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http://dx.doi.org/10.5423/PPJ.2012.28.2.185

In vitro Biofumigation of Brassica Tissues Against Potato Stem Rot Caused by Sclerotinia sclerotiorum  

Ojaghian, Mohammad Reza (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University)
Jiang, Heng (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University)
Xie, Guan-Lin (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University)
Cui, Zhou-Qi (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University)
Zhang, Jingze (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University)
Li, Bin (State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University)
Publication Information
The Plant Pathology Journal / v.28, no.2, 2012 , pp. 185-190 More about this Journal
Abstract
Sclerotinia sclerotiorum is a serious pathogen which causes yield loss in many dicotyledonous crops including potato. The objective of this study was to assess the potential of biofumigation using three Brassica crops including Brassica napus, B. juncea and B. campestris against potato stem rot caused by S. sclerotiorum by in vitro tests. Both macerated and irradiated dried tissues were able to reduce radial growth and sclerotia formation of five pathogen isolates on PDA, but macerated live tissues were more effective. Compared with other tested crops, B. juncea showed more inhibitory effect against the pathogen. The volatile compounds produced from macerated tissues were identified using a gas chromatograph-mass spectrometer. The main identified compounds were methyl, allyl and butyl isothiocyanates. Different concentrations of these compounds inhibited mycelial growth of the pathogen in vitro when applied as the vapor of pure chemicals. A negative relationship was observed between chemicals concentrations and growth inhibition percentage. In this study, it became clear that the tissues of local Brassica crops release glucosinolates and have a good potential to be used against the pathogen in field examinations.
Keywords
dual culture; glucosinolates; ion trap detector; volatile compounds;
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1 Van-Eylen, D., Oey, I., Hendrick, M. and Van-Loey, A. 2008. Behavior of mustard seed Sinapis alba L. myrosinase during temperature. Eur. Food Res. Technol. 226:545-553.   DOI   ScienceOn
2 Walker, J. C., Morell, S. and Foster, H. H. 1937. Toxicity of mustard oils and related sulfur compounds to certain fungi. Am. J. Bot. 24:536-541.   DOI   ScienceOn
3 Wu, B. M. and Subbarao, K. V. 2008. Effects of soil temperature, moisture, and burial depths on carpogenic germination of Sclerotinia sclerotiorum and S. minor. Phytopathology 98:1144-1152.   DOI   ScienceOn
4 George, F., Bomford, M. and Vincelli, P. 2009. Screening Brassica species for glucosinolate content. J. Environ. Sci. Health: 311-316.
5 Gomez, K. A. and Gomez, A. A. 1984. Statistical Procedures for Agricultural Research, John Wiley and Sons, pp. 139-153. Singapore.
6 Kirkegaard, J. A., Wong, P. T. W. and Desmarchelier, J. M. 1996. In vitro suppression of fungal root pathogens of cereals by Brassica tissues. Plant Pathol. 45:593-603.   DOI   ScienceOn
7 McGregor, D. I., Mullin, W. J. and Fenwick, G. R. 1983. Analytical methodology for determining glucosinolate composition and content. J. Assoc. Anal. Chem. 66:825-849.
8 Kleinwachter, M. and Selmar, D. 2004. A novel approach for reliable activity determination of ascorbic acid depending myrosinases. J. Biochem. Biophys. Meth. 59:253-265.   DOI   ScienceOn
9 Larkin, R. P. and Griffin, T. S. 2007. Control of soilborne potato diseases using Brassica green manure. Crop Prot. 26:1067-1077.   DOI   ScienceOn
10 Li, X. and Kushad, M. M. 2005. Purification and characterization of myrosinase from horseradish Armoracia rusticana roots. Plant Physiol. Biochem. 43:503-511.   DOI   ScienceOn
11 Ojaghian, M. R. 2009. First report of Sclerotinia sclerotiorum on potato plants in Iran. Australas. Plant Dis. Notes 41:39-41.
12 Purdy, L. H. 1979. Sclerotinia sclerotiorum: history, diseases and symptomatology, host range, geographic distribution and impact. Phytopathology 69:875-880.   DOI
13 Rosa, E. A. S., Heaney, R. K. and Fenwick, G. R. 1997. Glucosinolates in crop plants. Hort. Rev. 19:99-215.
14 Smolinska, U. and Horbowicz, M. 1999. Fungicidal activity of volatiles from selected cruciferous plants against resting propagules of soil-borne fungal pathogens. J. Phytopathol. 147: 119-124.   DOI
15 Brennan, J. P. and Murray, G. M. 1988. Australian wheat diseases: Assessing their economic importance. Agr. Sci. 127:26-35.
16 Adams, P. B. 1975. Factors affecting survival of Sclerotinia sclerotiorum in soil. Plant Dis. Rep. 59:599-603.
17 Bending, G. D. and Lincoln, S. D. 1999. Characterization of volatile sulphur-containing compounds produced during decomposition of Brassica juncea tissues in soil. Soil Biol. Biochem. 31:695-703.   DOI   ScienceOn
18 Boydston, R. A. and Hang, H. A. 1995. Rapeseed (Brassica napus) green manure crop suppresses weeds in potato (Solanum tuberosum). Weed Technol. 9:669-675.
19 Bones, A. and Rossiter, J. T. 1996. The myrosinase-glucosinolate system, its organization and biochemistry. Physiol. Plantarum 97:194-208.   DOI
20 Brown, P. D. and Morra, M. J. 1997. Control of soil-borne plant pests using glucosinolate containing plants. Adv. Agron. 61: 167-231.   DOI
21 Buskov, S., Serra, B., Rosa, E., Sorense, H. and Sorensen, J. C. 2002. Effects of intact glucosinolates and products produced from glucosinolates in myrosinase-catalyzed hydrolysis on the potato cyst nematode (Globodera rostichiensis). J. Agric. Food Chem. 50:690-695.   DOI   ScienceOn
22 Charron, C. S. and Sams, C. E. 1999. Inhibition of Pythium ultimum and Rhizoctonia solani by shredded leaves of Brassica species. J. Am. Soc. Hort. Sci. 124:462-467.