Sies H, Brenneisen P. Thioredoxin secreted upon ultraviolet A irradiation modulates activities of matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in human dermal fibroblasts. Arch. Biochem. Biophys. 423: 218-226 (2004)   DOI   ScienceOn
19 Fisher GJ, Talwar HS, Lin J, Lin P, McPillips F, Wang Z, Li X, Wan Y, Kang S, Voorhees JJ. Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo. J. Clin. Invest. 101: 1432-1440 (1998)   DOI   ScienceOn
20 Aebi H. Catalase. Method Enzymol. 105: 125-126 (1985)
21 Chan WH, Wu CC, Yu JS. Curcumin inhibits UV irradiationinduced oxidative stress and apoptotic biochemical changes in human epidermoid carcinoma A431 cells. J. Cell Biochem. 90: 327-338 (2003)   DOI   ScienceOn
22 Jeong HJ, Koo HN, Na HJ, Kim MS, Hong SH, Eom JW, Kim KS, Shin TY, Kim HM. Inhibition of TNF-$\alpha$ and IL-6 production by aucubin through blockade of NF-$\kappa$B activation in RBL-2H3 mast cells. Cytokine 18: 252-259 (2002)   DOI   ScienceOn
23 Rodriguez J, Yanez J, Vicente V, Alcaraz M, Benavente-Garcia O, Castillo J, Lorente J, Lozano JA. Effects of several flavonoids on the growth of B16F10 and SK-MEL-1 melanoma cell lines:Relationship between structure and activity. Melanoma Res. 12: 99-107 (2002)   DOI   ScienceOn
24 Rabe JH, Mamelak AJ, McElgunn PJS, Morison WL, Sauder DN. Photoaging: Mechanisms and repair. J. Am. Acad. Dermatol. 55: 1-19 (2006)   DOI   ScienceOn
25 McCord J, Fridovich I. Superoxide dismutase. An enzymatic function for erythrocuprein (heterocuprein). J. Biol. Chem. 244: 6049-6055 (1969)   PUBMED
26 Fusening NE, Boukamp P. Multiple stages and genetic alterations in immortalization, malignant transformation, and tumor progression of human skin keratinocytes. Mol. Carcinogen. 23: 144-158 (1998)   DOI   ScienceOn
27 Afaq F, Adhami VM, Mukhtar H. Photochemoprevention of ultraviolet B signaling and photocarcinogenesis. Mutat. Res. 571:153-173 (2005)   DOI   PUBMED
28 Dissemond J, Schneider LA, Brenneisen P, Briviba K, Wenk J, Wlaschek M, Scharffetter-Kochanek K. Protective and determining factors for the overall lipid peroxidation in ultraviolet A1-irradiated fibroblasts: in vitro and in vivo investigations. Brit. J. Dermatol. 149:341-349 (2003)   DOI   ScienceOn
29 Sternlicht MD, Werb Z. How matrix metalloproteinases regulate cell behavior. Annu. Rev. Cell Dev. Bi. 17: 463-516 (2001)   DOI   ScienceOn
30 Yen GC, Hsieh CL. Antioxidant activity of extracts from Du-Zhong (Eucommia ulmoides Oliv.) toward various lipid peroxidation models in vitro. J. Agr. Food Chem. 46: 3952-3957 (1998)   DOI   ScienceOn
31 Katiyar SK, Ahmad N, Mukhtar H. Green tea and skin. Arch. Dermatol. 136: 989-994 (2000)   DOI   ScienceOn
32 Ho JN, Lee YH, Lee YD, Jun WJ, Kim HK, Hong BS, Shin DH, Cho HY. Inhibitory effects of aucubin isolated from Eucommia ulmoides against UVB-induced matrix metalloproteinase-1 production in human skin fibroblasts. Biosci. Biotech. Bioch. 69: 2227-2231 (2005)   DOI   ScienceOn
33 Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase, and glutathione S-transferase activities in rat lung and liver. Biochim. Biophys. Acta 582: 67-78 (1979)   DOI   PUBMED   ScienceOn
34 Wang H, Kochevar IE. Involvement of UVB-induced reactive oxygen species in TGF-$\beta$ biosynthesis and activation in keratinocytes. Free Radical Bio. Med. 38: 890-897 (2005)   DOI   ScienceOn
35 Na MK, Min BS, An RB, Song KS, Seong YH, Bae K. Effect of Astilbe koreana on ultraviolet B (UVB)-induced inflammatory response in human keratinocytes. Biol. Pharm. Bull. 27: 1301-1304 (2004)   DOI   ScienceOn
36 Weisiger RA, Fridovich I. Superoxide dismutase: Organelle specificity. J. Biol. Chem. 248: 3582-3592 (1973)   PUBMED
37 Jones SA, McArdle F, Jack CI, Jackson MJ. Effect of antioxidant supplementation on the adaptive response of human skin fibroblasts to UV-induced oxidative stress. Redox. Rep. 4: 291-299 (1999)   DOI   ScienceOn
38 Fisher GJ, Datta SC, Talwar HS, Wang ZQ, Varani J, Kang S, Voorhees JJ. Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature 379: 335-339 (1996)   DOI   ScienceOn
39 Sudel KM, Venzke K, Knussmann-Hartig E, Moll I, Stab F, Wenck H, Wittern K, Gercken G, Gallinat S. Tight control of matrix metalloproteinase-1 activity in human skin. Photochem. Photobiol. 78: 355-360 (2003)   DOI   ScienceOn
40 Tarozzi A, Marchesi A, Hrelia S, Angeloni C, Andrisano V, Fiori J, Cantelli-Forti G, Hrelia P. Protective effects of Cyanidin-3-O-$\beta$-glucopyranoside against UVA-induced oxidative stress in human keratinocytes. Photochem. Photobiol. 81: 623-629 (2005)   DOI   ScienceOn
41 Kim HH, Shin CM, Park CH, Kim KH, Cho KH, Eun HC, Chung JH. Eicosapentanoic acid inhibits UV-induced MMP-1 expression in human dermal fibroblasts. J. Lipid Res. 46: 1712-1720 (2005)   DOI   ScienceOn
42 Fischer TW, Scholz G, Knoll B, Hilper U-C, Elsner P. Melatonin reduces UV-induced reactive oxygen species in dose-dependent manner in IL-3-stimulated leukocytes. J. Pineal Res. 31: 39-45 (2001)   DOI   ScienceOn