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http://dx.doi.org/10.4142/jvs.2021.22.e59

Investigation of morphological changes of HPS membrane caused by cecropin B through scanning electron microscopy and atomic force microscopy  

Hu, Han (National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology)
Jiang, Changsheng (State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University)
Zhang, Binzhou (State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University)
Guo, Nan (State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University)
Li, Zhonghua (State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University)
Guo, Xiaozhen (State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University)
Wang, Yang (National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology)
Liu, Binlei (National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology)
He, Qigai (State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University)
Publication Information
Journal of Veterinary Science / v.22, no.5, 2021 , pp. 59.1-59.13 More about this Journal
Abstract
Background: Antimicrobial peptides (AMPs) have been identified as promising compounds for consideration as novel antimicrobial agents. Objectives: This study analyzed the efficacy of cecropin B against Haemophilus parasuis isolates through scanning electron microscopy (SEM) and atomic force microscopy (AFM) experiments. Results: Cecropin B exhibited broad inhibition activity against 15 standard Haemophilus parasuis (HPS) strains and 5 of the clinical isolates had minimum inhibition concentrations (MICs) ranging from 2 to 16 ㎍/mL. Microelectrophoresis and hexadecane adsorption assays indicated that the more hydrophobic and the higher the isoelectric point (IEP) of the strain, the more sensitive it was to cecropin B. Through SEM, multiple blisters of various shapes and dents on the cell surface were observed. Protrusions and leakage were detected by AFM. Conclusions: Based on the results, cecropin B could inhibit HPS via a pore-forming mechanism by interacting with the cytoplasmic membrane of bacteria. Moreover, as cecropin B concentration increased, the bacteria membrane was more seriously damaged. Thus, cecropin B could be developed as an effective anti-HPS agent for use in clinical applications.
Keywords
Antimicrobial peptide; atomic force microscopy; scanning electron microscopy; Haemophilus parasuis;
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