Browse > Article
http://dx.doi.org/10.4142/jvs.2007.8.1.15

Organotypic slice culture of the hypothalamic paraventricular nucleus of rat  

Cho, Eun-Seong (Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University)
Lee, So-Yeong (Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University)
Park, Jae-Yong (Department of Physiology, Institute of Health Science and Medical Research Center for Neural Dysfunction, Gyeongsang National University College of Medicine)
Hong, Seong-Geun (Department of Physiology, Institute of Health Science and Medical Research Center for Neural Dysfunction, Gyeongsang National University College of Medicine)
Ryu, Pan-Dong (Laboratory of Veterinary Pharmacology, BK21 Program for Veterinary Science and College of Veterinary Medicine, Seoul National University)
Publication Information
Journal of Veterinary Science / v.8, no.1, 2007 , pp. 15-20 More about this Journal
Abstract
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.
Keywords
electrophysiology; green fluorescent protein; immunocytochemistry; organotypic slice culture; paraventricular nucleus;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
연도 인용수 순위
1 Arima H, House SB, Gainer H, Aguilera G. Neuronal activity is required for the circadian rhythm of vasopressin gene transcription in the suprachiasmatic nucleus in vitro. Endocrinology 2002, 143, 4165-4171   DOI   ScienceOn
2 Belenky M, Wagner S, Yarom Y, Matzner H, Cohen S, Castel M. The suprachiasmatic nucleus in stationary organotypic culture. Neuroscience 1996, 70, 127-143   DOI   ScienceOn
3 Benediktsson AM, Schachtele SJ, Green SH, Dailey ME. Ballistic labeling and dynamic imaging of astrocytes in organotypic hippocampal slice cultures. J Neurosci Methods 2005, 141, 41-53   DOI   ScienceOn
4 Fields RL, House SB, Gainer H. Regulatory domains in the intergenic region of the oxytocin and vasopressin genes that control their hypothalamus-specific expression in vitro. J Neurosci 2003, 23, 7801-7809   DOI
5 Gahwiler BH. Organotypic monolayer cultures of nervous tissue. J Neurosci Methods 1981, 4, 329-342   DOI   ScienceOn
6 Keir SD, House SB, Li J, Xiao X, Gainer H. Gene transfer into hypothalamic organotypic cultures using an adenoassociated virus vector. Exp Neurol 1999, 160, 313-316   DOI   ScienceOn
7 Ueta Y, Fujihara H, Serino R, Dayanithi G, Ozawa H, Matsuda K, Kawata M, Yamada J, Ueno S, Fukuda A, Murphy D. Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons and isolated nerve terminals of the rat. Endocrinology 2005, 146, 406-413   DOI   ScienceOn
8 Xiang Z, Hrabetova S, Moskowitz SI, Casaccia-Bonnefil P, Young SR, Nimmrich VC, Tiedge H, Einheber S, Karnup S, Bianchi R, Bergold PJ. Long-term maintenance of mature hippocampal slices in vitro. J Neurosci Methods 2000, 98, 145-154   DOI   ScienceOn
9 Swanson LW, Sawchenko PE. Hypothalamic integration: organization of the paraventricular and supraoptic nuclei. Annu Rev Neurosci 1983, 6, 269-324   DOI   ScienceOn
10 Tasker JG, Dudek FE. Electrophysiological properties of neurones in the region of the paraventricular nucleus in slices of rat hypothalamus. J Physiol 1991, 434, 271-293   DOI
11 Bergold PJ, Casaccia-Bonnefil P. Preparation of organotypic hippocampal slice cultures using the membrane filter method. Methods Mol Biol 1997, 72, 15-22
12 Bartanusz V, Muller D, Gaillard RC, Streit P, Vutskits L, Kiss JZ. Local gamma-aminobutyric acid and glutamate circuit control of hypophyseotrophic corticotropin-releasing factor neuron activity in the paraventricular nucleus of the hypothalamus. Eur J Neurosci 2004, 19, 777-782   DOI   ScienceOn
13 Walker SJ, Vrana KE. Pituitary corticotroph function during the stress hyporesponsive period in neonatal rats. Neuroendocrinology 1993, 57, 1003-1010   DOI
14 Kuwahara S, Arima H, Banno R, Sato I, Kondo N, Oiso Y. Regulation of vasopressin gene expression by cAMP and glucocorticoids in parvocellular neurons of the paraventricular nucleus in rat hypothalamic organotypic cultures. J Neurosci 2003, 23, 10231-10237   DOI
15 Chong W, Li LH, Lee K, Lee MH, Park JB, Ryu PD. Subtypes of $\alpha_{1}- and \alpha_{2}$-adrenoceptors mediating noradrenergic modulation of spontaneous inhibitory postsynaptic currents in the hypothalamic paraventricular nucleus. J Neuroendocrinol 2004, 16, 450-457   DOI   ScienceOn
16 Kusano K, House SB, Gainer H. Effects of osmotic pressure and brain-derived neurotrophic factor on the survival of postnatal hypothalamic oxytocinergic and vasopressinergic neurons in dissociated cell culture. J Neuroendocrinol 1999, 11, 145-152   DOI   ScienceOn
17 House SB, Thomas A, Kusano K, Gainer H. Stationary organotypic cultures of oxytocin and vasopressin magnocellular neurones from rat and mouse hypothalamus. J Neuroendocrinol 1998, 10, 849-861   DOI   ScienceOn
18 Leutgeb JK, Frey JU, Behnisch T. LTP in cultured hippocampal-entorhinal cortex slices from young adult (P25- 30) rat. J Neurosci Methods 2003, 130, 19-32   DOI   ScienceOn
19 Stoppini L, Buchs PA, Muller D. A simple method for organotypic cultures of nervous tissue. J Neurosci Methods 1991, 37, 173-182   DOI   ScienceOn
20 Gahwiler BH, Capogna M, Debanne D, McKinney RA, Thompson SM. Organotypic slice cultures: a technique has come of age. Trends Neurosci 1997, 20, 471-477   DOI   ScienceOn
21 Graulich J, Hoffmann U, Maier RF, Ruscher K, Pomper JK, Ko HK, Gabriel S, Obladen M, Heinemann U. Acute neuronal injury after hypoxia is influenced by the reoxygenation mode in juvenile hippocampal slice cultures. Brain Res Dev Brain Res 2002, 137, 35-42   DOI   ScienceOn
22 Han SK, Chong W, Li LH, Lee IS, Murase K, Ryu PD. Noradrenaline excites and inhibits GABAergic transmission in parvocellular neurons of rat hypothalamic paraventricular nucleus. J Neurophysiol 2002, 87, 2287-2296   DOI
23 Bertini LT, Kursner C, Gaillard RC, Corder R, Kiss JZ. A tissue culture model of the hypophysiotrophic CRH producing neuronal system. Neuroendocrinology 1993, 57, 716-728   DOI
24 Hilton KJ, Bateson AN, King AE. A model of organotypic rat spinal slice culture and bolistic transfection to elucidate factors that drive the preprotachykinin-A promoter. Brain Res Brain Res Rev 2004, 46, 191-203   DOI   ScienceOn
25 Armstrong WE. Hypothalamic supraoptic and paraventricular nuclei. In: Paxinos G (ed.). The Rat Nervous System. 3rd ed. pp. 369-388, Elsevier Academic Press, San Diego, 2004