[KSCI] Korea Science Citation Index Service

Mixed-type Inhibition of Human Hepatic Cytochrome P450 1-Catalyzed Ethoxyresorufin O-deethylation by Volatile Allyl Sulfides

Kim, Hyun-Jung (Korea Food Research Institute)
Chun, Hyang-Sook (Korea Food Research Institute)

Publication Information

Food Science and Biotechnology / v.14, no.2, 2005 , pp. 297-300 More about this Journal

Abstract

Effects of allyl sulfides on kinetic behavior of cytochrome P450 1 (CYP1)-catalyzed ethoxyresorufin O-deethylase (EROD) activity were studied using microsomes from benzo[a]pyrene-treated human hepatoma cells. Apparent $K_m$ and $V_{max}$ values were calculated as $2.8\;{\mu}M$ and $3.0\;{\mu}mol$ resorufin/min/mg protein based on Lineweaver-Burk plot of microsomal EROD activity, respectively. Diallyl disulfide (DADS) and diallyl trisulfide (DATS) affected $K_m$ and $V_{max}$ values of EROD activity and acted as mixed-type inhibitors for CYP1 isozymes. Apparent Ki values of DADS and DATS were calculated as 1.07 and 0.88 mM, respectively, by re-plotting slopes of Lineweaver-Burk plot and inhibitor concentrations.

Keywords

allyl sulfides; benzo[a]pyrene; cytochrome P450 1; human hepatoma cell; mixed-type inhibition;

Citations & Related Records

Times Cited By Web Of Science : 1 (Related Records In Web of Science)

Reference

1	Cytochrome P450 mediated reactions studied in genetically engineered V79 Chinese hamster cells / [ Doehmer, J.;Holtkamp, D.;Soballa, V.;Raab, G.;Schmalix, W.;Seidel, A.;Greim, H.;Jacob, J. ] / Pharmacogenetics
2	Cytochrome P4S0 isoform inhibitors as a tool for the investigation of metabolic reactions catalyzed by human liver microsomes / [ Bourrie, M.;Meunier, V.;Berger, Y.;Fabre, G. ] / J. Pharmacol. Exp. Ther.
3	Structure-activity relationships of organosulfur compounds as inhibitors of cytochrome P450 I-mediated activation of benzo[a]pyrene in a human cell model / [ Kim, H.J.;Chun, H.S. ] / Biotechnol. Lett.
4	The role of 12 eDNA-expressed human, rodent, and rabbit cytochrome P450 in the metabolism of benzo[a]pyrene and benzo(a]pyrene trans-7,8-dihydrodiol / [ Shou, M.;Korzekwa, K.R.;Crespi, C.L.;Gonzalez, F.J.;Gelboin, H.V. ] / Mol. Carcinogen.
5	Effects of organosulfur compounds from garlic and onions on benzo[a]pyrene-induced neoplasia and glutathione S-transferase activity in the mouse / [ Sparnins, V.L.;Barany, G.;Wattenberg, L.W. ] / Carcinogenesis
6	Antitumor activity of diallyl sulfide on polycyclic aromatic hydrocarbon-induced mouse skin carcino-genesis / [ Singh, A.;Shukla, Y. ] / Cancer Lett.
7	Organosulfur compounds from alliaceae in the prevention of human pathologies / [ Tapiero, H.;Townsend, D.M.;Tew, K.D. ] / Biomed. Pharmacother.
8	Chemoprevention of colon carcinogenesis by organosulfur compounds / [ Reddy, B.S.;Chinthalapally, V.R.;Rivenson, A.;Kellof, G. ] / Cancer Res.
9	Antimicrobial activity of alk(en)yl sulfides found in essential oils of garlic and onion / [ Kim, J.W.;Huh, J.E.;Kyung, S.H.;Kyung, K.H. ] / Food Sci. Biotechnol.
10	Mechanism of differential efficacy of garlic organosulfides in preventing benzo[a]pyrene-induced cancer in mice / [ Srivastava, S.K.;Hu, X.;Xia, H.;Zaren, H.A.;Chatterjee, M.L.;Agarwal, R.;Singh, S.V. ] / Cancer Lett.
11	Modulation of cytochrome P450-mediated bioactivation of benzo[a]pyrene by volatile allyl sulfides in human hepatoma cells / [ Chun, H.S.;Kim, H.J.;Choi, E.H. ] / Biosci. Biotech. Biochem.
12	Noncompetitive mixed-type inhibition of rainbow trout CYPIA catalytic activity by clotrimazole / [ Levine, S.L.;Oris, J.T. ] / Comp. Biochem. Phys.
13	Expression of CYP1A1 in human adult and fetal tissues and differential inducibility of CYP1B1 and CYP1A1 and Ah receptor ligands in human placenta and cultured cells / [ Hakkola, J.;Pasanen, M.;Pelkonen, O.;Hukkanen, J.;Evisalmi, S.;Anttila, S.;Rane, A.;Mantyla, M.;Purkunen, R.;Saarikoski, S.;Tooming, M.;Raunio, H. ] / Carcinogenesis
14	Metabolism of 2-acetylaminofluorene and benzo[a]pyrene and activation of food-derived heterocyclic amine mutagens by human cytochrome P-450 / [ McManus, M.E.;Burgess, W.M.;Veronese, M.E.;Huggett, A.;Quattrochi, L.C.;Tukey, R.H. ] / Cancer Res.
15	2-Amino-3,4-dimethylimidazo[4,5-f]quinoline induces and inhibits cytochrome P450 from the 1A subfamily in chick and rat hepatocytes / [ Sinclair, J.F.;Schaeffer, B.K.;Wood, S.G.;Lambrecht, L.K.;Gorman, N.;Bement, W.J.;Smith, E.L.;Sinclair, P.R.;Waldren, C.A. ] / Cancer Res.
16	Use of a cyclodextrin for increased protective activity of volatile allyl sulfides against benzo[a] pyrene-induced toxicity in human cell model / [ Kim, H.J.;Chun, H.S.;Yang, R. ] / Biotechnol. Lett.
17	Analogues of aminoglutethimide: selective inhibition of cholesterol side-chain cleavage / [ Foster, A.B.;Jarman, M.;Leung, C.S.;Rowlands, M.G.;Taylor, G.N. ] / J. Med. Chem.
18	Enzyme and metabolic inhibitors / [ Webb, J.L. ] /
19	Inhibition of cytochrome P450 2E1 by diallyl sulfide and its metabolites / [ Brady, J.F.;Ishizaki, H.;Fukuto, J.M.;Lin, M.C.;Fadel, A.;Gapac, J.M.;Yang, C.S. ] / Chem. Res. Toxicol.