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Antioxidant Activity of Extracts from Dangyuja (Citrus grandis Osbeck) Fruits Produced in Jeju Island  

Lim, Hee-Kyoung (Department of Medicine, Cheju National University)
Yoo, Eun-Sook (Department of Medicine, Cheju National University)
Moon, Jeong-Yong (Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University)
Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University)
Cho, So-Mi K. (Facuity of Biotechnology, College of Applied Life Sciences, Cheju National University)
Publication Information
Food Science and Biotechnology / v.15, no.2, 2006 , pp. 312-316 More about this Journal
Abstract
Crude extract of young dangyuja (Citrus grandis Osbeck) fruit was investigated for its antioxidant activity as measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. Among the samples, including 4 Citrus species and various solvent-extracted-fractions of young dangyuja fruit, the water-extracted fraction (WF) and butanol-extracted fraction (BF) showed the greatest DPPH free radical scavenging activity. WF and BF were further examined for their antioxidant activities by three different in vitro assays. The cell viability tests using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay showed that both fractions significantly reduced $H_2O_2$-induced cytotoxicity in HepG2 cells dose-dependently. Generation of the reactive oxygen species (ROS) was also reduced in cells pretreated with both fractions. In addition, BF showed a higher level of lipid peroxidation inhibitory capacity than WF in $H_2O_2$-treated HepG2 cells. Taken together, these results indicate that young dangyuja fruit can be used as an easily accessible source of natural antioxidants.
Keywords
Citrus grandis Osbeck; antioxidant activity; radical scavenging; reactive oxygen species; HepG2 cells;
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Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 23  (Related Records In Web of Science)
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