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http://dx.doi.org/10.5423/PPJ.OA.09.2020.0173

Genetic Mapping of a Resistance Locus to Phytophthora sojae in the Korean Soybean Cultivar Daewon  

Jang, Ik-Hyun (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University)
Kang, In Jeong (Department of Central Area Crop Science, National Institute of Crop Science)
Kim, Ji-Min (Department of Crop Science and Biotechnology, College of Bioresource Science, Dankook University)
Kang, Sung-Taeg (Department of Crop Science and Biotechnology, College of Bioresource Science, Dankook University)
Jang, Young Eun (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University)
Lee, Sungwoo (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.36, no.6, 2020 , pp. 591-599 More about this Journal
Abstract
Phytophthora root and stem rot reduce soybean yields worldwide. The use of R-gene type resistance is currently crucial for protecting soybean production. The present study aimed to identify the genomic location of a gene conferring resistance to Phytophthora sojae isolate 2457 in the recombinant inbred line population developed by a cross of Daepung × Daewon. Singlemarker analysis identified 20 single nucleotide polymorphisms associated with resistance to the P. sojae isolate 2457, which explained ~67% of phenotypic variance. Daewon contributed a resistance allele for the locus. This region is a well-known location for Rps1 and Rps7. The present study is the first, however, to identify an Rps gene locus from a major soybean variety cultivated in South Korea. Linkage analysis also identified a 573 kb region on chromosome 3 with high significance (logarithm of odds = 13.7). This genomic region was not further narrowed down due to lack of recombinants within the interval. Based on the latest soybean genome, ten leucine-rich repeat coding genes and four serine/ threonine protein kinase-coding genes are annotated in this region, which all are well-known types of genes for conferring disease resistance in crops. These genes would be candidates for molecular characterization of the resistance in further studies. The identified R-gene locus would be useful in developing P. sojae resistant varieties in the future. The results of the present study provide foundational knowledge for researchers who are interested in soybean-P. sojae interaction.
Keywords
high-resolution genetic mapping; nucleotide-binding site-leucine rich repeat (NBS-LRR); Phytophthora sojae; plant resistance; soybean;
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