Browse > Article
http://dx.doi.org/10.5423/PPJ.OA.02.2012.0031

Evaluation of Watermelon Germplasm for Resistance to Phytophthora Blight Caused by Phytophthora capsici  

Kim, Min-Jeong (Organic Agriculture Division, National Academy of Agricultural Science)
Shim, Chang-Ki (Organic Agriculture Division, National Academy of Agricultural Science)
Kim, Yong-Ki (Organic Agriculture Division, National Academy of Agricultural Science)
Jee, Hyeong-Jin (Organic Agriculture Division, National Academy of Agricultural Science)
Hong, Sung-Jun (Organic Agriculture Division, National Academy of Agricultural Science)
Park, Jong-Ho (Organic Agriculture Division, National Academy of Agricultural Science)
Han, Eun-Jung (Organic Agriculture Division, National Academy of Agricultural Science)
Publication Information
The Plant Pathology Journal / v.29, no.1, 2013 , pp. 87-92 More about this Journal
Abstract
This study was conducted to determine the Phytophthora rot resistance of 514 accessions of watermelon germplasm, Citrullus lanatus var lanatus. About 46% of the 514 accessions tested were collections from Uzbekistan, Turkey, China, U.S.A., and Ukraine. Phytophthora capsici was inoculated to 45-day-old watermelon seedlings by drenching with 5 ml of sporangial suspension ($10^6$ sporangia/ml). At 7 days after inoculation, 21 accessions showed no disease symptoms while 291 accessions of susceptible watermelon germplasm showed more than 60.1% disease severity. A total of 510 accessions of watermelon germplasm showed significant disease symptoms and were rated as susceptible to highly susceptible 35 days after inoculation. The highly susceptible watermelon germplasm exhibited white fungal hyphae on the lesion or damping off with water-soaked and browning symptoms. One accession (IT032840) showed moderate resistance and two accessions (IT185446 and IT187904) were resistant to P. capsici. Results suggest that these two resistant germplasm can be used as a rootstock and as a source of resistance in breeding resistant watermelon varieties against Phytophthora.
Keywords
Citrullus lanatus var lanatus; Phytophthora resistance; sporangia;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Munger, H. M., More, T. A. and Awni, S. 1984. A preliminary report on screening watermelon for resistance to watermelon mosaic virus 1 and 2. Cucurbit Genet. Coop. Rep. 7:61-62.
2 National Watermelon Promotion Board, Production Statistics. 2005. [Online]. (http://www.watermelon.org/watermelon_statistics. asp.[3 March 2009].
3 Noh, J. J., Lee, K. K., Kim, G. C., Kim, W., Ko, B. R., Choi, J. S. and Choi, Y. G. 2004. Changes in density of Phytophthora capsici Leonian and incidence of Phytophthora root and fruit rot in field-grown watermelon on different repeated cropping years. Kor. J. Hort. Sci. Technol. 22:49.
4 Norton, J. D., Cosper, R. D., Smith, D. A. and Rymal, K. S. 1986. 'AU-Jubilant' and 'AU-Producer' watermelon. Hort Sci. 21:1460-1461.
5 Pavón, C. F., Babadoost, M. and Lambert, K. N. 2008. Quantification of Phytophthora capsici oospores in soil by sieving-centrifugation and real-time polymerase chain reaction. Plant Dis. 92:143-119.   DOI   ScienceOn
6 Robinson, R. W. and Decker-Walters, D. S. 1997. Cucurbits. CAB Int. University Press, Oxon (GB) 226 pp.
7 Rubatzky, V. E. 2001. Origin, distribution and uses. In: Maynard D. N. (ed) Watermelons: Characteristics, production and marketing. ASHS Press, Alexandria, VA., pp. 21-26.
8 Song, V., Gusmini, G. and Wehner, T. C. 2004. Screening the watermelon germplasm collection for resistance to gummy stem blight. Acta Hort. 637:63-68.
9 Sowell, G. 1975. An additional source of resistance to gummy stem blight in watermelon. Plant Dis. Rep. 59:43-415.
10 Tanksley, S. D. 1983. Molecular markers in plant breeding. Plant Mol. Biol. Rep:3-8.
11 Taylor, M. J. 2002. Trends in World Cucurbit Production, 1991 to 2001. ASHS Press, Alexandria, VA, pp. 373-379.
12 Tian, D. and Babadoost, M. 2004. Host range of Phytophthora capsici from pumpkin and pathogenicity of isolates. Plant Dis. 88:485-489.   DOI   ScienceOn
13 Wehner, T. C. and Shetty, N. V. 2000. Screening the cucumber germplasm collection for resistance to gummy stem blight in North Carolina field tests. Hort Sci. 35:1132-1140.
14 Hojo, H., Pavan, M. A. and Silva, N. 1991. Aggressiveness of Papaya ringspot virus-watermelon strain on watermelon cultivars. Summa Phytopathologia 17:188–194.
15 Huh, Y. C., Solmaz, I. and Sari, N. 2008. Morphological characterization of Korean and Turkish watermelon germplasm. In: Pitrat, M. (ed) Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae, INRA, Avignon, France, pp. 399-406.
16 Islam, S. Z. and Babadoost, M. 2004. Evaluation of selected fungicides for control of Phytophthora blight of processing pumpkin. Fung. & Nemat. Tests 59:V129.
17 Jang, S. W., Kim, H. D., Yi, E. S. and Rho, Y. T. 2008. Incidence of Phytophthora rot caused by Phytophthora capsici Leon. in squash field and cultivar resistance. Kor. J. Hort. Sci. Technol. 26:484-489.
18 Jee, H. J., Cho, W. D. and Kim, C. H. 2000. Phytophthora diseases in Korea. Rural Development Administration, Suwon, Korea. pp. 226.
19 Jeffrey, C. 1975. Further notes on Cucurbitaceae: III. Some African taxa. Kew Bul. 30:475-493.   DOI
20 Kim, B. S. 1995. Fruit and vine rot of watermelon caused by Phytophthora capsici. Korean J. Plant Pathol. 11:98-99.
21 Kim, B. S. and Shon, E. Y. 1991. Fruit and stem rot of watermelon caused by Phytophthora drechsleri Tucker. Korean J. Plant Pathol. 7:48-51.
22 Kim, C. H. and Kim, Y. K. 2002. Present status of soil-borne disease incidence and scheme for its integrated management in Korea. Res. Plant Dis. 8:156-161.
23 King, S.R., Davis, A.R., Liu, W. and Levi, A. 2008. Grafting for disease resistance. HortScience. 43:1673-1676.
24 Marr, C. W. and Tisserat, N. 1998. Commercial vegetable production: Watermelon. Kansas State University Agricultural Experiment Station and Cooperative Extension Service. MF-1107.
25 Lee, J. M., Choi, G. W. and Janick, J. 2007. Horticulture in Korea. Kor. Soc. Hort. Sci. pp. 56-61.
26 Levi, A., Thomas, C.E., Wehner, T. C. and Keinath, A. P. 2001a. Genetic diversity among watermelon (Citrullus lanatus and Citrullus colocynthis) accessions. Genetic Resources and Crop Evolution. Kluwer Academic Publishers, Netherlands 48: 559-566.   DOI   ScienceOn
27 Levi, A., Thomas, C. E., Wehner, T. C. and Zhang, X. 2001b. Low genetic diversity indicates the need to broaden the genetic base of cultivated watermelon. Amer. J. Hort. Sci. 36:1096-1101.
28 Miles, C. 2004. Icebox watermelons. In: Crop Production, Vegetable Research and Extension. Washingston State University. Vancouver Research and Extension Center, Vancouver, USA. (http://www.wsu.edu/watermelons. Date accessed: 18 July 2007).
29 Moose, S. P. and Mumm, R. H. 2008. Molecular plant breeding as the foundation for 21st century crop improvement. Plant Physiol. 147:969-977.   DOI   ScienceOn
30 Morrissey, B. 2006. The Vineline, 7-10.
31 Babadoost, M. 2000. Outbreak of Phytophthora foliar blight and fruit rot in processing pumpkin fields in Illinois. Plant Dis. 84:1345.
32 Babadoost, M. 2004. Phytophthora blight: A serious threat to cucurbit industries (http://www.apsnet.org/publications/ apsnetfeatures/Pages/PhytophthoraBlight.aspx).
33 Babadoost, M., Tian, D., Islam, S. Z. and Pavon, C. 2008. Challenges and options in managing Phytophthora blight (Phytophthora capsici) of cucurbits. In: M. Pitrat, (ed) Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA meeting on genetics and breeding of Cucurbitaceae, INRA, Avignon, France, pp. 399-406.
34 Gunner, N., Pesic-Van Esbroeck, Z. and Wehner, T. 2004. Inheritance of resistance to the Watermelon strain of Papaya ringspot virus in Watermelon. HortScience 39:1175–1182.
35 Davis, A. R., Levi, A., Tettech, A., Wehner, T., Russo, V. and Pitrat, M. 2007. Evaluation of watermelon and related species for resistance to race 1W powdery mildew. J. Amer. Soc. For Hort. Sci. 132:790-795.
36 Erwin, D. C. and Ribeiro, O. K. 1996. Phytophthora Disease Worldwide. APS Press. St. Paul, MN 562 pp.
37 Gubler, W. D. and Davis, R. M. 1996. Phytophthora Root and Crown Rot. In: Compendium of Cucurbit Diseases, T. A. Zitter, D. L. Hopkins, and C. E. Thomas, (eds). APS Press, St. Paul, MN. pp. 19-20.
38 Gusmini, G., Song, R. H. and Wehner, T. C. 2005. New sources of resistance to gummy stem blight in watermelon. Crop Sci. 45:582-588.   DOI   ScienceOn
39 Hassell, R. L. and Hausbeck, M. 2004. Phytophthora lessons learned: Irrigation water and snap beans. The Vegetable Growers News 38:2829.
40 Hausbeck, M. K. and Lamour, K. H. 2004. Phytophthora capsici on vegetable crops: research progress and management challenges. Plant Dis. 88:1292-1303.   DOI   ScienceOn
41 Wehner, T. C., Shetty, N. V. and Elmstrom, G. W. 2001. Breeding and seed production. In: Maynard, D. N. (ed.). Watermelons: Characteristics, production, and marketing. ASHS Press, Alexandria, Va. p. 27-73.
42 Zhang, Y. P., Kyle, M., Anagnostou, K. and Zitter, T. A. 1997. Screening melon (Cucumis melo) for resistance to gummy stem blight in the greenhouse and field. Hort. Sci. 32:117-121.
43 Zohary, D. and Hopf, M. 2000. Domestication of Plants in the Old World, 3rd ed. Oxford University Press, p. 193.