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http://dx.doi.org/10.5423/PPJ.OA.07.2011.0126

Distribution and Antifungal Activity of Endophytic Fungi in Different Growth Stages of Chili Pepper (Capsicum annuum L.) in Korea  

Paul, Narayan Chandra (Department of Agricultural Biology, Chungnam National University)
Deng, Jian Xin (Department of Agricultural Biology, Chungnam National University)
Sang, Hyun-Kyu (Department of Agricultural Biology, Chungnam National University)
Choi, Young-Phil (Department of Agricultural Biology, Chungnam National University)
Yu, Seung-Hun (Department of Agricultural Biology, Chungnam National University)
Publication Information
The Plant Pathology Journal / v.28, no.1, 2012 , pp. 10-19 More about this Journal
Abstract
This study investigated the distribution of endophytic fungi obtained from the surface sterilized tissues of leaves, stems and roots of chili pepper ($Capsicum$ $annuum$ L.) plants in seedling, flowering and fruiting stages in Korea and their antifungal activity. A total of 481 isolates were recovered and were identified using molecular techniques. Based on rDNA ITS gene sequence and phylogenetic analysis, 21 fungal genera were characterized, belonging to 16 Ascomycota and 5 Basidiomycota. $Penicillium$ in seedling stage, $Fusarium$ in flowering stage, $Colletotrichum$ followed by $Fusarium$, $Alternaria$ and $Xylaria$ in fruiting stage was predominant and $Alternaria$, $Cladosporium$ and $Fusarium$ were common in all growth stages. Among 481 endophytes 90 phenotypes were evaluated for the antimicrobial activity against three major pathogens ($Phytophthora$ $capsici$, $Colletotrichum$ $acutatum$ and $Fusarium$ $oxysporum$) of chili pepper. Among them 16 isolates inhibited the growth of at least one test microorganisms. Three strains showed a broad spectrum antifungal activity and displayed strong inhibition against chili pepper pathogenic fungi.
Keywords
endophytic fungi; Capsicum annuum L.; molecular taxonomy; antifungal activity;
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