Browse > Article

Isolation of Hyaluronidase Inhibitory Component from the Roots of Astraglus membranaceus Bunge (Astragali Radix)  

Lee, Yun-Mi (Department of Biological Engineering, Inha University)
Choi, Soo-Im (Department of Biological Engineering, Inha University)
Lee, Jae-Won (Department of Biological Engineering, Inha University)
Jung, Sun-Mi (Department of Biological Engineering, Inha University)
Park, Sang-Min (Department of Biological Engineering, Inha University)
Heo, Tae-Ryeon (Department of Biological Engineering, Inha University)
Publication Information
Food Science and Biotechnology / v.14, no.2, 2005 , pp. 263-267 More about this Journal
Abstract
In order to isolate hyaluronidase (HAase) inhibitor from Astragali radix (AR), dried roots were extracted with ethanol, prior to sequential fractionations with n-hexane, chloroform, ethyl acetate, n-butanol, and aqueous fractions. The n-butanol soluble fraction was found to exhibit the most pronounced inhibitory effect (68%) on HAase, and the active components were separated using various chromatographic methods, including column chromatography and preparative HPLC. The active component was isolated from the n-butanol soluble fraction of AR and was structurally identified as calycosin-7-O-${\beta}$-D-glucopyranoside by LC-MS, IR, $^1H$ NMR, and $^{13}C$ NMR analysis. The $IC_{50}$ of calycosin-7-O-${\beta}$-D-glucopyranoside's HAase activity was found to be 3.7 mg/mL.
Keywords
Astragalus membranaceus Bunge; calycosin-7-O-${\beta}$-D-glucopyronoside; hyaluronidase inhibitor;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
연도 인용수 순위
1 Inhibitory effects of some natural products on the activation of hyaluronidase and their anti-allergic actions /
[ Kakegawa, H.;Matsumoto, H.;Satoh, T. ] / Chem. Pharm. Bull.   ScienceOn
2 Inhibitory effects of hydrangenol derivatives on the activities /
[ Kakegawa, H.;Matsumoto, H.;Satoh, T. ] / Planta Med.
3 Mineral Contents and anti-inflammatory effect of ostrich extract combined with Korean herb medicine /
[ Kim, E.J.;Yuh, C.S.;Lee, P.J.;Choi, M.K.;Kim, S.Y. ] / Food Sci. Biotechnol.
4 A convenient plate assay for the quantitation of hyaluronidase in Hymenoptera venoms /
[ Richman, P.G.;Baer, H. ] / Anal. Biochem.
5 Preparative isolation and purification of two isoflavones from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography /
[ Ma, X.;Tu, P.;Chen, Y.;Zhang, T.;Wei, T.;Ito, Y. ] / J. Chromatogr.
6 Lipid chromatography-electrospray ionization mass spectrometry study of the flavonoids of the roots of Astragalus mongholicus and A. membranaceus /
[ Lin, L.Z.;He, X.G.;Lindenmaier, M.;Nolan, G.;Yang, J.;Cleary, M.;Qiu, S.X.;Cordell, G.A. ] / J. Chromatogr.
7 Hyaluronidase inhibitor from Uncariae Ramulus et Uncus /
[ Jeong, S.J.;Ko, Y.S.;Ahn, N.H.;Kim, Y.C. ] / Korean J. Pharmacogn.
8 Effects of SKI 306X, a new herbal agent, on proteoglycan degradation in cartilage explant culture and collagenase-induced rabbit osteoarthritis model /
[ Choi, J.H.;Choi, J.H.;Kim, D.Y.;Yoon, J.H.;Youn, H.Y.;Yi, J.B.;Rhee, H.I.;Ryu, K.H.;Jung, K.;Han, C.K.;Kwak, W.J.;Cho, Y.B. ] / Osteoarthritis Cartilage
9 A study on the constituents from the roots of Astragalus membranaceus Bunge (<TEX>${\beta}$</TEX>) /
[ Kim, J.S.;Kim, Y.T.;Kim, C.S. ] / Korean J. Pharmacogn.
10 The hyaluronidase: A chemical, biological and clinical overview /
[ Feost, G.I.;Csoka, T.;Stern, R. ] / Trends Glycosci. Glycotech.   ScienceOn
11 Assay for hyaluronidase activity;Methods in Enzymology, Vol. 235 /
[ Hynes, W.L.;Ferretti, J.J. ] /
12 The effect of anti-inflammatory drugs on the enzyme activity of a rat liver granular fraction high increases vascular permeability /
[ Anderson, A.J. ] / Biochem. Pharmacol.
13 Ascorbic acid and cancer: a review /
[ Cameron, E.;Pauling, L.;Leibovitz, B. ] / Cancer Res.
14 Elevated plasma levels of hyaluronic acid in patients with osteoarthritis and rheumatoid arthritis /
[ Golberg, R.L.;Huff, J.P.;Lenz, M.E.;Glickman, P.;Katz, R.;Thonar, E.J.M.A. ] / Arth. Rheum.
15 Screening of hyaluronidase inhibitory and free radical scavenging activity in vitro of tmditional herbal ？medicine extracts /
[ Choi, S.I.;Lee, Y.M.;Heo, T.R. ] / Korean J. Biotechnol. Bioeng.
16 Inhibitory effects of hydrolysable tannins on <TEX>$Ca^{+2}$</TEX>-activated HAase /
[ Lee, J.;Lee, S.H.;Min, K.R.;Lee, K.S.;Ro, J.S.;Ryu, J.C.;Kim, Y. ] / Planta Med.
17 Hypotensive and antiinflammatory effects of Astragalus saponin 1 /
[ Zang, Y.D.;Wang, Y.L.;Shen, J.P.;Li, D.X. ] / Acta Pharm. Sin.
18 Inhibitory effect of Astragali Radix on COX-2 activity /
[ Kim, E.J.;Oh, O.J.;Lee, S.K.;Yang, K.S. ] / Korean J. Pharmacogn.
19 A study on the constituents from the roots of Astragalus memhranaceus Bunge (<TEX>${\beta}$</TEX>) /
[ Kim, J.S.;Kim, C.S. ] / Korean J. Pharmacogn.