Browse > Article
http://dx.doi.org/10.5423/PPJ.2010.26.1.070

Screening of Rice Blast Resistance Genes from Aromatic Rice Germplasms with SNP Markers  

Kim, Jeong-Soon (National Agrobiodiversity Center, Rural Development Administration)
Ahn, Sang-Nag (Department of Agronomy, Chungnam National University)
Kim, Chung-Kon (National Agrobiodiversity Center, Rural Development Administration)
Shim, Chang-Ki (National Agrobiodiversity Center, Rural Development Administration)
Publication Information
The Plant Pathology Journal / v.26, no.1, 2010 , pp. 70-79 More about this Journal
Abstract
Rice blast is one of the serious devastating diseases. This study was carried out to determine the genetic diversities of blast resistance (R) genes form 86 accessions of aromatic rice with eight SNP markers, z4792, zt4792, z60510, zt6057, k6415, k6411, k39575 and t256, which showed the close-set linkage to 6 major genes, Piz, Piz-t, Pik, Pik-m, Pik-p, and Pit. Four accessions of indica type, Mayataung, Yekywin Yinkya Hmwe, Basmati9-93, and Basmati5854, showed the positive amplicons of six major genes. Among 86 accessions, 83 accessions were detected both or one of Piz and Piz-t genes. Seventy three accessions contained the Piz gene with z4792 marker. In addition, 30 and 71 accessions possessed Piz-t gene with zt4792 and zt6057 markers, respectively. Ten accessions showed the positive bands for the Piz-t gene with both zt4792 and zt6057 markers. Only one accession, Khau Nua Keo, was not amplified for both Piz and Piz-t gene. But japonica type, Gerdeh, possessed only Piz gene between Piz and Piz-t. Fifty two accessions showed the three of Pik multiple genes and Pit gene. Four accessions, Iari7447, Daebunhyangdo2, Shiyayuuine, and Basmati 6129 possessed a Pik-p gene. Especially, Pit gene on chromosome 1 was detected with t256 marker in all of 83 accessions, exception of A-2, one accession of japonica type.
Keywords
aromatic rice; Pi genes; rice blast resistance; SNP marker;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
1 Zhu, M. L., Wang, L. and Pan, Q. H. 2004. Identification and characterization of a new blast resistance gene located on rice chromosome 1 through linkage and differential analysis. Phytopathology 94:515-519.   DOI   ScienceOn
2 Tabien, R. E., Li, Z., Paterson, A. H., Marchetti, M. A., Stansel, J. W. and Pinson, S. R. M. 2000. Mapping of four major rice blast resistance genes from ‘Lemont’ and ‘Teqing’ and evaluation of their combinatorial effect for field resistance. Theor. Appl. Genet. 101:1215-1225.   DOI
3 Teng, P. S., Klein-debbinck, H. W. and Pinnschmidt, H. 1991. An analysis of the blast pathosystem to guide modeling and foresting In: Rice blast Modeling and Foresting, pp. 2-5. IRRI.
4 Valent, B. 1990. Rice blast as a model system for plant pathology. Phytopathology 80:33-36.   DOI
5 Valent, B. and Chumley, F. G. 1994. A virulence genes and mechanism of genetic instability in the rice blast fungus. In: Rice blast disease, eds. Zeigler, R. S., Leong, S. A. and Teng, P. S., eds. pp.111-134. CAB International, Wallingford.
6 Wang, G. L., Mackill, D. J., Bonman, J. M., McCouch, S. R., Champoux, M. C. and Nelson, R. J. 1994. RFLP mapping of genes conferring complete and partial resistance to blast in a durably resistant rice cultivar. Genetics 136:1421-1434.
7 Wu, J. Z., Mizuno, H., Hayshi-Tsugane, M., ItoY., Chiden, Y., Fujisawa, M., Katagiri, S., Saji, S., Yoshiki, S., Karasawa, W., Yoshihara, R., Hayashi, A., Hobayashi, H., Ito, K., Hamada, M., Okamoto, M., Ikeno, M., Ichikawa, Y., Katayose, Y., Yano, M., Matsumoto, T. and Sasaki, T. 2003. Physical maps and recombination frequency of six rice chromosomes. Plant J. 36:720-730.   DOI   ScienceOn
8 Xia, J. Q., Correll, J. C., Lee, F. N., Marchetti, M. A. and Rhoads, D. D. 1993. DNA fingerprinting to examine micro geographic variation in the Magnaporhe grisea (Pyricularia grisea) population in two rice fields in Arkansas, Phytopathology 83:1029-1035.   DOI
9 Yaegashi, H. 1994. Use of resistant varieties and disease control for paddy rice. Agric. Hortic. 69:149-154.
10 Moon, H. P., Choi, Y. G., Jeong, G. H., Hwang, G. H., Lee, J. H., Kim, M. G., Cho, S. Y., Jeon, B. T., Kim, J. I., Hwang, H. G., Choi, H. C., Park, R. G. and Kim, Y. S. 1998. A new variety, multi disease resistant and semi-dwarf aromatic rice cultivar “Hangmibyeo 2ho”. Agricultural Science Reports of RDA, Korea. 40:34-40.
11 Moon, H. P., Jung, K. K., Choi, Y. G., Lee, J. H., Hwang, H. G., Kim, M. K., Yang, S. J., Hwang, K. H., Song, M. T., Hong, H. C., Shin, Y. S., Kim, H. Y., Ryu, H. Y., Choi, H.C. and Chung, M. N. 2003. A new aromatic and glutinous rice cultivar “Seolhyangchalbyeo”. Korean J. Breed. Sci. 35:241-242.
12 Nagato, Y. and Yoshimura, A. 1998. Report of the committee on gene symbolization, nomenclature and linkage groups. RGN 15: 13-74.
13 Ou, S. H. 1979. Breeding rice of resistance to blast, a critical view. In: Proceeding of the Rice Blast Workshop, pp. 79-137. International Rice Research Institute, Manila, Philippines,
14 Ou, S. H. 1985. Rice disease, 2nd ed. Commonwealth Mycological Institute.
15 Pan, Q. H., Wang, L. and Tanisaka, T. 1999. A new blast resistance gene identified in the Indian native rice cultivar Aus373 through allelism and linkage tests. Plant Pathol. 48:288-293.   DOI   ScienceOn
16 Sallaud, C., Lorieux, M., Roumen, E., Tharreau, D., Berruyer, R., Svestasrani, p., Garsmeur, O., Ghesquiere, A. and Notteghem, J. L. 2003. Identification of five new blast resistance genes in the highly blast-resistant rice variety IR64 using a QTL mapping strategy. Theor. Appl. Genet. 106:794-803.   DOI
17 Singh, V. P., Khush, G. S. and Delaruz, N. 1997. Variability and quality indices in aromatic rice germplasm. IRRN 22:22.
18 Sirithunya, P., Tragoonrung, S., Vanavichit, A., Pa-In, N., Vongsaprom, C. and Toojinda, T. 2002. Quantitative trait loci associated with leaf and neck blast resistance in recombinant inbred line population of rice (Oryza sativa). DNA Research 9:79-88.   DOI
19 Li, Y. B., Wu, C. J., Jiang, G. H., Wang, L. Q. and He, Y. Q. 2007. Dynamic analyses of rice blast resistance for the assessment of genetic and environmental effects. Plant Breeding 126:541-547.   DOI   ScienceOn
20 Lee, F. N. 1994. Rice breeding programs, blast epidemics and blast management in the United States. In: Rice blast disease, ed. Ziegler, R. S. pp. 489-500. C.A.B. International Wallingford, U.K.
21 Liu, B., Zhang, S. H., Zhu, X. Y., Yang, Q. Y., Wu, S. Z., Mei, M. T., Mauleon, R., Leach, J., Mew, T. and Leung, H. 2004. Cadidate defense genes as predictors of quantitative blast resistance in rice. Mol. Plant Microbe. Interact. 17:1146-1152.   DOI   ScienceOn
22 Liu, X. Q., Wang, L., Chen, S., Lin, F. and Pan, Q. H. 2005. Genetic and physical mapping of Pi36(t), a novel rice blast resistance gene located on rice chromosome 8. Mol. Genet. Genomics DOI10.1007/s0048-005-0032-5.   DOI
23 Mackill, D. J. and Bonman, J. M. 1992. Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology 82:746-749.   DOI
24 McCouch, S. R., Sweney, M., Li, J., Jiang, H., Thoson, M., Septiningish, E., Edwards, J., Moncada, P., Xiao, J., Garris, A., Tai, T., Martinez, C., Thome, J., Sugiono, M., McClung,A., Yuan L. P. and Ahn, S. N. 2007. Through the genetic bottleneck: O. rufipogon as a source of trait enhancing alleles for O. sativa. Euphytica 154:317-339.   DOI
25 Melchinger, A. E. 1990. Use of molecular markers in breeding for oligogenic disease resistance. Plant Breed. 104:1-19.   DOI
26 Mohan, M., Nair, S., Bhagwat, A., Krishna, T. G., Tano, M., Bhatia, C. R. and Sasaki, T. 1997. Genome mapping, molecular markers and marker-assisted selection in crop plants. Mol. Breed. 3:87-103.   DOI   ScienceOn
27 Kim, J. S., Ahn, S. N., Kang, H. K., Cho, Y. H., Gwag, J. G. and Lee, S. Y. 2008b. Estimation of Physico-chemical Characteristics of domestic aroma rice and foreign aroma rice. Korean J. Crop Sci. 53:203-216.   과학기술학회마을
28 Hittalmani, S., Parco, A., Mew, T. V., Zeigler, R. S. and Huang, N. 2000. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor. Appl. Genet. 100:1121-1128.   DOI
29 Khush, G. S. 1981. Breeding rice for multiple disease and insect resistance. In: Rice improvement in China and other Asian countries. pp. 220-237. International Rice Research Institute, Los Banos, Philippines.
30 Kim, J. S., Ahn, S. N., Cho, Y. H., Gwag, J. G., Kim, T. S., Lee, J. R. and Lee, S. Y. 2008a. Estimation of agronomic characteristics of domestic aromatic rice germplasm and foreign aromatic rice germplasm in RDA Genebank, Korea. Korean J. Crop Sci. 53:261-272.   과학기술학회마을
31 Kiyosawa, S. 1968. Inheritance of blast-resistance in some Chinese rice varieties and their derivatives. Jpn. J. Breed. 18:183-204.   DOI
32 Kiyosawa, S. 1969. Inheritance of resistance of rice varieties to a Philippine fungus strain of Pyricularia oryzae. Jpn. J. Breed. 19:61-73.   DOI
33 Kiyosawa, S. 1978. Inheritance of blast-resistance in some rice varieties. Jpn. J. Breed. 28:287-296   DOI
34 Kiyosawa, S. and Nomura, K. 1988. Disease resistance in crop plants and mutational breeding. Gamma Field Symposia No. 27. Institute of Radiation breeding, NIAR, MAFF, Ibaraki, Japan.
35 Kiyosawa, S. 1981. Gene analysis for blast resistance. Oryza 18:196-203.
36 Gu, K., Yang, B., Tian, D., Wu, L., Wang, D., Sreekala, C., Yang, F., Chu, Z., Wang, G. L., White, F. F. and Yin, Z. 2005. R gene expression induced by type-III effecter triggers disease resistance in rice. Nature 435:1122-1125.   DOI   ScienceOn
37 Kwon, J. O. and Lee, S. G. 2002. Real-time micro-weather factors of growing field to the epidemics of rice blast. Res. Plant Dis. 8:199-206. (in Korean, English abstract).   DOI   ScienceOn
38 Choi, Y. G., Kim, M. K., Jung, K. H., Cho, S. Y., Moon, H. P., Jun B. T., Choi, H. C., Park, N. G., Kim, G. W., Hwang, K. H., Kim, Y. S., Park, R. K. and Cho, J. Y. 1995. An aromatic semidwarf lodging resistant rice variety “Hyangmibyeo1ho”. Agricultural Science Reports of RDA, Korea. 37:67-74.
39 Fukuoka, S. and Okuno, K. 2001. QTL analysis and mapping of Pi21, a recessive gene for field resistance to rice blast in Japanese upland rice. Theor. Appl. Genet. 103:185-190.   DOI
40 Ha, K. Y., Kim, Y. D., Lee, J. K., Shin, H. T., Noh, T. H., Choung, J. I., Shin, M S., Ko, J. K., Kim, B. K., Lee, Y. T., Lee, S. Y., Kim, H. S., Kang, H. J., Lee, K. S. and Lee, S. Y. 2003. A new aromatic and high-yielding Japonica rice cultivar “Mihyangbyeo”. Korean J. Breed. Sci. 35:251-252.
41 Ha, K. Y., Lee, J. K., Shin, H. T., Lee, S. Y., Yang, B. G., Kim, B. K., Joung, J. I., Kim, Y. D., Shin, M. S., Ko, J. K., Lee, K. S., Kim, C. H. and Cho, S. Y. 2006. A new aromatic and good grain quality Japonica rice variety “Hyangnambyeo”. Agricultural Science Reports of RDA, Korea 38:54-60.
42 Han, S. S., Ryu, J. D., Shim, H. S., Lee, S. W., Hong, Y. K. and Cha, K. H. 2001. Breakdown of resistant cultivars by new race KI-1117a and race distribution of rice blast fungus during 1999-2000 in Korea. Res. Plant Dis. 7:86-92 (in Korean, English summary).
43 Hayashi, K., Yoshida, H. and Ashikawa, I. 2006. Development of PCR-based allele specific and InDel marker sets for nine rice blast resistance genes. Theor. Appl. Genet. 113:251-260.   DOI
44 Berruyer, R., Adreit, H., Milazzo, J., Gaillard, S., Berger, A., Dioh, W., Lebrun, M. H. and Tharreau, D. 2003. Identification and fine mapping of Pi33, the rice resistance gene corresponding to the Magnaporthe grisea avirulence gene ACE1. Theor. Appl Genet. 107:1139-1147.   DOI
45 Hayashi, K., Hashimoto, N., Daigen, M. and Ashikawa, I. 2004. Development of PCR-based SNP markers for rice blast resistance genes at the Piz locus. Theor. Appl. Genet. 108:1212-220.   DOI
46 Ashikawa, I., Hayashi, N., Yamane, H., Kanamori, H., Wu, J., Mastsumoto, T. and Yano, M. 2008. Two adjacent nucleotidebinding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance. Genetics 180:2267-2276.   DOI   ScienceOn
47 Bagali, P. G., Hittalmani, S., Shashidhar, S. Y. and Shashidhar, H. E. 2000. Identification of DNA markers linked to partial resistance for blast disease in rice across four locations. In: Advances in Rice Blast Research, D. Tharreau, M. H. Lebrun, N. J. Talbot, and J. L. Notteghem, eds. pp. 34-44. Kluwer Academic Publishers, Dordrecht, Netherlands.
48 Bonman, J. M., Dedios, T. I. V. and Khin, M. M. 1986. Physiological specialization of Pyricularia ortzae in the Philippines. Plant Dis. 70:767-769.   DOI
49 Causse, M. A., Fulton, T. M., Cho, Y. G., Ahn, S. N., Chunwongse, J., Wu, K. S., Xiao, J. H., Yu, Z. H., Ronald, P. C., Harrington, S. E., Second, G., McCouch, S. and Tanksley, S.D. 1994. Saturated molecular map of the rice genome based on an interspecific backcross population. Genetics 138:1251-1274.
50 Chauhan, S., Farman, M. L., Zhang, H. B. and Leong, N. 2002. Genetic and physical mapping of a rice blast resistance locus, PiCO39(t), that corresponds to the avirulence gene AVR1-CO39 of Magnaporthe grisea. Mol. Genet. Genomics 267: 603-612.   DOI
51 Chen, M., Presting, G. and Barbazuk, W. B. 2002. An integrated physical and genetic map of the rice genome. Plant Cell 14:537-545.   DOI
52 Arif, M., Kousar, S., Bajwa, M. A., Arif, A. and Zapar, Y. 2005. Genetic diversity among rice genotypes of Pakistan through random amplified polymorphic DNA (RAPD) analysis. Pakistan Journal of Botany 37:585-592.
53 Cho, Y. C., Kwon, S. W., Choi, I. S., Lee, S. K., Jeon, J. S., Oh, M. K., Roh, J. H., Hwang, H. G., Yang, S. J. and Kim. Y. G. 2007. Identification of major blast resistance genes in Korean rice varieties (Oryza sativa L.) using molecular markers. J. Crop Sci. Biotech. 10:265-276.