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http://dx.doi.org/10.4142/jvs.21202

First molecular detection of canine herpesvirus 1 (CaHV-1) in the Eastern Brazilian Amazon  

Castro, Marcela dos Santos (Laboratorio de Tecnologia Biomolecular, Instituto de Ciencias Biologicas, Universidade Federal do Para)
David, Maridelzira Betania Moraes (Hospital Veterinario Mario Dias Teixeira, Universidade Federal Rural da Amazonia)
Goncalves, Evonnildo Costa (Laboratorio de Tecnologia Biomolecular, Instituto de Ciencias Biologicas, Universidade Federal do Para)
Siqueira, Andrei Santos (Laboratorio de Tecnologia Biomolecular, Instituto de Ciencias Biologicas, Universidade Federal do Para)
Virgulino, Rodrigo Rodrigues (Laboratorio de Tecnologia Biomolecular, Instituto de Ciencias Biologicas, Universidade Federal do Para)
Aguiar, Delia Cristina Figueira (Laboratorio de Tecnologia Biomolecular, Instituto de Ciencias Biologicas, Universidade Federal do Para)
Publication Information
Journal of Veterinary Science / v.23, no.2, 2022 , pp. 18.1-18.10 More about this Journal
Abstract
Background: Canine herpesvirus type 1 (CaHV-1) infects dogs and is associated with neonatal deaths and reproductive, ocular, neurological, and respiratory problems. In Brazil, reports of CaHV-1 have been restricted to the southeast and south regions, particularly in municipalities in the state of Rio Grande do Sul. Objectives: To assess the presence and variability of CaHV-1 in canine populations in the state of Pará, North Brazil. Methods: Biological samples from 159 dogs from 4 municipalities in the State of Pará were evaluated using polymerase chain reaction and phylogenetic analyses, with the target being the viral enzyme, thymidine kinase. Results: CaHV-1 was detected in 13 dogs (8.2%), with 2 animals being from the municipality of Santa Bárbara do Pará, 8 from Algodoal Island, 2 from Salinópolis, and one from Capanema. The study sequences revealed 100% identity among themselves and 64% to 100% identity with the other nucleotide sequences from Australia, Brazil, United Kingdom, and United States, including 100% identity with the 2002 isolate from Australia. The 1996 isolate from France was grouped in a branch that was different from the sequence of this study. Conclusions: This study presents the first molecular detection of CaHV-1 in dogs from the Amazon region in northern Brazil. The nucleotide identity between the strains and cytosine insertion in the sequences isolated in this study suggests at least 2 strains of CaHV-1 circulating in Brazil (Pará and BTU-1).
Keywords
Canine herpesvirus; molecular diagnosis; epidemiology; the Amazon;
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