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http://dx.doi.org/10.5423/PPJ.NT.08.2015.0173

Establishment of a Simple and Rapid Gene Delivery System for Cucurbits by Using Engineered Zucchini Yellow Mosaic Virus  

Kang, Minji (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Seo, Jang Kyun (Crop Protection Division, National Academy of Agricultural Science)
Choi, Hoseong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Choi, Hong Soo (Crop Protection Division, National Academy of Agricultural Science)
Kim, Kook Hyung (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
Publication Information
The Plant Pathology Journal / v.32, no.1, 2016 , pp. 70-76 More about this Journal
Abstract
The infectious full-length cDNA clone of zucchini yellow mosaic virus (ZYMV) isolate PA (pZYMV-PA), which was isolated from pumpkin, was constructed by utilizing viral transcription and processing signals to produce infectious in vivo transcripts. Simple rub-inoculation of plasmid DNAs of pZYMV-PA was successful to cause infection of zucchini plants (Cucurbita pepo L.). We further engineered this infectious cDNA clone of ZYMV as a viral vector for systemic expression of heterologous proteins in cucurbits. We successfully expressed two reporter genes including gfp and bar in zucchini plants by simple rub-inoculation of plasmid DNAs of the ZYMV-based expression constructs. Our method of the ZYMV-based viral vector in association with the simple rub-inoculation provides an easy and rapid approach for introduction and evaluation of heterologous genes in cucurbits.
Keywords
gene delivery; infectious clone; ZYMV;
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