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Multiplex PCR Detection for 3 Events of Genetically Modified Maize, DAS-59122-7, TC6275, and MIR604  

Ahn, Ji-Hye (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University)
Kim, Jae-Hwan (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University)
Kim, Su-Youn (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University)
Lee, Woo-Young (BioFood Team, Korea Food & Drug Administration)
Park, Sun-Hee (BioFood Team, Korea Food & Drug Administration)
Kim, Hae-Yeong (Institute of Life Sciences and Resources and Graduate School of Biotechnology, Kyung Hee University)
Publication Information
Food Science and Biotechnology / v.17, no.3, 2008 , pp. 569-572 More about this Journal
Abstract
A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 3 events of genetically modified (GM) maize. The event-specific primers were used to discriminate the following 3 events of GM maize (DAS-59122-7, TC6275, and MIR604) using multiplex PCR method. The zein gene was used as an endogenous maize reference gene in the multiplex PCR detection. The primer pair Zein-FIR producing a 99 bp amplicon was used to amplify the zein gene. The primer JI-Das-F1/R1 for DAS-59122-7, JI-TC6275-F3/R3 for TC6275, and JI-MIR F1/R1 for MIR604 yielded an amplicon of 130, 162, and 197 bp, respectively. The detection limit of multiplex PCR was 1% for DAS-59122-7, TC6275, and MIR604 for one reaction.
Keywords
DAS-59122-7; TC6275; MIR604; maize; multiplex polymerase chain reaction (PCR);
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 4  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
연도 인용수 순위
1 Heo M-S, Kim J-H, Park S-H, Woo G-J, Kim H-Y. Detection of genetically modified maize by multiplex PCR method. J. Microbiol. Biotechn. 14: 1150-1156 (2004)
2 European Commission Regulation (EC) 1829/2003 and 1830/ 2003. Off. J. Eur. Commun. L 268: 1-28 (2003)
3 Notification 1775. Food and Marketing Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan, Tokyo, Japan (2000)
4 Hernandez M, Rodriguez-Lazaro D, Zhang D, Esteve T, Pla, M, Prat S. Interlaboratory transfer of a PCR multiplex method for simultaneous detection of four genetically modified maize events: Bt11, MON810, T25, and GA21. J. Agr. Food Chem. 53: 3333- 3337 (2005)   DOI   ScienceOn
5 Anklam E, Gadani F, Heinze P, Pijnenburg H, Van den Eede G. Analytical methods for detection and determination of genetically modified organism in agricultural crops and plant derived food products. Eur. Food Res. Technol. 214: 3-26 (2002)   DOI   ScienceOn
6 Kim J-H, Song H-S, Heo M-S, Lee W-Y, Lee S-H, Park S-H, Park H-K, Kim M-C, Kim H-Y. Detection of eight different events of genetically modified maize by multiplex PCR method. Food Sci. Biotechnol. 15: 148-151 (2006)   과학기술학회마을
7 Notification 2000-31. Ministry of Agriculture and Forestry of Korea, Seoul, Korea (2000)
8 Kim J-H, Jee S-M, Park C-S, Kim H-Y. Detection of transgenic rice containing cry1Ac gene derived from Bacillus thuringiensis by PCR. Food Sci. Biotechnol. 15: 625-630 (2006)   과학기술학회마을
9 James D, Schmidt AM, Wall E, Green M, Masri S. Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis. J. Agr. Food Chem. 51: 5829-5834 (2003)   DOI   ScienceOn
10 Bruderer S, Leitner KE. Genetically Modified (GM) Crops: Molecular and regulatory details. Version 2. BATS, 30/06/2003. Available from: http://www.bats.ch/gmo-watch/GVO-report140703. pdf. Accessed May 20, 2006
11 Holst-Jensen A, Ronning SB, Lovseth A. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem. 375: 985-993 (2003)   DOI
12 Agbios data base product description (DAS-59122-7, TC6275, and MIR604). Available from: http://www.Agbios.com. Accessed May 13, 2007
13 Ahmed FE. Detection of genetically modified organisms in foods. Trends Biotechonol. 20: 215-223 (2002)   DOI   ScienceOn
14 Yang L, Pan A, Zhang H, Guo J, Yin C, Zhang D. Event-specific qualitative and quantitative polymerase chain reaction analysis for genetically modified canola T45. J. Agr. Food Chem. 54: 9735-9740 (2006)   DOI   ScienceOn
15 James C. Global status of commercialized biotech/GM crops. ISAAA Briefs No. 37. International Service for the Acquisition of Agri-biotech Applications, Ithaca, NY, USA (2007)
16 Kim J-H, Song H-S, Kim D-H, Kim H-Y. Quantification of genetically modified canola GT73 using TaqMan real-time PCR. J. Microbiol. Biotechn. 16: 1778-1783 (2006)   과학기술학회마을
17 Kim J-H, Kim T-W, Lee W-Y, Park S-H, Kim H-Y. Multiplex PCR detection of the GT73, MS8RF3, and T45 varieties of GM canola. Food Sci. Biotechnol. 16: 104-109 (2007)   과학기술학회마을