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Antioxidant Activity of Partially Purified Extracts Isolated from Bacillus polyfermenticus SCD Culture  

Kim, Tae-Hoon (Division of Animal Life Science, Konkuk University)
Lee, Na-Kyoung (Division of Animal Life Science, Konkuk University)
Chang, Kyung-Hoon (Division of Animal Life Science, Konkuk University)
Park, Eun-Ju (Departrnent of Food Nutrition, Kyungnam University)
Choi, Shin-Yang (Korea Food Research Institute)
Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University)
Publication Information
Food Science and Biotechnology / v.15, no.3, 2006 , pp. 482-484 More about this Journal
Abstract
The antioxidant activity of Bacillus polyfermenticus SCD was studied by partially purified culture extracts using various methods: ammonium sulfate precipitation, adsorption to Diaion HP-20 columns using polar solvents, and extraction using non-polar solvents. The 1,1-diphenyl-2-picyrylhydrazyl (DPPH) radical scavenging activity of these partially purified fractions was then investigated. The precipitate isolated using 75%-saturated ammonium sulfate was shown to contain about 77.2% DPPH radical scavenging activity. Using the Diaion HP-20 resin adsorption method, the fraction obtained using 60% ethanol and 60% methanol possessed 76.7 and 89.5% DPPH radical scavenging activity, respectively. Fractions obtained by extracting with the non-polar solvents 80 mg/mL chloroform, 80 mg/mL n-hexane, 80 mg/mL ethyl acetate, and 80 mg/mL butanol contained 68.4, 75.0, 70.7, and 87.5% DPPH radical scavenging activity, respectively. Further study is needed to characterize the antioxidant substance(s) released by B. polyfermenticus SCD cultures.
Keywords
Bacillus polyfermenticus SCD; antioxidant activity; DPPH; Diaion HP-20;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
Times Cited By Web Of Science : 6  (Related Records In Web of Science)
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