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Production and Application of Galacto-oligosaccharides from Lactose by a Recombinant $\beta$-Galactosidase of Bifidobacterium infantis Overproduced by Pichia pastoris  

Jung, Sung-Je (Department of Food Science and Agricultural Chemistry, McGill University)
Lee, Byong-Hoon (Department of Food Science and Agricultural Chemistry, McGill University)
Publication Information
Food Science and Biotechnology / v.17, no.3, 2008 , pp. 514-518 More about this Journal
Abstract
After overproduction of a recombinant $\beta$-galactosidase of Bifidobacterium infantis in Pichia pastoris, a synthesis of galacto-oligosaccharides (GOS) from 36% lactose using the enzyme (170.74 U/mg) was investigated. The transgalactosylation ratio reached up to 25.2% with 83.1% conversion of initial lactose and the maximum yield of GOS was 40.6%. The GOS syrup was composed of a 13.43% galacto-oligosaccharides, 5.06% lactose, and 8.76% monosaccharides. The prebiotic effect of GOS on the growth of bifidobacteria and lactobacilli strains was investigated in vitro. The maximum growth rate of Bifidobacterium breve and Lactobacillus acidophillus in GOS syrup (5%, v/v) media were 0.49 and 0.96/hr that are higher than those in 1%(w/v) galactose and 1%(w/v) lactose containing media. However, there was no significant difference between the specific growth rates of L. acidophillus in 1%(w/v) glucose and 5%(v/v) GOS syrup. Our data showed that GOS definitely promoted the growth of B. breve ATCC $15700^T$ and L. acidophilus ATCC 33323.
Keywords
galacto-oligosaccharide; Pichia pastoris; recombinant $\beta$-galactosidase; non-digestible oligosaccharide; Bifidobacterium; lactobacillus;
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1 Cummings JH, Macfarlane GR, Englyst HN. Prebiotic digestion and fermentation. Am. J. Clin. Nutr. 73: 415-420 (2001)   DOI
2 Matteuzzi D, Swennen E, Rossi M, Hartman T, Lebet V. Prebiotic effects of a wheat germ preparation in human healthy subjects. Food Microbiol. 21: 119-124 (2004)   DOI   ScienceOn
3 Moro G, Minoli I, Mosca M, Fanaro S, Jelinek J, Stahl B. Dosage related bifidogenic effects of galacto- and fructo-oligosaccharides in formula-fed term infants. J. Pediatr. Gastro Nutr. 34: 291-295 (2002)   DOI   ScienceOn
4 Hung MN, Xia Z, Lee BH. Molecular and biochemcial analysis of two beta-galactosidases from Bifidobacterium infantis HL96. Appl. Environ. Microb. 67: 4256-4263 (2001)   DOI   ScienceOn
5 Oh NS, Lee BH. Phytase properties from Bifidobacterium animalis. Food Sci. Biotechnol. 16: 580-583 (2007)   과학기술학회마을
6 Hiriyama K, Rafter J. The role of probiotic bacteria in cancer prevention. Microbes Infect. 2: 681-686 (2000)   DOI   ScienceOn
7 Sakamoto I, Igarashi M, Kimura K, Takagi A, Miwa T, Koga Y. Suppressive effect of Lactobacillus gasseri OLL 2716 (LG 21) on Helicobacter pylori infection in humans. J. Antimicrob. Chemoth. 47: 709-710 (2001)   DOI   ScienceOn
8 Swennen K, Courtin CM, Delcour JA. Non-digestible oligosaccharides with prebiotic properties. Crit. Rev. Food. Sci. 46: 459-471 (2006)   DOI   ScienceOn
9 Mikkelsen LL, Jakobsen M, Jensen BB. Effects of dietary oligosaccharides on microbial diversity and fructo-oligosaccharide degrading bacteria in faeces of piglets post-weaning. Anim. Feed. Sci. Tech. 109: 133-150 (2003)   DOI   ScienceOn
10 Mussatto SI, Mancilha IM. Non-digestible oligosaccharides: A review. Carbohyd. Polym. 68: 587-597 (2007)   DOI   ScienceOn
11 Smart J. Pillidge CJ, Garman JH. Growth of lactic acid bacteria and bifidobacteria on lactose and lactose-related mono-, di-, and trisaccharide and correlation with distribution of $\beta$-galactosidase and phosphore-$\beta$-galactosidase. J. Dairy Res. 60: 557-568 (1993)   DOI   ScienceOn
12 In MJ, Kim MH, Jung J. Isolation of Bacillus sp. producing $\beta$- galactosidase with high transgalactosylation activity and its culture characteristics regarding enzyme production. J. Korean Soc. Agric. Chem. Biotechnol. 38: 502-506 (1995)
13 Xiao JZ, Kondo S, Takahashi N, Miyajik K, Oshida K, Hiramatsu A, Iwatsuki K, Kokubo S, Hosono A. Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. J. Dairy. Sci. 86: 2452-2461 (2003)   DOI   ScienceOn
14 Marcozet M, Simpson B, Lee BH. Expression of a cold-adapted fish trypsin in Pichia pastoris. FEMS Yeast Res. 5: 851-857 (2005)   DOI   ScienceOn
15 Sako T, Matsumoto K, Tanaka R. Recent progress on research and applications of non-digestible galacto-oligosaccharides. Int. Dairy. J. 9: 69-80 (1999)   DOI   ScienceOn
16 Shin HJ, Yang JW. Enzymatic production of galactooligosaccharide by Bullera singularis $\beta$-galactosidase. J. Microbiol. Biotechn. 8: 484-489 (1998)
17 Atwal A, Bisakowski B, Richard S, Robert N, Lee B. Cloning and expression of hydroperoxide lyase genes from tomato (Lycopersicon esculentum) and red bell pepper (Capsicum annum) in Pichia pastoris. Process Biochem. 40: 95-102 (2005)   DOI
18 Choi YJ, Miguez CB, Lee BH. Characterization and heterologous gene expression of a novel esterase from Lactobacillus casei CL96. Appl. Environ. Microb. 70: 3213-3221 (2004)   DOI   ScienceOn
19 Rinne MM, Gueimonde M, Kalliomaki M, Hoppu U, Salminen SJ, Isolauri E. Similar bifidogenic effects of prebiotic supplemented partially hydrolyzed infant formula and breastfeeding on infant gut microbiota. FEMS Immunol. Med. Mic. 43: 59-65 (2005)   DOI   ScienceOn
20 Choi NY, Shin HS. Effect of oligosaccharides and inulin on the growth and viability of bifidobacteria in skim milk. Food Sci. Biotechnol. 15: 543-548 (2006)   과학기술학회마을
21 Petzelbauer I, Zeleny R, Reiter A, Kulbe KD, Nidetzky B. Development of an ultra-high-temperature process for the enzymatic hydrolysis of lactose: II. Oligosaccharide formation by two thermostable $\beta$-glycosidases. Biotechnol. Bioeng. 69: 140-149 (2000)   DOI   ScienceOn
22 Playne MJ, Bennett LE, Smithers GW. Functional dairy foods and ingredients. Aust. J. Dairy Technol. 58: 242-264 (2003)
23 Boehm G, Jelinek J, Stahl B, Van Laere K, Knol J. Prebiotics in infant formulas. J. Clin. Gastroenterol. 38: S76-S79 (2004)   DOI   ScienceOn
24 Huebner J, Wehling RL, Hutkins RW. Functional activity of commercial prebiotics. Int. Dairy. J. 17: 770-775 (2007)   DOI   ScienceOn
25 Gopal PK, Sullivan PA, Smart JB. Utilization of galactooligosaccharides as selective substrates for growth by lactic acid bacteria including Bifidobacterium lactis DR10 and Lactobacillus rhamnosus DR20. Int. Dairy J. 11: 19-25 (2001)   DOI   ScienceOn
26 Lim SI, Kim GB, YI SH, Lee BH. Characteristics of a Bifidobacterium longum LL04 $\beta$-galactosidase (recombinant) produced in Escherichia coli. Food Sci. Biotechnol. 6: 908-913 (2006)   과학기술학회마을
27 Steer T, Carpenter H, Tuohy K. Gibson GR. Perspectives on the role of the human gut microbiota in health and methods of study. Nutr. Res. Rev. 13: 229-254 (2000)   DOI   ScienceOn
28 Chockchaisawasdee S, Athanasopoulos VI, Niranjan K, Rastall RA. Synthesis of galactooligosaccharide from lactose using $\beta$- galactosidase from Kluyveromyces lactis: Studies on batch and continuous UF membrane-fitted bioreactors. Biotechnol. Bioeng. 89: 434-443 (2004)   DOI   ScienceOn
29 Ishikawa F, Takayama H, Suguri T, Matsumoto K, Ito M, Chonana O, Deguchi Y, Kikuci H, Watanuki M. Effects of $\beta$-1-4 linked galacto-oligosaccharides on human fecal microflora. Bifidus 9: 5-18 (1995)
30 Marteau P, Flourié B. Tolerance to low-digestible carbohydrates: Symtomatology and methods. Brit. J. Nutr. 85: 817-821 (2001)
31 Tanaka R. Matsumoto K. Recent progress on probiotics in Japan, including galacto-oligosaccharides. Bull. Int. Dairy Fed. 336: 21-27 (1998)
32 Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: Introducing the concept of prebiotics. J. Nutr. 125: 1401-1412 (1995)
33 Orrhage KM, Annas A, Nord CE, Brittebo EB, Rafter JJ. Effects of lactic acid bacteria on the food mutagen Trp-P-2 in mice. Scand. J. Gastroentero. 37: 215-221 (2002)   DOI   ScienceOn
34 Yanahira S, Kobayashi T, Suguri T, Nakakoshi M, Miura S, Ishikawa S, Nakajima L. Formation of oligosaccharides from lactose by Bacillus circulans beta-galactosidase. Biosci. Biotech. Bioch. 59: 1021-1026 (1995)   DOI   ScienceOn
35 de Roos NM, Katan MB. Effects of probiotic bacteria on diarrhea, lipid metabolism, and carcinogenesis: A review of papers published between 1988 and 1998. Am. J. Clin. Nutr. 71: 405-411 (2000)   DOI
36 Morishita Y, Oowada T, Ozaki A, Mizutani T. Galactooligosaccharide in combination with Bifidobacterium and Bacteroides affects the population of Clostridium perfringens in the intestine of gnotobiotic mice. Nutr. Res. 22: 1333-1341 (2002)   DOI   ScienceOn