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http://dx.doi.org/10.5423/PPJ.NT.06.2018.0108

Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification  

Kim, Nam-Yeon (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Oh, Jonghee (School of Applied Biosciences, Kyungpook National University)
Lee, Su-Heon (School of Applied Biosciences, Kyungpook National University)
Kim, Hongsup (Seed Testing & Research Center, Korea Seed & Variety Service)
Moon, Jae Sun (Plant Genome Research Center, Korea Research Institute of Bioscience & Biotechnology)
Jeong, Rae-Dong (Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University)
Publication Information
The Plant Pathology Journal / v.34, no.6, 2018 , pp. 575-579 More about this Journal
Abstract
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as $4.7ng/{\mu}l$ of purified RNA obtained from an ASGV-infected plant. The major advantage of this assay is that the reaction for the target virus is completed in 1 min, and amplification only requires an incubation temperature of $42^{\circ}C$. This assay is a promising alternative method for pear breeding programs or virus-free certification laboratories.
Keywords
Apple stem grooving virus; molecular diagnosis; reverse transcription-recombinase polymerase amplification;
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