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Microbiological and Chemical Detection of Antibiotic Residues in Livestock and Seafood Products in the Korean Market  

Park, Sung-Kug (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Kim, Mee-Hye (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Sho, You-Sub (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Chung, So-Young (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Hu, Soo-Jung (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Lee, Jong-Ok (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Hong, Moo-Ki (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Kim, Myung-Chul (Department of Food Safety Evaluation, Korea Food and Drug Administration)
Kang, Ju-Seop (Department of Pharmacology and Clinical Pharmacology Lab, Institute of Biomedical Sciences, College of Medicine, Hanyang University)
Jhee, Ok-Hwa (Department of Practical Arts Education, Gongju National University of Education)
Publication Information
Food Science and Biotechnology / v.16, no.6, 2007 , pp. 868-872 More about this Journal
Abstract
The microbiological and chemical identification of antibiotic residues was attempted for livestock and seafood products including pork (n=34), beef (n=34), chicken (n=32), flatfish (n=37), armorclad rockfish (n=36), and sea bream (n=27). The meat (n=100) and seafood (n=100) samples were collected from 9 markets in 5 major Korean cities. Antibiotic substances were identified from the classes of tetracyclines, macrolides, penicillins, aminoglycosides, polyethers, peptides, sulfonamides, quinolones, chlorampenicols, and novobiocins using a microbiological assay, the Charm II test and high performance liquid chromatography (HPLC) with ultra violet (UV) and fluorescence detectors. The results showed that 2 tetracyclines (oxytetracycline and tetracycline) and 3 quinolones (ciprofloxacin, norfloxacin, and enrofloxacin) were detected in 4 samples of flatfish among all 100 seafood samples tested. No antibiotic residues were detected in the 100 livestock product samples tested. The amounts (min-max, mg/kg) of the residual antibiotics were as follows; tetracycline 0.78-0.85, oxytetracycline 0.49-0.74, ciprofloxacin 0.09-0.83, norfloxacin 0.01-0.21, enrofloxacin 0.12-2.98. These data indicate that the total detection rate of antibiotics in livestock and seafood products was approximately 2%.
Keywords
microbiological identification; chemical identification; residual antibiotics;
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1 Higgins HC, McEvoy JDG, Lynas L, Fagan NP. Evaluation of a single plate microbiological growth inhibition assay as a screening test for the presence of antimicrobial agents in compound animal feedingstuffs at therapeutic and contaminating concentrations. Food Addit. Contam. 16: 543-554 (1999)   DOI
2 Yang S, Cha J, Carlson K. Simultaneous extraction and analysis of 11 tetracycline and sulfonamide antibiotics in influent and effluent domestic wastewater by solid-phase extraction and liquid chromatography- electrospray ionization tandem mass spectrometry. J. Chromatogr. A 1097: 40-53 (2005)   DOI   ScienceOn
3 Oda H, Fushimi F, Kato M, Kitagawa M, Araki K, Seki N. Ohkubo H. Microarray analysis of the genes induced by tetracycline-regulated expression of NDRF/NeuroD2 in P19 cells. Biochem. Biophys. Res. Co. 335: 458-468 (2005)   DOI   ScienceOn
4 Myllyniemi AL, Rannikko R, Lindfors E, Niemi A, Backman C. Microbiological and chemical detection of incurred penicillin G, oxytetracycline, enrofloxacin, and ciprofloxacin residues in ovine and porcine tissues. Food Addit. Contam. 17: 991-1000 (2000)   DOI
5 Quon DJ. Monitoring of domestic and imported eggs for veterinary drug residues by the Canadian Food Inspection Agency. J. Agr. Food Chem. 48: 6421-6427 (2000)   DOI   ScienceOn
6 CODEX Alimentarius Committee Report Series, No. ALINORM 05/28/31. pp.11-13. In: The 15th session of the CODEX Committee on Residues of Veterinary Drugs in Foods. October 26-29, Washington DC, USA (2004)
7 Goto T, Ito Y, Yamada S, Matsumoto H, Oka H. High-throughput analysis of tetracycline and penicillin antibiotics in animal tissues using electrospray tandem mass spectrometry with selected reaction monitoring transition. J. Chromatogr. A 1100: 193-199 (2005)   DOI   ScienceOn
8 CODEX Alimentarius Committee Report Series, No. ALINORM 99/31. pp. 3-9. In: The 11th Session of the CODEX Committee on Residues of Veterinary Drugs in Foods. September 15-18, Washington DC, USA (1998)
9 Food Code 2005. Korea Food and Drug Administration. Seoul, Korea. pp. 424-423 (2005)
10 Jung SC. Establishment of control system of antibiotics for livestocks. Ann. Rep. Korea Food Drug Admin. 7: 1113-1114 (2003)
11 De Ruyck H, De Ridder H, Van Renterghen R, Van Wambeke F. Validation of HPLC method of analysis of tetracycline residues in eggs and broiler meat and its application to a feeding trial. Food Addit. Contam. 16: 47-56 (1999)   DOI   ScienceOn
12 WHO Technical Report Series, No. 939. Evaluation of certain veterinary drug residues in food. pp. 2-4. In: The 66th Report of the Joint FAO/WHO Expert Committee on Food Additives. February 22-28, Rome, Italy (2006)
13 WHO Report, No. WHO/EMC/ZOO/97.4. The medical impact of the use of antimicrobials in food animals. In: Conclusion of WHO Meeting. October 13-17, Berlin, Germany (1997)
14 Heo GJ, Shin KS, Lee MH. Diseases of aquaculture animals and prevention of drug residues. Korean J. Food Hyg. 7: 7-19 (1992)
15 Sczesny S, Nau H, Hamscher G. Residue analysis of tetracyclines and their metabolites in eggs and in the environment by HPLC coupled with a microbiological assay and tandem mass spectrometry. J. Agr. Food Chem. 51: 697-703 (2003)   DOI   ScienceOn
16 Linton R, Lange M, Kennedy. Occurrence of antibiotics and other inhibitory substance in heat and eggs. J. Antibiot. 30: 73-77 (1978)
17 Myllyniemi AL, Rintala R, Backman C, Niemi A. Microbiological and chemical identification of antimicrobial drugs in kidney and muscle samples of bovine cattle and pigs. Food Addit. Contam. 16: 339-351 (1999)   DOI
18 Pellinen T, Bylund G, Virta M, Niemi A, Karp M. Detection of traces of tetracyclines from fish with a bioluminescent sensor strain incorating bacterial luciferase reporter gene. J. Agr. Food Chem. 50: 4812-4815 (2002)   DOI   ScienceOn