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http://dx.doi.org/10.5423/PPJ.2011.27.2.120

Identification of Alternaria alternata as a Causal Agent for Leaf Blight in Syringa Species  

Mmbaga, Margaret T. (Otis A. Floyd Nursery Research Center, School of Agriculture and Consumer, Tennessee State University)
Shi, Ainong (Syngenta Seeds Inc.)
Kim, Mee-Sook (Department of Forestry, Environment, and Systems, Kookmin University)
Publication Information
The Plant Pathology Journal / v.27, no.2, 2011 , pp. 120-127 More about this Journal
Abstract
While many isolates of Alternaria alternata are common saprophytes on trees and shrubs, this study clearly demonstrated that A. alternata is a primary pathogen in lilac (Syringa sp.), causing a leaf-blight that affects different Syringa species. Isolates of Alternaria sp. were collected from leaf blight samples of lilacs in the field. The internal transcribed spacer (ITS) region and morphological characterization were used to identify lilac blight pathogen. Based on 100% ITS nucleotide sequence identities to the Alternaria genus in the GenBank and morphological features, these isolates were identified as A. alternata. Disease symptoms were reproduced in lilac plants inoculated with A. alternata mycelial plugs and sprayed with a fungus-free culture filtrate, indicating that pathogenesis in lilac involves secondary metabolites or toxins. Diagnostic primers were developed to detect Alternaria sp. and A. alternata in lilac leaf blight based on ITS region and four known genes associated with pathogenesis in A. alternata: mixed-linked glucanase precursor, endopolygalacturonase, hsp70, and histone genes. The results from our study indicated A. alternata is a primary pathogen in lilac leaf blight, and these diagnostic primers can be used as a tool for the fast detection of A. alternata associated with lilac leaf blight.
Keywords
Alternaria alternata; lilac leaf blight; Syringa species; pathogen detection; toxins;
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1 Agarwal, A., Garg, G. K., Devi, S., Mishra, D. P. and Singh, U. S. 1997. Ultrastructural changes in Brassica leaves caused by Alternaria brassicae and destruxin B. J. Plant Biochem. Biotechnol. 6:25-28.   DOI   ScienceOn
2 Akimitsu, K. Peever, T. and Timmer, L. W. 2003. Molecular, ecological and evolutionary approaches to understanding Alternaria diseases of citrus. Mol. Plant Pathol. 4:435-446.   DOI   ScienceOn
3 Chase, A. R. 1998. Alternaria diseases of ornamentals. Western Connection Turf & Ornamentals 1(3) (www.westernfarmservice.com/newsletters/turf/alternaria.pdf.).
4 Ellis. M. B. 1971. Demataceous hyphomycetes. CAB International, Wallingford, Oxon, England. 608 pp.
5 Eshel, D., Ben-Arie, R., Dinoor, A. and Prusky, D. 2000. Resistance of gibberellin-treated persimmon fruit to Alternaria alternata arises from the reduced ability of the fungus to produce endo-1,4-${\beta}$-glucanase. Phytopathology 90:1256-1262.   DOI   ScienceOn
6 White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications, eds. by M. A. Innis, D. H. Gelfand, J. J. Sninsky and T. J. White, pp. 315-332. Academic Press, New York, NY, USA.
7 Sinclair, W. A., Lyon, H. and Johnson, W. T. 1987. Diseases of Trees and Shrubs. Cornell University Press, Ithaca, NY, USA. 575 pp.
8 Tewari, J. P. 1983. Cellular alterations in the blackspot of rapeseed caused by Alternaria brassicae. Phytopathology 73:831.
9 Thomma, B. P. H. J. 2003. Pathogen profile Alternaria spp.: from general saprophyte to specific parasite. Mol. Plant Pathol. 4:225-236.   DOI   ScienceOn
10 Walton, J. D. 2000. Horizontal gene transfer and the evolution of secondary metabolite gene clusters in fungi: a hypothesis. Fungal Genet. Biol. 30:167-171.   DOI   ScienceOn
11 Zur, G., Shimoni, E., Hallerman, E. and Kashi, Y. 2002. Detection of Alternaria fungal contamination in cereal grains by a polymerase chain reaction-based assay. J. Food Prot. 65:1433-1440.
12 Konstantinova, P., Bonants, P. J. M., van Gent-Pelzer, M. P. E., van der Zouwen, P. and van den Bulk, R. 2002. Development of specific primers for detection and identification of Alternaria spp. in carrot material by PCR and comparison with blotter and plating assays. Mycol. Res. 106:23-33.   DOI   ScienceOn
13 Loladze, A., Garland Campbell, K. A. and Chen, X. 2005. Development of a detached leaf assay for stripe rust resistance screening. Agronomy Abstracts. http://crops.confex.com/crops/2005am/techprogram/P3298.HTM
14 Park, D.-S., Sayler, R. J., Hong, Y. G., Nam, M. H. and Yang, Y. 2008. A method for inoculation and evaluation of rice sheath blight disease. Plant Dis. 92:1.
15 Masangkay, R. F., Mabbayad, M. O., Paulitz, T. C. and Watson, A. K. 1999. Host range of Alternaria alternata f.sp. sphenocleae causing leaf blight of Sphenoclea zeylanica. Can. J. Bot. 77:103-112.
16 Mmbaga, M. T. and Sheng, H. 1997. Evaluation of lilac (Syringa spp.) for multiple disease resistance to powdery mildew and bacterial blight in McMinnville, TN. Proc. South. Nurserymen's Assoc. Res. Conf. 42:512-518.
17 Mmbaga, M. T., Sauvé, R. J., Nnodu, E. and Zhou, S. 2005. Multiple disease resistance to powdery mildew, bacterial blight, and Alternaria blight in lilac (Syringa spp.). J. Arboriculture 31:1-9.
18 Pharmacia Diagnostics. 1992. M6 Alternaria alternata (A. tenius). http://www.immunocapinvitrosight.com/templates/Allergens.asp?id=2204
19 Pryor, B. M. and T.J. Michailides, T. J. 2002. Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology 92:406-416.   DOI   ScienceOn
20 Pschedt, J. W. and Moorman, G. W. 2001. Lilac diseases. In: Diseases of Woody Ornamentals and Trees in Nurseries, ed. by R. K. Jones and D. M. Benson, pp. 221-224. APS Press, St. Paul, MN. USA.
21 Rotem, J. 1994. The genus Alternaria: Biology, epidemiology and pathogenicity. The American Phytopathological Society, St. Paul MN, USA. 322 pp.
22 Eshel, D., Lichter, A., Dinoor, A. and Prusky, D. 2002a. Characterization of Alternaria alternata glucanase genes expressed during infection of resistant and susceptible persimmon fruits. Mol. Plant Pathol. 3:347-358.   DOI   ScienceOn
23 Jones, R. K. and Benson, D. M. 2001. Diseases of Woody Ornamentals and Trees in Nurseries. APS PRESS, the American Phytopathological Society, St. Paul, Minnesota. USA. 350 pp.
24 Eshel, D., Miyara, I., Ailing, T., Dinoor, A. and Prusky, D. 2002b. pH regulates endoglucanase expression and virulence of Alternaria alternata in persimmon fruit. Mol. Plant-Microbe Interact. 15:774-779.   DOI   ScienceOn
25 Gardner, J. M., Kono,Y. and Chandler J. L. 1986. Bioassay and host-selectivity of Alternaria citri: The major toxin specific to rough lemon plants. Phytochemistry 24:2861-2868.
26 Isshiki, A., Akimitsu, K., Yamamoto, M. and Yamamoto, H. 2001. Endopolygalacturonase is essential for citrus black rot caused by Alternaria citri but not brown spot caused by Alternaria alternata. Mol. Plant-Microbe Interact. 14:749-757.   DOI   ScienceOn
27 Johnson, R. D., Johnson, L., Kohmoto, K., Otani, H., Lane, C. R. and Kodama, M. 2000. A polymerase chain reaction-based method to specifically detect Alternaria alternata apple pathotype (A. mali), the causal agent of Alternaria blotch of apple. Phytopathology 90:973-976.   DOI   ScienceOn
28 Kohmoto, K., Scheffer, R. P. and Whiteside, J. O. 1979. Host selective toxins from Alternaria citri. Phytopathology, 69:667-671.   DOI
29 Kohmoto, K., Akimitsu, K. and Otani, H. 1991. Correlation of resistance and susceptibility of citrus to Alternaria alternata with sensitivity to host specific toxins. Phytopathology 81:719-722.   DOI
30 Kohmoto, K., Itoh, Y., Shimomura, N. Kondoh, Y., Otani, H., Kodama, M., Nishimura, S. and Nakatsuka, S. 1993. Isolation and biological activities of two host-specific toxins from tangerine pathotype Alternaria altanata. Phytopathology 83:495-502.   DOI