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http://dx.doi.org/10.5423/PPJ.2009.25.4.303

Determination of Genetic Divergence Based on DNA Markers Amongst Monosporidial Strains Derived from Fungal Isolates of Karnal Bunt of Wheat  

Seneviratne, J.M. (Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology)
Gupta, Atul K. (Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology)
Pandey, Dinesh (Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology)
Sharma, Indu (Department of Genetics and Plant Breeding, Punjab Agricultural University)
Kumar, Anil (Department of Molecular Biology and Genetic Engineering, G.B. Pant University of Agriculture and Technology)
Publication Information
The Plant Pathology Journal / v.25, no.4, 2009 , pp. 303-316 More about this Journal
Abstract
Genetic variation among the base isolates and monosporidial strains derived from these isolates of Tilletia indica- the causal agent of Karnal bunt (KB) in wheat, was analyzed by morphological, growth behaviors and RAPD-ISSR based molecular polymorphism. Genetic make up of fungal cultures vary among each other. The magnitude of variation in KBPN group is less (narrow genetic base) when compared to the other groups KB3, KB9 and JK (broad genetic base) reflecting that variability is a genetically governed process. The generation of new variation with different growth characteristics is not a generalized feature and is totally dependant on the original genetic make-up of the base isolate generating new monosporidial strains. Thus, it can be concluded that monosporidial strains derived from mono-teliosporic isolate, consists of genetically heterogeneous population. The morphological and genetic variability further suggests that the variation in T. indica strains is predominantly derived through the genetic rearrangements through para sexual means.
Keywords
DNA polymorphism; fungal variability; genetic divergence; ISSR; RAPD;
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