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http://dx.doi.org/10.4142/jvs.2018.19.6.817

Comparison of immunoadjuvant activities of four bursal peptides combined with H9N2 avian influenza virus vaccine  

Zhang, Cong (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Zhou, Jiangfei (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Liu, Zhixin (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Liu, Yongqing (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Cai, Kairui (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Shen, Tengfei (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Liao, Chengshui (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Wang, Chen (Key Laboratory of Veterinary Biological Engineering, Henan University of Science and Technology)
Publication Information
Journal of Veterinary Science / v.19, no.6, 2018 , pp. 817-826 More about this Journal
Abstract
The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.
Keywords
adjuvants; bursal peptides-(I-IV); cellular immune response; humoral immune response; immune protection;
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