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http://dx.doi.org/10.5423/PPJ.NT.06.2018.0100

Characterization of the Lytic Bacteriophage phiEaP-8 Effective against Both Erwinia amylovora and Erwinia pyrifoliae Causing Severe Diseases in Apple and Pear  

Park, Jungkum (Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University)
Lee, Gyu Min (Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University)
Kim, Donghyuk (School of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST))
Park, Duck Hwan (Applied Biology Program, Division of Bioresource Sciences, Kangwon National University)
Oh, Chang-Sik (Department of Horticultural Biotechnology, College of Life Sciences, Kyung Hee University)
Publication Information
The Plant Pathology Journal / v.34, no.5, 2018 , pp. 445-450 More about this Journal
Abstract
Bacteriophages, bacteria-infecting viruses, have been recently reconsidered as a biological control tool for preventing bacterial pathogens. Erwinia amylovora and E. pyrifoliae cause fire blight and black shoot blight disease in apple and pear, respectively. In this study, the bacteriophage phiEaP-8 was isolated from apple orchard soil and could efficiently and specifically kill both E. amylovora and E. pyrifoliae. This bacteriophage belongs to the Podoviridae family. Whole genome analysis revealed that phiEaP-8 carries a 75,929 bp genomic DNA with 78 coding sequences and 5 tRNA genes. Genome comparison showed that phiEaP-8 has only 85% identity to known bacteriophages at the DNA level. PhiEaP-8 retained lytic activity up to $50^{\circ}C$, within a pH range from 5 to 10, and under 365 nm UV light. Based on these characteristics, the bacteriophage phiEaP-8 is novel and carries potential to control both E. amylovora and E. pyrifoliae in apple and pear.
Keywords
Bacteriophage; black shoot blight; Erwinia amylovora; Erwinia pyrifoliae; fire blight;
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