Browse > Article

Phenolic Antioxidants Isolated from Mulberry Leaves  

Kim, Young-Chan (Korea Food Research Institute)
Kim, Mi-Yeon (Korea Institute of Science and Technology for Eastern Medicine (KISTEM), Neumed Co., Ltd.)
Takaya, Yoshiaki (Faculty of Pharmacy, Meijo University)
Niwa, Masatake (Faculty of Pharmacy, Meijo University)
Chung, Shin-Kyo (Department of Food Science and Technology, Kyungpook National University)
Publication Information
Food Science and Biotechnology / v.16, no.5, 2007 , pp. 854-857 More about this Journal
Abstract
In this study, the crude methanol extract of mulberry leaves was fractionated with chloroform, ethyl acetate, n-butanol, and water, successively. The antioxidant activities of the fractions were examined with the 2-deoxyribose oxidation and linoleic acid peroxidation methods. The ethyl acetate fraction showed the strongest antioxidant activity. From it we isolated chlorogenic acid, caffeic acid, quercetin $3-O-{\beta}-D-glucopyranoside$, and kaempferol $3-O-{\beta}-D-glucopyranoside$ with preparatory octadecyl silane-high performance liquid chromatography (ODS-HPLC), and identified the compounds by nuclear magnetic resonance (NMR) and fast atom bombardment mass (FAB-MS) analyses. Overall, quercetin $3-O-{\beta}-D-glucopyranoside$ showed the strongest antioxidant activity by both the 2-deoxyribose oxidation and rat liver microsome peroxidation methods.
Keywords
quercetin $3-O-{\beta}-D-glucopyranoside$; chlorogenic acid; mulberry leave; antioxidant activity;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
연도 인용수 순위
1 Naitoh K. Studies on the micro constituent in mulberry leaves; Part 2. Isolation of rutin and quercetin from mulberry leaves. Nippon Nog. Kag. Kaishi 42: 450-453 (1969)
2 Kim SY, Gao JJ, Lee WC, Ryu KS, Lee KR, Kim YC. Antioxidative flavonoids from the leaves of Morus alba. Arch. Pharm. Res. 22: 81-85 (1999)   DOI   ScienceOn
3 Yagi M, Kouno T, Aoyagi Y, Murai H. The structure of Moraoline, a piperidine alkaloid from Morus species. Nippon Noug. Kaga. Kaishi 50: 571-572 (1976)   DOI
4 Markham KR, Terani B, Stanley R, Geiger H, Marby TJ. Carbon-13 NMR studies of flavonolds-III. Tetrahedron 34: 1389-1397 (1978)   DOI   ScienceOn
5 Chung SK, Osawa T, Kawakishi S. Hydroxyl radical scavenging effect of spices and scavengers from brown mustard (Brassica nigra). Biosci. Biotech. Bioch. 61: 118-123 (1997)   DOI   ScienceOn
6 Choi EJ, Lee YS, Kim GH. Antioxidative characteristics of extracts from aromatic herb Elsholtzia splendens. Food Sci. Biotechnol. 16: 489-492 (2007)   과학기술학회마을
7 Enkhmaa B, Shiwaku K, Katsube T, Kitajima K, Anuurad E, Yamasaki M, Yamane Y. Mulberry (Morus alba L.) leaves and their major flavonol quercetin 3-(6-malonylglucoside) attenuate atherosclerotic lesion development in LDL receptor-deficient mice. J. Nutr. 134: 729-734 (2004)   DOI
8 Choi JM, Han J, Yoon BS, Chung JH, Shin DB, Lee SK, Hwang JK, Ryang R. Antioxidant properties of tannic acid and its inhibitory effects on paraquat-induced oxidative stress in mice. Food Sci. Biotechnol. 15: 728-734 (2006)   과학기술학회마을
9 Kim HJ, Chang EJ, Cho SH, Chung SK, Park HD, Choi SW. Antioxidative activity of resveratrol and its derivatives isolated from seeds of Paeonia lactrflora. Biosci. Biotech. Bioch. 66: 1990-1993 (2002)   DOI   ScienceOn
10 Doi K, Kojima T, Makino M, Horiguchi Y, Kimura Y, Fujimoto Y. Studies on the constituents of the leaves of Morus alba L. Chem. Pharm. Bull. 49: 151-153 (2001)   DOI   ScienceOn
11 Nakatani N, Kikuzaki H. A new antioxidative glucoside isolated oregano (Origanum vulgare L.). Agr. Biol. Chem. Tokyo 51: 2727-2732 (1987)   DOI
12 Chen FJ, Nakashima N, Kimura I, Kimura M. Hypoglycemic activity and mechanism of extracts from mulberry leaves and cortex mori radices in streptozotocin induced diabetic mice. Yakuga. Zasshi 115: 476-482 (1955)
13 Hanner AN, Sharma HM, Kauffman EM, Newman HA. In vitro and in vivo inhibition of microsomal lipid peroxidation by MA-631. Pharmacol. Biochem. Be. 39: 505-510 (1994)
14 Onogi A, Osawa K, Yasuda H, Sakai A, Morita H. Tokawa H. Flavonol glycosides from the leaves of Morus alba. Shoyakuga. Zasshi 47: 423-425 (1993)
15 Kuiroka A, Yamazaki M. Purification and identification of flavonoids from the yellow green cocoon shell (sasamayu) of the silkworm (Bombyx mori). Biosci. Biotech. Bioch. 66: 1396-1399 (2002)   DOI   ScienceOn
16 Lee JY, Moon SO, Kwon YJ, Rhee SJ, Park HR, Choi SW. Identification and quantification of anthocyanins and flavonoids in mulberry (Morus sp.) cultivars. Food Sci. Biotechnol. 13: 176-184 (2004)
17 Nakatani N, Kayano S, Kikuzzaki H, Sumino K, Katagiri K, Mitani T. Identification, quantative determination, and antioxidative activities of chlorogenic acid isomers in prune (Prunus domestica L.). J. Agr. Food Chem. 48: 5512-5516 (2000)   DOI   ScienceOn
18 Guo J, Wang MH. Antioxidant and antidiabetic activities of Ulmus davidiana extracts. Food Sci. Biotechnol. 16: 55-61 (2007)   과학기술학회마을
19 Katsube T, Imawaka N, Kawano, Y, Yamazaki Y, Shiwaku K, Yamane Y. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem. 97: 25-31 (2006)   DOI   ScienceOn
20 Doi K, Kojima T, Fujimoto Y. Mulberry leaf extract inhibits the oxidative modification of rabbit and human low density lipoprotein. Biol. Pharm. Bull. 23: 1066-1071 (2000)   DOI   ScienceOn