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http://dx.doi.org/10.5423/PPJ.NT.02.2017.0039

Efficient Transmission and Propagation of Tomato Chlorosis Virus by Simple Single-Leaflet Grafting  

Lee, Huin (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kim, Mi-Kyeong (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
Kang, Jin-Ho (Department of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University)
Ju, Ho-Jong (Department of Agricultural Biology, Chonbuk National University)
Seo, Jang-Kyun (Department of International Agricultural Technology and Institutes of Green Bio Science and Technology, Seoul National University)
Publication Information
The Plant Pathology Journal / v.33, no.3, 2017 , pp. 345-349 More about this Journal
Abstract
Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient tomato seedlings. Forty-one of 46 tomato seedlings tested were grafted successfully with single-leaflets infected with ToCV. Among them, 36 seedlings (87.8%) were systemically infected with ToCV and developed typical symptoms. Our results demonstrated that single-leaflet grafting could provide a sufficient amount of inoculum for the transmission of ToCV to the grafted seedlings.
Keywords
graft inoculation; single-leaflet; tomato chlorosis virus;
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