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Characterization of Polysaccharides Obtained from Purslane (Portulaca olerace L.) Using Different Solvents and Enzymes  

Choi, Ae-Jin (Food Nano-Bio Research Group, Korea Food Research Institute)
Kim, Chul-Jin (Food Nano-Bio Research Group, Korea Food Research Institute)
Cho, Yong-Jin (Food Nano-Bio Research Group, Korea Food Research Institute)
Kim, Yang-Ha (Department of Food and Nutritional Science, Ewha Womens University)
Cha, Jae-Yoon (CJ Corporation)
Hwang, Jae-Kwan (Department of Biotechnology, Yonsei University)
Kim, In-Hwan (Department of Food and Nutrition, Korea University)
Kim, Chong-Tai (Food Nano-Bio Research Group, Korea Food Research Institute)
Publication Information
Food Science and Biotechnology / v.16, no.6, 2007 , pp. 928-934 More about this Journal
Abstract
Physiochemical properties, such as yield and molecular weight distribution of polysaccharide fractions, of polysaccharides in the enzymatic hydrolysates of purslane were investigated and characterized. A higher amount of micro nutrients, such as potassium (9,413 mg/100 g), phosphorus acid (539 mg/100 g), leucine, alanine, lysine, valine, glycine, and isoleucine, was present in whole purslane. The yield of water soluble polysaccharides (WSP) was 0.29, 7.01, and 7.94% when extracted using room temperature water (RTW), hot-water (HW), and hot temperature/high pressure-water (HTPW), respectively, indicating that HW or HTPW extraction may be effective to obtain WSP from purslane. The average ratio of L-arabinose:D-galactose in the WSP was 37:49, 34:37, and 27:29, when extracted using RTW, HW, and HTPW, respectively. These results indicate that water was a suitable extraction solvent for preparation of the arabinogalactan component of whole purslane. A higher yield and total carbohydrate content was obtained by using Viscozyme L instead of Pectinex 5XL during extraction of the WSP, which indicates that enzymatic treatment of purslane may be an effective method to control the Mw of polysaccharides. Finally, it was confirmed that Viscozyme L is a suitable enzyme for the hydrolysis and separation of polysaccharides obtained from purslane.
Keywords
purslane (portulaca oleracea L.); physicochemical property; polysaccharide; isolation; arabinogalactan;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
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