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http://dx.doi.org/10.5423/PPJ.NT.01.2017.0009

Characterization of a Begomovirus-Betasatellite Complex, Producing Defective Molecules in Spinach (Spinacia oleracea L.), a New Host for Begomovirus and Betasatellite Complex in Pakistan  

Tahir, Muhammad Nouman (Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering)
Hameed, Amir (Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering)
Amin, Imran (Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering)
Mansoor, Shahid (Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering)
Publication Information
The Plant Pathology Journal / v.33, no.5, 2017 , pp. 514-521 More about this Journal
Abstract
Spinach is a vegetable crop which is widely grown over a large area especially in Punjab province of Pakistan. Leaf curling and enations on spinach plant collected shown to be associated with the begomovirus Pedilanthus leaf curl virus (PeLCV) and Shahdadpur strain of Cotton leaf curl Multan betasatellite ($CLCuMB^{Sha}$). Defective molecules of half and quarter size derived from monopartite begomoviruses are usually generated by the deletion of virion-sense sequences. Characterization of defective molecules of PeLCV from spinach revealed that the molecules of half the size are derived from the deletion of complementary-sense genes while quarter size molecule appears to have evolved by further deletion. This is the first report of the begomovirus-betasatellite complex on spinach and unusual defective molecules derived from deletion of complementary-sense genes in Pakistan.
Keywords
begomovirus; cotton leaf curl multan betasatellite; Pedilanthus leaf curl virus; spinach;
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