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Ultrasonic Absorption Measurements of Bovine Serum Albumin Solutions in the Frequency Range 200 kHz to 3 MHz  

Bae, Jong-Rim (Department of Physics, Daegu University)
Publication Information
The Journal of the Acoustical Society of Korea / v.25, no.1E, 2006 , pp. 14-19 More about this Journal
Abstract
Ultrasonic absorption and velocity spectra in bovine serum albumin (BSA) aqueous solutions have been measured at $20^{\circ}C$ over the frequency range 0.2-3 MHz in the pH range 1.5-13.2. The high-Q ultrasonic resonator and pulse-echo overlap methods were used. At acid pH's, excess absorption over that of pH 7 was explained by double relaxation. The pH dependences of the relaxation frequency and maximum absorption per wavelength, showed that the relaxation at about 200 kHz was related to the expansion of molecules and that about 3 MHz resulted from the proton transfer reaction of carboxyl group. At alkaline pH's, the excess absorption was explained by double relaxation. The relaxation at about 300 kHz was associated with a helix-coil transition, and that about 3 MHz was attributed to the proton transfer reaction of phenolic group. The rate constants and volume changes associated with these processes were estimated.
Keywords
Relaxation Frequency; Maximum Absorption per Wavelength; Proton Transfer Reaction; Helix-Coil Transition;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
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