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http://dx.doi.org/10.3348/kjr.2014.15.1.29

Sentinel Node Mapping of VX2 Carcinoma in Rabbit Thigh with CT Lymphography Using Ethiodized Oil  

Lee, Yoon Jin (Department of Radiology, Seoul National University Bundang Hospital)
Kim, Young Hoon (Department of Radiology, Seoul National University Bundang Hospital)
Lee, Kyoung Ho (Department of Radiology, Seoul National University Bundang Hospital)
Park, Ji Hoon (Department of Radiology, Seoul National University Bundang Hospital)
Lee, Hye Seung (Department of Pathology, Seoul National University Bundang Hospital)
Jung, Seung Chai (Department of Radiology, Seoul National University Hospital, Seoul National University College of Medicine)
Joo, Seung-Moon (Department of Radiology, Gangnam Severance Hospital, Yonsei University College of Medicine)
Publication Information
Korean Journal of Radiology / v.15, no.1, 2014 , pp. 29-36 More about this Journal
Abstract
Objective: To assess the feasibility of computed tomography (CT) lymphography using ethiodized oil for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh. Materials and Methods: This experiment received approval from the institutional animal use and care administrative advisory committee. Twenty-three rabbits with VX2 carcinoma in the thigh underwent CT before and after (1 hour, 2 hour) peritumoral injection of 2 mL ethiodized oil. After the CT examination, sentinel nodes were identified by peritumoral injection of methylene blue and subsequently removed. The retrieved sentinel and non-sentinel lymph nodes were investigated with radiographic and pathologic examinations. Based on the comparison of CT findings with those of radiographic and pathologic examinations, the diagnostic performance of CT for sentinel node identification was assessed. Results: All 23 rabbits showed 53 ethiodized oil retention nodes on post-injection CT and specimen radiography, and 52 methylene blue-stained nodes at the right femoroiliac area. Of the 52 blue-stained sentinel nodes, 50 nodes demonstrated ethiodized oil retention. Thus, the sentinel node detection rate of CT was 96% (50 of 52). On pathologic examination, 28 sentinel nodes in 17 rabbits (nodes/rabbit, mean ${\pm}$ standard deviation, $1.7{\pm}0.6$) harbored metastasis. Twenty seven of the 28 metastatic sentinel nodes were found to have ethiodized oil retention. Conclusion: Computed tomography lymphography using ethiodized oil may be feasible for sentinel node mapping in experimentally induced VX2 carcinoma in the rabbit thigh.
Keywords
Sentinel lymph node; Computed tomography; Lymphography; Lymphatic metastasis; Animal model;
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