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Comparative Analysis of Tagatose Productivity of Immobilized L-Arabinose Isomerase Expressed in Escherichia coli and Bacillus subtilis  

Cheon, Ji-Na (Department of Biotechnology, Catholic University of Korea)
Kim, Seong-Bo (Food Ingredient Center, CJ Foods R&D, CJ Corp.)
Park, Seong-Won (Food Ingredient Center, CJ Foods R&D, CJ Corp.)
Han, Jong-Kwon (Food Ingredient Center, CJ Foods R&D, CJ Corp.)
Kim, Pil (Department of Biotechnology, Catholic University of Korea)
Publication Information
Food Science and Biotechnology / v.17, no.3, 2008 , pp. 655-658 More about this Journal
Abstract
Although arabinose isomerase (E.C. 5.3.1.4), a commercial enzyme for edible tagatose bioconversion, can be expressed in an Escherichia coli system, this expression system might leave noxious by-products in food. To develop an eligible tagatose bioconversion with food-safe system, we compared the tagatose production activity of immobilized arabinose isomerase expressed in Bacillus subtilis (a host generally recognized as safe) with that of the enzyme expressed in E. coli. A 48% increase in tagatose production (4.3 g tagatose/L at $69.4\;mg/L{\cdot}hr$) was found using the B. subtilis-expressed immobilized enzyme system, compared to the E. coli-expressed enzyme system (2.9 g tagatose/L). The increased productivity with safety of the B. subtilis-expressed arabinose isomerase suggests that it is a more eligible candidate for commercial tagatose production.
Keywords
tagatose bioconversion; food safety; expression host; Bacillus subtilis; Escerichia coli;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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