Browse > Article
http://dx.doi.org/10.5423/PPJ.NT.06.2015.0110

Loop-mediated Isothermal Amplification Assay to Rapidly Detect Wheat Streak Mosaic Virus in Quarantined Plants  

Lee, Siwon (Water Supply and Sewerage Research Division, National Institute of Environmental Research)
Kim, Jin-Ho (Department of Chemistry, College of Natural Science, Dankook University)
Choi, Ji-Young (Department of Chemistry, College of Natural Science, Dankook University)
Jang, Won-Cheoul (Department of Chemistry, College of Natural Science, Dankook University)
Publication Information
The Plant Pathology Journal / v.31, no.4, 2015 , pp. 438-440 More about this Journal
Abstract
We developed a loop-mediated isothermal amplification (LAMP) method to rapidly diagnose Wheat streak mosaic virus (WSMV) during quarantine inspections of imported wheat, corn, oats, and millet. The LAMP method was developed as a plant quarantine inspection method for the first time, and its simplicity, quickness, specificity and sensitivity were verified compared to current reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR quarantine methods. We were able to quickly screen for WSMV at quarantine sites with many test samples; thus, this method is expected to contribute to plant quarantine inspections.
Keywords
LAMP; loop-mediated isothermal amplification; quarantine; wheat streak mosaic virus; WSMV;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Ahn, Y. C., Nam, Y. H., Park, S. N., Cho, M. H., Seo, J. W., Yoon, I. K., Park, Y. H. and Jang, W. C. 2008. Detection of Mycobacterium tuberculosis by loop-mediated isothermal amplification assay. J. Korean Chem. Soc. 52:273-280.   DOI
2 Animal, Plant and Fisheries Quarantine and Inspection Agency. 2013. List of plant quarantine viruses in Korea newly revised in 2013. Res. Plant Dis. 19:67-75.
3 Cho, M. H., Jang, W. C. and Choi, J. G. 2013. Detection for methicillin resistant Staphylococcus aureus in using bio-chip based loop mediated isothermal amplification assay. J. Korean Chem. Soc. 57:81-87.   DOI
4 French, R. and Robertson, N. L. 1994. An RT-PCR method for the detection of the wheat streak mosaic virus. J. Virol. Methods 49:93-100.   DOI
5 Lee, S. 2013. A study of molecular biological detection methods for seed-transmitted viruses in quarantine. Ph.D. thesis. Dankook University, Cheonan, Chungcheongnam-do, Korea.
6 Lee, S., Cha, M., Kim, S. M., Heo, N. Y., Shin, Y. G. and Lee, S. H. 2014. Development of nucleotide primers for diagnostic RT-PCR and nested PCR detection of three seed-transmitted viruses (CRLV, SpLV and WClMV) in quarantine. J. Agric. Life Sci. 48:75-83.
7 Lee, S., Kang, E. H., Chu, Y. M., Shin, Y. G. and Ahn, T. Y. 2013a. Development of PCR diagnosis system for plant quarantine seed-borne Wheat streak mosaic virus. Korean J. Microbiol. 49:112-117.   DOI
8 Lee, S., Kang, E. H., Heo, N. Y., Kim, S. M., Kim, Y. J. and Shin, Y. G. 2013b. Detection of Carnation necrotic fleck vrus and Carnation ringspot virus using RT-PCR. Res. Plant Dis. 19:36-44.   DOI
9 Lee, S., Kang, E. H., Shin, Y. G. and Lee, S. H. 2013c. Development of RT-PCR and nested PCR for detection of four quarantine plant viruses belonging to Nepovirus. Res. Plant Dis. 19:220-225.   DOI
10 Lee, S., Lee, J. Y., Shin, Y. G., Lee, S. H. and Ahn, T. Y. 2015. Development and verification of nested PCR assay for detection of Tobacco rattle virus in plant quarantine. J. Bacteriol. Virol. 45:54-61.   DOI
11 Lee, S. and Shin, Y. G. 2014. Development and practical use of RTPCR for seed-transmitted Prune dwarf virus in quarantine. Plant Pathol. J. 30:178-182.   DOI
12 Shin, Y. G. and Rho, J. Y. 2014. Development of a PCR diagnostic system for Iris yellow spot tospovirus in quarantine. Plant Pathol. J. 30:440-444.   DOI