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http://dx.doi.org/10.5423/PPJ.2011.27.1.089

Mutation in clpxoo4158 Reduces Virulence and Resistance to Oxidative Stress in Xanthomonas oryzae pv. oryzae KACC10859  

Cho, Jung-Hee (Department of Plant Medicine, Chungbuk National University)
Jeong, Kyu-Sik (Variety Testing Division, Korea Seed & Variety Service)
Han, Jong-Woo (Environment-friendly Agriculture Research Division, Chungbuk ARES)
Kim, Woo-Jae (Honam Agricultural Research Insstitute, RDA)
Cha, Jae-Soon (Department of Plant Medicine, Chungbuk National University)
Publication Information
The Plant Pathology Journal / v.27, no.1, 2011 , pp. 89-92 More about this Journal
Abstract
Cyclic AMP receptor-like protein (Clp), is known to be a global transcriptional regulator for the expression of virulence factors in Xanthomonas campestris pv. campestris (Xcc). Sequence analysis showed that Xanthomonas oryzae pv. oryzae (Xoo) contains a gene that is strongly homologous to the Xcc clp. In order to determine the role of the Clp homolog in Xoo, a marker exchange mutant of $clp_{xoo4158}$ was generated. Virulence and virulence factors, such as the production of cellulase, xylanase, and extracellular polysaccharides (EPS) and swarming motility were significantly decreased in the $clp_{xoo4158}$ mutant. Moreover, the mutation caused the strain to be more sensitive to hydrogen peroxide and to over-produce siderophores. Complementation of the mutant restored the mutation-related phenotypes. Expression of $clp_{xoo4158}$, assessed by reverse-transcription realtime PCR and clp promoter activity, was significantly reduced in the rpfB, rpfF, rpfC, and rpfG mutants. These results suggest that the clp homolog, $clp_{xoo4158}$, is involved in the control of virulence and resistance against oxidative stress, and that expression of the gene is controlled by RpfC and RpfG through a diffusible signal factor (DSF) signal in Xanthomonas oryzae pv. oryzae KACC10859.
Keywords
bacterial blight; Clp; oxidative stress; rpf gene; Xanthomonas oryze pv. oryzae;
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