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http://dx.doi.org/10.4142/jvs.2007.8.1.65

Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE  

Shin, Eun-Kyung (South Branch, Gangwon Veterinary Service)
Seo, Yeon-Soo (School of Veterinary Medicine, Kangwon National University)
Han, Jeong-Hee (School of Veterinary Medicine, Kangwon National University)
Hahn, Tae-Wook (School of Veterinary Medicine, Kangwon National University)
Publication Information
Journal of Veterinary Science / v.8, no.1, 2007 , pp. 65-73 More about this Journal
Abstract
The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6 distinct fingerprint patterns, respectively. The most common identical RAPD pattern was produced by OPA- 07 which was shared by 32 isolates (71.1%), the pattern produced by OPA-08 was shared by 26 isolates (57.8%), and the pattern produced by OPA-18 was shared by 40 isolates (88.9%). The RAPD patterns of the vaccine strain and the 3 reference strains did not match any of the patterns produced by the field isolates when primers OPA-07 and OPA-08 were used. PFGE using the restriction endonuclease XbaI produced a total of 15 patterns consisting of 4 PFGE types (A, B, B1 and C, differing by ≥ 4 bands) and 11 A subtypes (differing by ≤ 3 bands). Most of the field isolates exhibited identical type A and B patterns, suggesting that they were related. The vaccine strain and the three reference strains showed different PFGE patterns as compared to the identical type A strains.
Keywords
Bordetella bronchiseptica; genetic diversity; PFGE; RAPD;
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1 Bemis DA, Burns EH. Bordetella. In: Gyles CL, Thoen CO (eds.). Pathogenesis of Bacterial Infections in Animals. 2nd ed. pp. 201-215, Iowa State University Press, Ames, 1993
2 Bemis DA, Greisen HA, Appel MJ. Bacteriological variation among Bordetella bronchiseptica isolates from dogs and other species. J Clin Microbiol 1977, 5, 471-480
3 Graham AC, Abruzzo GK. Occurrence and characterization of plasmids in field isolates of Bordetella bronchiseptica. Am J Vet Res 1982, 43, 1852-1855
4 Keil DJ, Fenwick B. Evaluation of canine Bordetella bronchiseptica isolates using randomly amplified polymorphic DNA fingerprinting and ribotyping. Vet Microbiol 1999, 66, 41-51   DOI   ScienceOn
5 Pedersen KB. The serology of Bordetella bronchiseptica isolated from pigs compared with strains from other animal species. Acta Pathol Microbiol Scand Suppl 1975, 83, 590- 594
6 Tynkkynen S, Satokari R, Saarela M, Mattila-Sandholm T, Saxelin M. Comparison of ribotyping, randomly amplified polymorphic DNA analysis, and pulsed-field gel electrophoresis in typing of Lactobacillus rhamnosus and L. casei strains. Appl Environ Microbiol 1999, 65, 3908-3914
7 Winstanley C, Shina A, Dawson S, Gaskell RM, Hart CA. Variation in Bordetella bronchiseptica flaA does not correlate with typing by macro-restriction analysis by pulsed-field gel electrophoresis. J Med Microbiol 2001, 50, 255-260   DOI
8 Yuk MH, Heininger U, Martinez de Tejada G, Miller JF. Human but not ovine isolates of Bordetella parapertussis are highly clonal as determined by PCR-based RAPD fingerprinting. Infection 1998, 26, 270-273   DOI   ScienceOn
9 Harris DL, Switzer WP. Turbinate atrophy in young pigs exposed to Bordetella bronchiseptica, Pasteurella multocida, and combined inoculum. Am J Vet Res 1968, 29, 777-785
10 Cowan ST, Steel KJ. Manual for the Identification of Medical Bacterial. 2nd ed. pp. 89-90, Cambridge University Press, London, 1974
11 Bemis DA, Plotkin BJ. Hemagglutination by Bordetella bronchiseptica. J Clin Microbiol 1982, 15, 1120-1127
12 Stull TL, LiPuma JJ, Edlind TD. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 1988, 157, 280-286   DOI   ScienceOn
13 Chang N, Chui L. A standardized protocol for the rapid preparation of bacterial DNA for pulsed-field gel electrophoresis. Diagn Microbiol Infect Dis 1998, 31, 275-279   DOI   ScienceOn
14 Gautom RK. Rapid pulsed-field gel electrophoresis protocol for typing of Escherichia coli O157:H7 and other gramnegative organisms in 1 day. J Clin Microbiol 1997, 35, 2977-2980
15 Peppler MS, Schrumpf ME. Phenotypic variation and modulation in Bordetella bronchiseptica. Infect Immun 1984, 44, 681-687
16 Khattak MN, Matthews RC. Genetic relatedness of Bordetella species as determined by macrorestriction digests resolved by pulsed-field gel electrophoresis. Int J Syst Bacteriol 1993, 43, 659-664   DOI   ScienceOn
17 Seo YS, Lee SH, Shin EK, Kim SJ, Jung R, Hahn TW. Pulsed-field gel electrophoresis genotyping of Salmonella gallinarum and comparison with random amplified polymorphic DNA. Vet Microbiol 2006, 115, 349-357   DOI   ScienceOn
18 Sacco RE, Register KB, Nordholm GE. Restriction endonuclease analysis discriminates Bordetella bronchiseptica isolates. J Clin Microbiol 2000, 38, 4387-4393
19 Barbier N, Saulnier P, Chachaty E, Dumontier S, Andremont A. Random amplified polymorphic DNA typing versus pulsed-field gel electrophoresis for epidemiological typing of vancomycin-resistant enterococci. J Clin Microbiol 1996, 34, 1096-1099
20 Ross RF, Switzer WP, Duncan JR. Comparison of pathogenicity of various isolates of Bordetella bronchiseptica in young pigs. Can J Comp Med Vet Sci 1967, 31, 53-57
21 Musser JM, Hewlett EL, Peppler MS, Selander RK. Genetic diversity and relationships in populations of Bordetella spp. J Bacteriol 1986, 166, 230-237   DOI
22 Zhang Y, Rajagopalan M, Brown BA, Wallace RJ Jr. Randomly amplified polymorphic DNA PCR for comparison of Mycobacterium abscessus strains from nosocomial outbreaks. J Clin Microbiol 1997, 35, 3132-3139
23 Rutter JM, Collings LA. The virulence of Bordetella bronchiseptica in atrophic rhinitis of pigs. In: Pedersen KB, Nielsen NC (eds.). Atrophic Rhinitis in Pigs. pp. 77-83, Commission of the European Communities, Luxembourg, 1983
24 Register KB, Boisvert A, Ackermann MR. Use of ribotyping to distinguish Bordetella bronchiseptica isolates. Int J Syst Bacteriol 1997, 47, 678-683   DOI   ScienceOn
25 Johnson R, Sneath PHA. Taxonomy of Bordetella and related organisms of the families Achromobacteraceae, Brucellaeae and Neisseriaceae. Int J Syst Bacteriol 1973, 23, 381-404   DOI
26 Moissenet D, Valcin M, Marchand V, Grimprel E, Begue P, Garbarg-Chenon A, Vu-Thien H. Comparative DNA analysis of Bordetella pertussis clinical isolates by pulsedfield gel electrophoresis, randomly amplified polymorphism DNA, and ERIC polymerase chain reaction. FEMS Microbiol Lett 1996, 143, 127-132   DOI
27 Giles CJ. Bordetellosis. In: Leman AD, Straw BE, Mengeling WL, D'Allaire S, Taylor DJ (eds.). Disease of Swine. 7th ed. pp. 436-445, Iowa State University Press, Ames, 1992
28 Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH, Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsedfield gel electrophoresis: Criteria for bacterial strain typing. J Clin Microbiol 1995, 33, 2233-2239
29 Vu-Thien H, Dulot C, Moissenet D, Fauroux B, Garbarg- Chenon A. Comparison of randomly amplified polymorphic DNA analysis and pulsed-field gel electrophoresis for typing of Moraxella catarrhalis strains. J Clin Microbiol 1999, 37, 450-452
30 Baysinger A. PRDC: is it new or deja vu? Pork '99 1999, 19, 64
31 Smith IM, Baskerville AJ. A selective medium facilitating the isolation and recognition the Bordetella bronchiseptica in pigs. Res Vet Sci 1979, 27, 187-192
32 Grimont F, Grimont PA. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol 1986, 137B, 165-175
33 Binns SH, Speakman AJ, Dawson S, Bennett M, Gaskell RM, Hart CA. The use of pulsed-field gel electrophoresis to examine the epidemiology of Bordetella bronchiseptica isolated from cats and other species. Epidemiol Infect 1998, 120, 201-208   DOI   ScienceOn