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Aqueous Extracts of Walnut (Juglans regia L.) and Nelumbo nucifera Seeds Reduce Plasma Corticosterone Levels, Gastric Lesions, and c-fos Immunoreactivity in Chronic Restraint-stressed Mice  

Kim, Dae-Won (Central Research Center, Natural F&P Co., Ltd.)
Hwang, In-Koo (Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University)
Yoo, Ki-Yeon (Department of Anatomy and Neurobiology, College of Medicine, Hallym University)
Li, Hua (Department of Anatomy and Neurobiology, College of Medicine, Hallym University)
Kang, Il-Jun (Department of Food Science and Nutrition, Division of Life Science, Hallym University)
Moon, Won-Kuk (Central Research Center, Natural F&P Co., Ltd.)
Won, Moo-Ho (Department of Anatomy and Neurobiology, College of Medicine, Hallym University)
Kim, Seok-Joong (Department of Food and Nutrition, Dongduk Women's University)
Han, Dae-Seok (Food Processing and Post-harvest Technology Division, Korea Food Research Institute)
Kim, Dong-Woo (Central Research Center, Natural F&P Co., Ltd.)
Publication Information
Food Science and Biotechnology / v.17, no.4, 2008 , pp. 713-717 More about this Journal
Abstract
In the present study, chronic effects of the hot water extracts of walnut seed (Juglans regia L.) (WSE) and Nelumbo nucifera seed (NSE) were investigated in mice exposed to 2 hr of restraint stress each day for 4 weeks. Corticosterone levels in serum were significantly increased in the vehicle-treated stressed group ($25\;{\mu}g/dL$) compared to that in the control group ($13\;{\mu}g/dL$). This stress induced gastric redness and lesions. However, treatment with WSE and/or NSE significantly protected the stomach from this lesion by 50-60% compared to that in the vehicletreated group. In the amygdala, the administration of WSE and/or NSE also reduced the immediate early gene (c-fos) expression by 70-90% vs. the vehicle-treated group. These suggest that WSE and/or NSE may reduce the appearance of symptoms induced by stress and these materials are useful as anti-stress foods, as natural products tend to be relatively safe compared to chemical products.
Keywords
restraint stress; corticosterone; gastric lesion; c-fos; medial amygdale;
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