[KSCI] Korea Science Citation Index Service

Purification and Identification of Squalene Synthase Inhibitor Isolated from Fermented Soybean Paste

Choi, Sung-Won (Green Biotech Co. Ltd.)
Kim, Dong-Seob (Department of Food Science, Milyang National University)
Hur, Nam-Youn (Department of Food and culinary, Osan College)
Park, Cheon-Seok (Institute of Life Sciences and Resources, Kyung Hee University)
Baik, Moo-Yeol (Institute of Life Sciences and Resources, Kyung Hee University)

Publication Information

Food Science and Biotechnology / v.14, no.1, 2005 , pp. 89-93 More about this Journal

Abstract

Squalene synthase (SQS) inhibitors were screened from various plants and food extracts. Effective SQS inhibitor was purified from fermented soybean paste using ethanol extraction, HP-20 column chromatography, ethyl acetate extraction, silica gel column chromatography, and crystallization. Through UV spectrometry, $^1H$ NMR, $^{13}C$ NMR, and mass spectrometry, SQS inhibitor was identified as daidzein with molecular mass of 254 and molecular formula of $C_{15}H_{10}O_4$ . Daidzein showed $IC_{50}$ value of 50 nmol/L against SQS, confirming its potential as therapeutic agent for hypercholesterolemia.

Keywords

squalene synthase inhibitor; daidzein; fermented soybean paste; hypercholesterolemia;

Citations & Related Records

Times Cited By KSCI : 1 (Citation Analysis)
Times Cited By Web Of Science : 3 (Related Records In Web of Science)

Reference
Cited By KSCI

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