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http://dx.doi.org/10.5423/PPJ.NT.05.2017.0106

Generation of an Infectious Clone of a New Korean Isolate of Apple chlorotic leaf spot virus Driven by Dual 35S and T7 Promoters in a Versatile Binary Vector  

Kim, Ik-Hyun (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Han, Jae-Yeong (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Cho, In-Sook (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration)
Ju, HyeKyoung (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Moon, Jae Sun (Molecular Biofarming Research Center, Korea Research Institute of Bioscience & Biotechnology)
Seo, Eun-Young (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Kim, Hong Gi (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Hammond, John (United States Department of Agriculture - Agricultural Research Service, United States National Arboretum, Floral and Nursery Plants Research Unit)
Lim, Hyoun-Sub (Department of Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.33, no.6, 2017 , pp. 608-613 More about this Journal
Abstract
The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20 days post inoculation (dpi), but did not produce obvious symptoms. Nicotiana occidentalis and C. quinoa were inoculated through agroinfiltration. At 32 dpi the infection rate was evaluated; no C. quinoa plants were infected by agroinfiltration, but infection of N. occidentalis was obtained.
Keywords
agroinfiltration; Apple chlorotic leaf spot virus; full-length infectious cDNA clone; in vitro transcription; T7 promoter;
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1 Lee, G. P., Ryu, K. H., Kim, H. R., Kim, C. S., Lee, D. W., Kim, J. S., Park, M. H., Noh, Y. M., Choi, S. H., Han, D. H. and Lee, C. H. 2002. Cloning and phylogenetic characterization of coat protein genes of two isolates of Apple mosaic virus from 'Fuji' apple. Plant Pathol. J. 18:259-265.   DOI
2 Lee, G. S., Kim, J. H., Kim, H. R., Shin, I. S., Cho, K. H., Kim, S. H., Shin, S. H. and Kim, D. H. 2013. Production system of virus-free apple plants using heat treatment and shoot tip culture. Res. Plant Dis.19:288-293.   DOI
3 Lim, H. S., Vaira, A. M., Domier, L. L., Lee, S. C., Kim, H. G. and Hammond, J. 2010. Efficiency of VIGS and gene expression in a novel bipartite potexvirus vector delivery system as a function of strength of TGB1 silencing suppression. Virology 402:149-163.   DOI
4 Menzel, W., Jelkman, W. and Maiss, E. 2002. Detection of four apple viruses by multiplex RT-PCR assays with coamplification of plant mRNA as internal control. J. Virol. Methods 99:81-92.   DOI
5 Nemchinov, L., Hadidi, A., Candresse, T., Foster, J. A. and Verdervskaya, T. C. 1995. Sensitive detection of Apple chlorotic leaf spot virus from infected apple or peach tissue using RT-PCR, IC-RT-PCR, or multiplex IC-RT-PCR. Acta Hortic. 386:51-62.
6 Al Rwahnih, M., Turturo, C., Minafra, A., Saldarelli, P., Myrta, A., Pallas, V. and Savino, V. 2004. Molecular variability of Apple chlorotic leaf spot virus in different hosts and geographical regions. J. Plant Pathol. 86:117-122.
7 Goodin, M. M., Dietzgen, R. G., Schichnes, D., Ruzin, S. and Jackson, A. O. 2002. pGD vectors: versatile tools for the expression of green and red fluorescent protein fusions in agroinfiltrated plant leaves. Plant J. 31:375-383.   DOI
8 Cembali, T., Folwell, R. J., Wandschneider, P., Eastwell, K. C. and Howell, W. E. 2003. Economic implications of a virus prevention program in deciduous tree fruits in the US. Crop Prot. 22:1149-1156.   DOI
9 Cho, I. S., Igori, D., Lim, S. M., Choi, G. S., Hammond, J., Lmi , H. S. and Moon, J. S. 2016. Deep sequencing analysis of apple infecting viruses in Korea. Plant Pathol. J. 32:441-451.   DOI
10 German, S., Candresse, T., Lanneau, M., Huet, J. C., Pernollet, J. C. and Dunez, J. 1990. Nucleotide sequence and genomic organization of Apple chlorotic leaf spot closterovirus. Virology 179:104-112.   DOI
11 Guo, W., Zheng, W., Wang. M., Li, X., Ma, Y. and Dai, H. 2016. Genome sequences of three Apple chlorotic leaf spot virus isolates from hawthorns in China. PLoS One 11:e0161099.   DOI
12 Han, J. Y., Kim, J. K., Cheong, J. S., Seo, E. Y., Park, C. H., Ju, H. K., Cho, I. S., Gotoh, T., Moon, J. S., Hammond, J. and Lim, H. S. 2015. Survey of Apple chlorotic leaf spot virus and Apple stem grooving virus occurrence in Korea and frequency of mixed infections in apple. J. Fac. Agr. Kyushu Univ. 60:323-329.
13 Han, J. Y., Chung, J. S., Kim, J. K, Seo, E. Y., Kilcrease, J. P., Bauchan, G. R., Lim, S. M., Hammond, J. and Lim, H. S. 2016. Comparison of helper component-protease RNA silencing suppression activity, subcellular localization, and aggregation of three Korean isolates of Turnip mosaic virus. Virus Genes 52:592-596.   DOI
14 Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30:2725-2729.   DOI
15 Katsiani, A. T., Maliogka, V. I., Candresse, T. and Katis, N. I. 2014. Host-range studies, genetic diversity and evolutionary relationships of ACLSV isolates from ornamental, wild and cultivated Rosaceous species. Plant Pathol. 63:63-71.   DOI
16 Niu, F., Pan, S., Wu, Z., Jiang, D. and Li, S. 2012. Complete nucleotide sequences of the genomes of two isolates of Apple chlorotic leaf spot virus from peach (Prunus persica) in China. Arch. Virol. 157:783-786.   DOI
17 Park, C. H., Ju, H. K., Han, J. Y., Park, J. S., Kim, I. H., Seo, E. Y., Kim, J. K., Hammond, J. and Lim, H. S. 2017. Complete nucleotide sequences and construction of full-length infectious cDNA clones of cucumber green mottle mosaic virus (CGMMV) in a versatile newly developed binary vector including both 35S and T7 promoters. Virus Genes 53:286-299.   DOI
18 Sato, K., Yoshikawa, N. and Takahashi, T. 1993. Complete nucleotide sequence of the genome of an apple isolate of Apple chlorotic leaf spot virus. J. Gen. Virol. 74:1927-1931.   DOI
19 Satoh, H., Yoshikawa, N. and Takahashi, T. 1999. Construction and biolistic inoculation of an infectious cDNA clone of Apple chlorotic leaf spot trichovirus. Ann. Phytopathol. Soc. Jpn. 65:301-304.   DOI
20 Wu, Y. Q., Zhang, D. M., Chen, S. Y., Wang, X. F. and Wang, W. H. 1998. Comparison of three ELISA methods for the detection of Apple chlorotic leaf spot virus and Apple stem grooving virus. Acta Hortic. 472:55-60.
21 Youssef, F., Marais, A., Faure, C., Gentit, P. and Candresse, T. 2011. Strategies to facilitate the development of uncloned or cloned infectious full-length viral cDNAs: Apple chlorotic leaf spot virus as a case study. Virol. J. 8:488.   DOI