Browse > Article
http://dx.doi.org/10.5423/PPJ.NT.09.2019.0249

Identification of New Isolates of Phytophthora sojae and the Reactions of Korean Soybean Cultivars Following Hypocotyl Inoculation  

Kang, In Jeong (Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science, National Institute of Crop Science)
Kang, Sunjoo (Department of Crop Science, Chungnam National University)
Jang, Ik Hyun (Department of Crop Science, Chungnam National University)
Jang, Yun Woo (Department of Southern Area Crop Science, National Institute of Crop Science)
Shim, Hyung Kwon (Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science, National Institute of Crop Science)
Heu, Sunggi (Division of Crop Cultivation and Environment Research, Department of Central Area Crop Science, National Institute of Crop Science)
Lee, Sungwoo (Department of Crop Science, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.35, no.6, 2019 , pp. 698-704 More about this Journal
Abstract
Phytophthora root and stem rot (PRSR) caused by Phytophthora sojae is one of the most destructive diseases of soybean. PRSR recently became an issue as soybean cultivation in paddy fields increased in South Korea. The management of PRSR mainly involves R-gene-mediated resistance, however, little is known about the resistance in Korean cultivars. Major Korean soybean cultivars were investigated for the presence or absence of R-gene-mediated resistance to four P. sojae isolates, two of which were new isolates. Isolate-specific reactions were observed following P. sojae inoculation. Of 21 cultivars, 15-20 cultivars (71.4-95.2%) showed susceptible reaction for each isolate. Ten cultivars were susceptible to all the isolates, and six cultivars were identified to have R-gene-mediated resistance to one or two isolates. The results of this study would provide a framework for the discovery of resistant cultivars, development of new cultivars resistant to P. sojae, and investigation of pathogenic diversity of P. sojae population in South Korea.
Keywords
pathogenic diversity; Phytophthora root and stem rot; race-specific resistance; R-gene; soybean;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Zhang, S., Xu, P., Wu, J., Xue, A. G., Zhang, J., Li, W., Chen, C., Chen, W. and Lv, H. 2010. Races of Phytophthora sojae and their virulences on soybean cultivars in Heilongjiang, China. Plant Dis. 94:87-91.   DOI
2 Abney, T. S., Melgar, J. C., Richards, T. L., Scott, D. H., Grogan, J. and Young, J. 1997. New races of Phytophthora sojae with Rps1-d virulence. Plant Dis. 81:653-655.   DOI
3 Akamatsu, H., Kato, M., Ochi, S., Mimuro, G., Matsuoka, J.-I. and Takahashi, M. 2019. Variation in the resistance of Japanese soybean cultivars to Phytophthora root and stem rot during the early plant growth stages and the effects of a fungicide seed treatment. Plant Pathol. J. 35:219-233.   DOI
4 Allen, T. W., Bradley, C. A., Sisson, A. J., Byamukama, E., Chilvers, M. I., Coker, C. M., Collins, A. A., Damicone, J. P., Dorrance, A. E., Dufault, N. S., Esker, P. D., Faske, T. R., Giesler, L. J., Grybauskas, A. P., Hershman, D. E., Hollier, C. A., Isakeit, T., Jardine, D. J., Kelly, H. M., Kemerait, R. C., Kleczewski, N. M., Koenning, S. R., Kurle, J. E., Malvick, D. K., Markell, S. G., Mehl, H. L., Mueller, D. S., Mueller, J. D., Mulrooney, R. P., Nelson, B. D., Newman, M. A., Osborne, L., Overstreet, C., Padgett, G. B., Phipps, P. M., Price, P. P., Sikora, E. J., Smith, D. L., Spurlock, T. N., Tande, C. A., Tenuta, A. U., Wise, K. A. and Wrather, J. A. 2017. Soybean yield loss estimates due to diseases in the United States and Ontario, Canada, from 2010 to 2014. Plant Health Prog. 18:19-27.   DOI
5 Anderson, T. R. and Buzzell, R. I. 1992. Inheritance and linkage of the Rps7 gene for resistance to Phytophthora rot of soybean. Plant Dis. 76:958-959.   DOI
6 Athow, K. L. and Laviolette, F. A. 1982. Rps 6, a major gene for resistance to Phytophthora megasperma f. sp. glycinea in soybean. Phytopathology 72:1564-1567.   DOI
7 Athow, K. L., Laviolette, F. A., Mueller, E. H. and Wilcox, J. R. 1980. A new major gene for resistance to Phytophthoramegasperma var sojae in soybean. Phytopathology 70:977-980.   DOI
8 Bienapfl, J. C., Malvick, D. K. and Percich, J. A. 2011. Specific molecular detection of Phytophthora sojae using conventional and real-time PCR. Fungal Biol. 115:733-740.   DOI
9 Buzzell, R. I. and Anderson, T. R. 1992. Inheritance and race reaction of a new soybean Rps1 allele. Plant Dis. 76:600-601.   DOI
10 Burnham, K. D., Dorrance, A. E., Francis, D. M., Fioritto, R. J. and Martin, S. K. S. 2003. Rps8, a new locus in soybean for resistance to Phytophthora sojae. Crop Sci. 43:101-105.   DOI
11 Dorrance, A. and Grunwald, N. J. 2009. Phytophthora sojae: diversity among and within populations. In: Oomycete genetics and genomics: diversity, interactions, and research tools, eds. by K. Lamour and S. Kamoun, pp. 197-212. John Wiley & Sons, Inc., Hoboken, NJ, USA.
12 Cheng, Y., Ma, Q., Ren, H., Xia, Q., Song, E., Tan, Z., Li, S., Zhang, G. and Nian, H. 2017. Fine mapping of a Phytophthora-resistance gene RpsWY in soybean (Glycine max L.) by high-throughput genome-wide sequencing. Theor. Appl. Genet. 130:1041-1051.   DOI
13 Demirbas, A., Rector, B. G., Lohnes, D. G., Fioritto, R. J., Graef, G. L., Cregan, P. B., Shoemaker, R. C. and Specht, J. E. 2001. Simple sequence repeat markers linked to the soybean Rps genes for Phytophthora resistance. Crop Sci. 41:1220-1227.   DOI
14 Diers, B. W., Mansur, L., Imsande, J. and Shoemaker, R. C. 1992. Mapping Phytophthora resistance loci in soybean with restriction fragment length polymorphism markers. Crop Sci. 32:377-383.   DOI
15 Dorrance, A. E. 2018. Management of Phytophthora sojae of soybean: a review and future perspectives. Can. J. Plant Pathol. 40:210-219.   DOI
16 Dorrance, A. E., Berry, S. A., Anderson, T. R. and Meharg, C. 2008. Isolation, storage, pathotype characterization, and evaluation of resistance for Phytophthora sojae in soybean. Plant Health Prog. Online publication. DOI: 10.1094/php-2008-0118-01-dg.
17 Dorrance, A. E., Jia, H. and Abney, T. S. 2004. Evaluation of soybean differentials for their interaction with Phytophthora sojae. Plant Health Prog. Online publication. DOI: 10.1094/PHP-2004-0309-01-RS.
18 Flor, H. H. 1971. Current status of the gene-for-gene concept. Annu. Rev. Phytopathol. 9:275-296.   DOI
19 Gordon, S. G., Martin, S. K. S. and Dorrance, A. E. 2006. Rps8 maps to a resistance gene rich region on soybean molecular linkage group F. Crop Sci. 46:168-173.   DOI
20 Grau, C. R., Dorrance, A. E., Bond, J. and Russin, J. S. 2004. Fungal diseases. In: Soybeans: improvement, production, and uses, eds. by H. R. Boerma and J. E. Specht, pp. 679-763. American Society of Agronomy. Crop Science Society of America. Soil Science Society of America, Madison, WI, USA.
21 Huang, J., Guo, N., Li, Y., Sun, J., Hu, G., Zhang, H., Li, Y., Zhang, X., Zhao, J., Xing H. and Qiu, L. 2016. Phenotypic evaluation and genetic dissection of resistance to Phytophthora sojae in the Chinese soybean mini core collection. BMC Genet. 17:85.   DOI
22 Jee, H. J., Kim, W. G. and Cho, W. D. 1998. Occurrence of Phytophthora root rot on soybean (Glycine max) and identification of the causal fungus. Crop Prot. 40:16-22.   DOI
23 Kaufmann, M. J. and Gerdemann, J. W. 1958. Root and stem rot of soybean caused by Phytophthora sojae n. sp. Phytopathology 48:201-208.
24 Kilen, T. C., Hartwig, E. E. and Keeling, B. L. 1974. Inheritance of a second major gene for resistance to Phytophthora rot in soybeans. Crop Sci. 14:260-262.   DOI
25 Koenning, S. R. and Wrather, J. A. 2010. Suppression of soybean yield potential in the continental United States by plant diseases from 2006 to 2009. Plant Health Prog. Online publication. DOI: 10.1094/PHP-2010-1122-01-RS.
26 Sugimoto, T., Kato, M., Yoshida, S., Matsumoto, I., Kobayashi, T., Kaga, A., Hajika, M., Yamamoto, R., Watanabe, K., Aino, M., Matoh, T., Walker, D. R., Biggs, A. R. and Ishimoto, M. 2012. Pathogenic diversity of Phytophthora sojae and breeding strategies to develop Phytophthora-resistant soybeans. Breed. Sci. 61:511-522.   DOI
27 Sun, J., Li, L., Zhao, J., Huang, J., Yan, Q., Xing, H. and Guo, N. 2014. Genetic analysis and fine mapping of RpsJS, a novel resistance gene to Phytophthora sojae in soybean [Glycine max (L.) Merr.]. Theor. Appl. Genet. 127:913-919.   DOI
28 Wagner, R. E. and Bernard, R. L. 1991. Response of Williams isolines to eight races of Phytophthora megasperma f. sp. glycinea. Soybean Genet. Newsl. 18:236-239.
29 Li, Y., Sun, S., Zhong, C., Wang, X., Wu, X. and Zhu, Z. 2017. Genetic mapping and development of co-segregating markers of RpsQ, which provides resistance to Phytophthora sojae in soybean. Theor. Appl. Genet. 130:1223-1233.   DOI
30 Sun, S., Wu, X. L., Zhao, J. M., Wang, Y. C., Tang, Q. H., Yu, D. Y., Gai, J. Y. and Xing, H. 2011. Characterization and mapping of RpsYu25, a novel resistance gene to Phytophthora sojae. Plant Breed. 130:139-143.   DOI
31 Wang, Y., Zhang, W., Wang, Y. and Zheng, X. 2006. Rapid and sensitive detection of Phytophthora sojae in soil and infected soybeans by species-specific polymerase chain reaction assays. Phytopathology 96:1315-1321.   DOI
32 Weng, C., Yu, K., Anderson, T. R. and Poysa, V. 2001. Mapping genes conferring resistance to Phytophthora root rot of soybean, Rps1a and Rps7. J. Hered. 92:442-446.   DOI
33 Wrather, A. and Koenning, S. 2009. Effects of diseases on soybean yields in the United States 1996 to 2007. Plant Health Prog. Online publication. DOI: 10.1094/PHP-2009-0401-01-RS.
34 Zhang, J., Xia, C., Wang, X., Duan, C., Sun, S., Wu, X. and Zhu, Z. 2013. Genetic characterization and fine mapping of the novel Phytophthora resistance gene in a Chinese soybean cultivar. Theor. Appl. Genet. 126:1555-1561.   DOI
35 Mideros, S., Nita, M. and Dorrance, A. E. 2007. Characterization of components of partial resistance, Rps2, and root resistance to Phytophthora sojae in soybean. Phytopathology 97:655-662.   DOI
36 Mueller, E. H., Athow, K. L. and Laviolette, F. A. 1978. Inheritance of resistance to four physiologic races of Phytophthora megasperma var. sojae. Phytopathology 68:1318-1322.   DOI
37 Niu, J., Guo, N., Sun, J., Li, L., Cao, Y., Li, S., Huang, J., Zhao, J., Zhao, T. and Xing, H. 2017. Fine mapping of a resistance gene RpsHN that controls Phytophthora sojae using recombinant inbred lines and secondary populations. Front. Plant Sci. 8:538.
38 Sahoo, D. K., Abeysekara, N. S., Cianzio, S. R., Robertson, A. E. and Bhattacharyya, M. K. 2017. A novel Phytophthora sojae resistance Rps12 gene mapped to a genomic region that contains several Rps genes. PLoS ONE 12:e0169950.   DOI
39 Schmitthenner, A. F. 1985. Problems and progress in control of Phytophthora root rot of soybean. Plant Dis. 69:362-368.   DOI
40 Stewart, S. M. 2011. Characterization and management of genetic diversity in Phytophthora sojae populations. Ph.D. thesis. Iowa State University, Ames, IA, USA.
41 Su, Y. C. and Shen, C. Y. 1993. The discovery and biological characteristics studies of Phytophthora megasperma f. sp. glycinea on soybean in China. Acta Phytopathol. Sin. 23:341-347 (in Chinese).
42 Lin, F., Zhao, M., Ping, J., Johnson, A., Zhang, B., Abney, T. S., Hughes, T. J. and Ma, J. 2013. Molecular mapping of two genes conferring resistance to Phytophthora sojae in a soybean landrace PI 567139B. Theor. Appl. Genet. 126:2177-2185.   DOI