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http://dx.doi.org/10.3348/kjr.2014.15.4.411

Trastuzumab-Conjugated Liposome-Coated Fluorescent Magnetic Nanoparticles to Target Breast Cancer  

Jang, Mijung (Department of Radiology, Seoul National University Bundang Hospital)
Yoon, Young Il (Nanoimaging and Therapy Research Center, Institute of Nanoconvergence, Advanced Institutes of Convergence Technology, Seoul National University)
Kwon, Yong Soo (NanoBio Materials Chemistry Lab., Department of Applied Bioscience, CHA University)
Yoon, Tae-Jong (Nanoimaging and Therapy Research Center, Institute of Nanoconvergence, Advanced Institutes of Convergence Technology, Seoul National University)
Lee, Hak Jong (Department of Radiology, Seoul National University Bundang Hospital)
Hwang, Sung Il (Department of Radiology, Seoul National University Bundang Hospital)
Yun, Bo La (Department of Radiology, Seoul National University Bundang Hospital)
Kim, Sun Mi (Department of Radiology, Seoul National University Bundang Hospital)
Publication Information
Korean Journal of Radiology / v.15, no.4, 2014 , pp. 411-422 More about this Journal
Abstract
Objective: To synthesize mesoporous silica-core-shell magnetic nanoparticles (MNPs) encapsulated by liposomes (Lipo [MNP@m-$SiO_2$]) in order to enhance their stability, allow them to be used in any buffer solution, and to produce trastuzumab-conjugated (Lipo[MNP@m-$SiO_2$]-$Her2_{Ab}$) nanoparticles to be utilized in vitro for the targeting of breast cancer. Materials and Methods: The physiochemical characteristics of Lipo[MNP@m-$SiO_2$] were assessed in terms of size, morphological features, and in vitro safety. The multimodal imaging properties of the organic dye incorporated into Lipo[MNP@m-$SiO_2$] were assessed with both in vitro fluorescence and MR imaging. The specific targeting ability of trastuzumab (Her2/neu antibody, $Herceptin^{(R)}$)-conjugated Lipo[MNP@m-$SiO_2$] for Her2/neu-positive breast cancer cells was also evaluated with fluorescence and MR imaging. Results: We obtained uniformly-sized and evenly distributed Lipo[MNP@m-$SiO_2$] that demonstrated biological stability, while not disrupting cell viability. Her2/neu-positive breast cancer cell targeting by trastuzumab-conjugated Lipo[MNP@m-$SiO_2$] was observed by in vitro fluorescence and MR imaging. Conclusion: Trastuzumab-conjugated Lipo[MNP@m-$SiO_2$] is a potential treatment tool for targeted drug delivery in Her2/neu-positive breast cancer.
Keywords
Breast cancer; Drug delivery; Iron oxide nanoparticles; Magnetic resonance imaging; Trastuzumab;
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