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Antioxidant Property of Genistein: Inhibitory Effect on HOCI Induced Protein Degradation, DNA Cleavage, and Cell Death  

Choi, Je-Min (Department of Biotechnology, College of Engineering, Yonsei University)
Ryu, Hyun-Jin (Department of Radiation Oncology, Asan Medical Center)
Chung, Jae-Hwan (Social Research and Data Analysis, World Research Corporation)
Park, Jae-Chul (Department of Biotechnology, College of Engineering, Yonsei University)
Hwang, Jae-Kwan (Department of Biotechnology, College of Engineering, Yonsei University)
Shin, Dong-Bum (Department of Food science and Nutrition, Cheju National University)
Lee, Sang-Kyou (Department of Biotechnology, College of Engineering, Yonsei University)
Ryang, Ryung (Department of Biotechnology, College of Engineering, Yonsei University)
Publication Information
Food Science and Biotechnology / v.14, no.3, 2005 , pp. 399-404 More about this Journal
Abstract
The aim of this study was to investigate the in vitro antioxidant profiles of genistein and other isoflavonoids. The reactivity of genistein towards stable radical and reactive oxygen species including ${\bullet}\;ABTS^+$, ${\bullet}{O_2}^-$, $H_2O_2$ and HOCl has been investigated, and the effects were compared with other isoflavonoids and antioxidants. All the tested isoflavonoids showed remarkable ${\bullet}\;ABTS^+$ scavenging activity and genistein was more potent than BHT and ascorbic acid. Genistein was more effective in scavenging hypochlorous acid than superoxide and hydrogen peroxide. At $10\;{\mu}M$ concentrations of genistein and genistin showed about 90% inhibitory effect on HOCl, while BHT and ascorbic acid showed lower than 50% inhibitory effect. Moreover, genistein could inhibit plasmid DNA cleavage, protein degradation and cell death from HOCl attack, while daidzein, BHT and ascorbic acid could not protect them effectively. These results suggest that genistein is a more potent radical scavenger than other isoflavonoids, and it can remarkably reduce cellular damage induced by HOCl.
Keywords
genistein; isoflavonoids; antioxidant; ROS; HOCl;
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Times Cited By Web Of Science : 7  (Related Records In Web of Science)
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  • Reference
1 Scavenging of reactive oxygen species bylectosteine, a molecule with two blocked-SH groups /
[ Gressier, B.;Lebegue, N. ] / Pharm. World Sci.   DOI
2 Genotoxicity of the isoflavones genistein, daidzein and equol in V79 cells /
[ Virgilio, A.L.;Iwani, K.;Watjen, W.;Kahl, R.;Degen, G.H. ] / Toxicol. Lett.
3 Evidence for genistein mediated cytotoxicity and apoptosis in rat brain /
[ Choi, E.J.;Lee, B.H. ] / Life Sci.   DOI
4 The selective effect of genistein on the toxicity of bleomycin in normal lymphocytes and HL-60 cells /
[ Lee, R.;Kim, Y.J.;Lee, Y.J.;Chung, H.W. ] / Toxicology
5 Neutrophil myeloperoxidase chlorinates and nitrates soy isoflavones and enhances their antioxidant properties /
[ Brenda, J.B.;Tracy, D.A.;Matthew, R.B.;Marion, K.;Landon, S.W.;Jeevan, P.;Nigel, P.B.;Stephen, B.;Victor, M.D.;Rakesh, P.P. ] / Free Radi. Biol. Med.   DOI
6 Chlorination and nitration of soy isoflavones /
[ Boersma, B.J.;Patel, R.P.;Kirk, M.;Jackson, P.L. ] / Arch. Biochem. Biophys.   DOI
7 Hypochlorous acid-induced lysis of human erythrocytes. Inhibition of cellular damage by the isoflavonoid genistein-8-C-glucoside /
[ Zavodnik, L.B.;Zavodnik, I.B.;Lapshina, E.A.;Shkodich, A.P.;Bryszewska, M.;Buko, V.U. ] / Biochemistry (Mosc)
8 Antioxidant activity applying an improved ABTS radical cation declorization assay /
[ Roberta, R.;Nicoletta, P.;Anna, P.;Min, Y.;Catherine, R.E. ] / Free Radi. Biol. Med.   DOI
9 The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen /
[ Nishikimi, M. ] / Biochem. Biophysic. Res. Communi.   DOI
10 The antioxidant action of taurine, hypotaurine, and therimetabolic precursors /
[ Aruoma, O.I. ] / Biochem. J.
11 The inhibitory effect of ambroxol on hypochlorous acid-induced tissue damage and respiratory burst of phagocytic cells /
[ Yong, C.;Yoon, Y.J. ] / Euro. J. Pharmacol.   DOI   ScienceOn
12 Different effects of genistein and resveratrol on oxidative DNA damage in vitro /
[ William, W.;Zhouxiao, C.;Xingxiang, P.;Michael, A.T.;Yunbo, L. ] / Mut. Res.   DOI
13 Soy isoflavones and cancer prevention /
[ Sarkar, F.H.;Li, Y. ] / Cancer Invest   DOI
14 Daidzein and genistein content of fruits and nuts /
[ Liggins, J.;Bluck, L.J.;Runswick, S.;Atkinson, C.;Coward, W.A.;Bingham, S.A. ] / J. Nutr. Biochem.   DOI
15 Isoflavone contents in some varieties of soybean /
[ Choi, J.S.;Kwon, T.W.;Kim, J.S. ] / Food Sci. Biotechnol.
16 Inhibitory effect of isoflavones on peroxynitrite-mediated low-density lipoprotein oxidation /
[ Lai, H.H.;Yen, G.C. ] / Biosci. Biotechnol. Biochem.   DOI
17 Different effects of genistein on molecular markers related to apoptosis in two phenotypically dissimilar breast cancer cell lines /
[ Xu, J.;Loo, G. ] / J. Cell Biochem.   DOI
18 Effect of genistein on cardiovascular responses to angiotensin n in conscious unrestrained rats /
[ Pesce, N.;Eyster, K.M.;Williams, J.L.;Wixon, R.;Wang, C.;Martin, D.S. ] / J. Cardiovasc. Pharmacol.   DOI
19 Isoflavones and skeletal health: are these molecules ready for clinical application? /
[ Migliaccio, S.;Anderson, J.J. ] / Osteoporos. Int.   DOI
20 The antioxidant activity of genistein in vitro /
[ Record, I.R.;Dreosti, I.E.;McInerney, J.K. ] / J. Nutri. Biochem.   DOI
21 Radical scavenging properties of genistein /
[ Zielonka, J.;Gbicki, J.;Grynkiewicz, G. ] / Free Radi. Biol. Med.   DOI
22 ESR and cell culture studies on free radical-scavenging and antioxidant activities of isoflavonoids /
[ Qiong, G.;Gerald, R.;Hadi, M.;Stefan, W.;Lester, P. ] / Toxicology   DOI   ScienceOn
23 Free Radicals in Biology and Medicine /
[ Halliwel, B.;Gutteridge, J.M.C. ] /
24 Mitochondrial free radical generation, oxidative stress and aging /
[ Enrique, C.;Kelvin, J.A.D. ] / Free Radi. Biol. Med.   DOI
25 Cellular resistance to vincristine suppresses NF-kappa B activation and apoptosis but enhances c-Jun-NH2-terminal protein kinase activation by tumor necrosis /
[ Giri, D.K.;Pantazis, P.;Aggarwal, B.B. ] / Apotosis   DOI   ScienceOn
26 Dietary intake of soy genistein is associated with lung function in patients with asthma /
[ Smith, L.J.;Holbrook, J.T.;Wise, R.;Blumenthal, M.;Dozor, A.J.;Mastronarde, J.;Williams, L. ] / J. Asthm.   DOI
27 Structure-antioxidant activity relationships of flavonoids and phenolic acids /
[ Catherine, A.R.;Nicholas, J.M.;George, P. ] / Free Radi. Biol. Med.   DOI
28 Oxidative stress-induced apoptosis is mediated by ERK1/2 phosphorylation /
[ Lee, Y.J.;Cho, H.N.;Soh, J.W.;Jhon, G.J.;Cho, C.K.;Chung, H.Y.;Bae, S.;Lee, S.J.;Lee, Y.S. ] / Exp. Cell Res.   DOI
29 Hydrogen peroxide signaling through tumor necrosis factor receptor I leads to selective activation of c-Jun N-terminal kinase /
[ Pantano, C.;Shrivastava, P.;McElhinney, B.;Janssen, H.Y. ] / J. Biol. Chem.   DOI
30 Activation of apurinic/apyrimidinic endonuclease in human cells by reactive oxygen species and its correlation with their adaptive response to genotoxicity of free radicals /
[ Chilakamarti, V.R.;Istvan, B.;Tadahide, I.;Sankar, M. ] / Proc. Nat. Acad. Sci. USA.
31 Antioxidant activity applying an improved ABTS radical cation decolorization assay /
[ Roberta, R.;Nicoletta, P.;Anna, P.;Ananth, P.;Min, Y.;Catherine, R.E. ] / Free Radi. Biol. Med.   DOI
32 Kinetics and mechanisms of HOCl reactions /
[ Folkes, L.K. ] / Arch. Biochem. Biophys.   DOI