Browse > Article
http://dx.doi.org/10.5423/PPJ.NT.10.2015.0224

Development of Specific Markers for Identification of Biovars 1 and 2 Strains of Pseudomonas syringae pv. actinidiae  

Lee, Young Sun (Department of Biology, Sunchon National University)
Kim, Gyoung Hee (Department of Plant Medicine, Sunchon National University)
Koh, Young Jin (Department of Plant Medicine, Sunchon National University)
Zhuang, Qiguo (Sichuan Provincial Academy of Natural Resources)
Jung, Jae Sung (Department of Biology, Sunchon National University)
Publication Information
The Plant Pathology Journal / v.32, no.2, 2016 , pp. 162-167 More about this Journal
Abstract
Pseudomonas syringae pv. actinidiae, the causal agent of canker in kiwifruit, can be divided into three biovars (biovars 1, 2, and 3). Strains belonging to biovar 1 produce phaseolotoxin and were isolated in Japan and Italy before 2008. Strains of biovar 2 produce coronatine instead of phaseolotoxin and have been isolated only in Korea. Strains belonging to biovar 3 produce neither phaseolotoxin nor coronatine and are responsible for the global outbreak of bacterial canker of kiwifruit in recent years. The biovar 3-specific primer set was developed in a previous work. In this study, two sets of PCR primers specific to strains of biovars 1 and 2, respectively, were developed based on random amplified polymorphic DNA analyses. Primers PsaJ-F and PsaJ-R produced a 481-bp region with genomic DNA of biovar 1 strains, whereas primers PsaK-F and PsaK-R amplified a 413-bp region present only in the genome of biovar 2 strains.
Keywords
bacterial canker; biovar; kiwifruit; molecular marker; Pseudomonas syringae pv. actinidiae;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Balestra, G. M., Renzi, M. and Mazzaglia, A. 2011. First report of Pseudomonas syringae pv. actinidiae on kiwifruit plants in Spain. New Dis. Rep. 24:10.   DOI
2 Balestra, G. M., Taratufolo, M. C., Vinatzer, B. A. and Mazzaglia, A. 2013. A multiplex PCR assay for detection of Pseudomonas syringae pv. actinidiae and differentiation of populations with different geographic origin. Plant Dis. 97:472-478.   DOI
3 Bastas, K. and Karakaya, A. 2012. First report of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae in Turkey. Plant Dis. 96:452.
4 Bereswill, S., Bugert, P., Volksch, B., Ullrich, M., Bender, C. L. and Geider, K. 1994. Identification and relatedness of coronatine-producing Pseudomonas syringae pathovars by PCR analysis and sequence determination of the amplification products. Appl. Environ. Microbiol. 60:2924-2930.
5 Chapman, J. R., Taylor, R. K., Weir, B. S., Romberg, M. K., Vanneste, J. L., Luck, J. and Alexander, B. J. R. 2012. Phylogenetic relationships among global populations of Pseudomonas syringae pv. actinidiae. Phytopathology 102:1034-1044.   DOI
6 Cunty, A., Poliakoff, F., Rivoal, C., Cesbron, S., Fischer-Le Saux, M., Lemaire, C., Jacques, M. A., Manceau, C. and Vanneste, J. L. 2015. Characterization of Pseudomonas syringae pv. actinidiae (Psa) isolated from France and assignment of Psa biovar 4 to a de novo pathovar: Pseudomonas syringae pv. actinidifoliorum pv. nov. Plant Pathol. 64:582-596.   DOI
7 European Plant Protection Organization, 2011. First report of Pseudomonas syringae pv. actinidiae in Chile. EPPO Reporting Service 3:55.
8 Koh, Y. J., Kim, G. H., Koh, H. S., Lee, Y. S., Kim, S.-C. and Jung, J. S. 2012. Occurrence of a new type of Pseudomonas syringae pv. actinidiae strain of bacterial canker on kiwifruit in Korea. Plant Pathol. J. 28:423-427.   DOI
9 Liang, Y., Zhang, X., Tian, C., Gao, A. and Wang, P. 2000. Pathogenic identification of kiwifruit bacterial canker in Shaanxi. J. Northwest Forestry College 15:37-39.
10 Mazzaglia, A., Renzi, M. and Balestra, G. M. 2011. Comparison and utilization of different PCR-based approaches for molecular typing of Pseudomonas syringae pv. actinidiae strains from Italy. Can. J. Plant Pathol. 33:8-18.   DOI
11 Mazzaglia, A., Studholme, D. J., Taratufolo, M. C., Cai, R., Almeida, N. F., Goodman, T., Guttman, D. S., Vinatzer, B. A. and Balestra, G. M. 2012. Pseudomonas syringae pv. actinidiae (PSA) isolates from recent bacterial canker of kiwifruit outbreaks belong to the same genetic lineage. PLoS One 7:e36518.   DOI
12 McCann, H. C., Rikkerink, E. H. A., Bertels, F., Fiers, M., Lu, A., Rees-George, J., Andersen, M. T., Gleave, A. P., Haubold, B., Wohlers, M. W., Guttman, D. S., Wang, P. W., Straub, C., Vanneste, J., Rainey, P. B. and Templeton, M. D. 2013. Genomic analysis of the kiwifruit pathogen Pseudomonas syringae pv. actinidiae provides insights into the origins of an emergent plant disease. PLoS Pathogens 9: e1003503.   DOI
13 Rees-George, J., Vanneste, J. L., Cornish, D. A., Pushparajah, I. P. S., Yu, J., Templeton, M. D. and Everett, K. R. 2010. Detection of Pseudomonas syringae pv. actinidiae using polymerase chain reaction (PCR) primers based on the 16S-23S rDNA intertranscribed spacer region and comparison with PCR primers based on other gene regions. Plant Pathol. 59:453-464.   DOI
14 Han, H. S., Koh, Y. J., Hur, J.-S. and Jung, J. S. 2003. Identification and characterization of coronatine-producing Pseudomonas syringae pv. actinidiae. J. Microbiol. Biotechnol. 13:110-118.
15 Balestra, G. M., Renzi, M. and Mazzaglia, A. 2010. First report of bacterial canker of Actinidia deliciosa caused by Pseudomonas syringae pv. actinidiae in Portugal. New Dis. Rep. 22:10.   DOI
16 Everett, K. R., Taylor, R. K., Romberg, M. K., Rees-George, J., Fullerton, R. A., Vanneste, J. L. and Manning, M. A. 2011. First report of Pseudomonas syringae pv. actinidiae causing kiwifruit bacterial canker in New Zealand. Australas. Plant Dis. Notes 6:67-71.   DOI
17 Ferrante, P. and Scortichini, M. 2009. Identification of Pseudomonas syringae pv. actinidiae as causal agent of bacterial canker of yellow kiwifruit (Actinidia chinensis Planchon) in central Italy. J. Phytopathol. 157:768-770.   DOI
18 Ferrante, P. and Scortichini, M. 2010. Molecular and phenotypic features of Pseudomonas syringae pv. actinidiae isolated during recent epidemics of bacterial canker on kiwifruit (Actinidia chinensis) in central Italy. Plant Pathol. 59:954-962.   DOI
19 Ferrante, P. and Scortichini, M. 2015. Redefining the global populations of Pseudomonas syringae pv. actinidiae based on pathogenic, molecular and phenotypic characteristics. Plant Pathol. 64:51-62.   DOI
20 Koh, H. S., Kim, G. H., Lee, Y. S., Koh, Y. J. and Jung, J. S. 2014. Molecular characteristics of Pseudomonas syringae pv. actinidiae strains isolated in Korea and a multiplex PCR assay for haplotype differentiation. Plant Pathol. J. 30:96-101.   DOI
21 Koh, Y. J., Cha, B. J., Chung, H. J. and Lee, D. H. 1994. Outbreak and spread of bacterial canker in kiwifruit. Kor. J. Plant Pathol. 10:68-72.
22 Koh, Y. J., Kim, G. H., Jung, J. S., Lee, Y. S. and Hur, J. S. 2010. Outbreak of bacterial canker on Hort16A (Actinidia chinensis Planchon) caused by Pseudomonas syringae pv. actinidiae in Korea. N. Z. J. Crop Hortic. Sci. 38:275-282.   DOI
23 Takikawa, Y., Serizawa, S., Ichikawa, T., Tsuyumu, S. and Goto, M. 1989. Pseudomonas syringae pv. actinidiae sp. nov., the causal bacterium of canker in kiwifruit in Japan. Ann. Phytopathol. Soc. Japan 55:437-444.   DOI
24 Sawada, H., Takeuchi, T. and Matsuda, I. 1997. Comparative analysis of Pseudomonas syringae pv. actinidiae and pv. phaseolicola based on phaseolotoxin-resistant ornitine carbamoyltransferase gene (argK) and 16S-23S rRNA intergenic spacer sequences. Appl. Environ. Microbiol. 63:282-288.
25 Scotichini, M. 1994. Occurrence of Pseudomonas syringae pv. actinidiae on kiwifruit in Italy. Plant Pathol. 43:1035-1038.   DOI
26 Scotichini, M., Marcelletti, S., Ferrante, P., Petriccione, M. and Firrao, G. 2012. Pseudomonas syringae pv. actinidiae: a reemerging, multi-faceted, pandemic pathogen. Mol. Plant Pathol. 13:631-640.   DOI
27 Untergasser, A., Cutcutache, I., Koressaar, T., Ye, J., Faircloth, B., Remm, M. and Rozen, S. 2012. Primer3-new capabilities and interfaces. Nucleic Acids Res. 40:e115.   DOI
28 Vanneste, J. L., Poliakoff, F., Audusseau, C., Cornish, D. A., Pailard, S., Rivoal, C. and Yu, J. 2011. First report of Pseudomonas syringae pv. actinidiae, the causal agent of bacterial canker of kiwifruit in France. Plant Dis. 95:1311.
29 Young, J. M. 2012. Pseudomonas syringae pv. actinidiae in New Zealand. J. Plant Pathol. 94:S1.5-S1.10.