Browse > Article
http://dx.doi.org/10.5423/PPJ.2009.25.3.294

Occurrence of Stem Canker on Rape Caused by Leptosphaeria biglobosa in Korea  

Hong, Sung-Kee (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA))
Kim, Wan-Gyu (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA))
Shin, Dong-Beom (Crop Environment Research Division, National Institute of Crop Science, RDA)
Choi, Hyo-Won (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA))
Lee, Young-Kee (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA))
Lee, Sang-Yeob (Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration (RDA))
Publication Information
The Plant Pathology Journal / v.25, no.3, 2009 , pp. 294-298 More about this Journal
Abstract
Stem canker symptoms were observed in a rape field in Muan, Korea during a disease survey in May 2006. A total of 15 isolates of Phoma sp. were obtained from the infected stems of the plant. All isolates were identified as Leptosphaeria biglobosa based on their morphological and cultural characteristics. The Korean isolates of L. biglobosa were assigned to 'brassicae' among six subclades of L. biglobosa complex based on the entire ITS sequences of rDNA. Pathogenicity of the fungal isolates was confirmed on leaves and stems of rape by artificial inoculation. This is the first report that Leptosphaeria biglobosa causes stem canker of rape in Korea.
Keywords
Leptosphaeria biglobosa; pathogenicity; rape; stem canker;
Citations & Related Records

Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
  • Reference
1 Balesdent, M. H., Gall, C., Robin, P. and Rouxel, T. 1992. Intraspecific variation in soluble mycelial protein and esterase patterns of Leptosphaeria maculans French isolates. Mycol. Res. 96:677-684   DOI
2 Gall, C., Balesdent, M. H., Desthieux, I., Robin, P. and Rouxel, T. 1995. Polymorphism of $Tox^{0}$ Leptosphaeria maculans isolates as revealed by soluble protein and isozyme electrophoresis. Mycol. Res. 99:221-229   DOI   ScienceOn
3 Jedryczka, M., Rouxel, T. and Balesdent, M. H. 1999. Rep-PCR. based genomic fingerprinting of isolates of Leptosphaeria. maculans from Poland. Eur. J. Plant Pathol. 105:813-823   DOI   ScienceOn
4 Williams R. H. and Fitt, B. D. L. 1999. Differentiating A and B groups of. Leptosphaeria maculans, causal agent of stem canker (blackleg) of. oilseed rape. Plant Pathol. 48:161-175   DOI   ScienceOn
5 Fitt, B., Brun, H., Barbetti, M. and Rimmer, S. 2006. World-wide importance of phoma stem canker (Leptosphaeria maculans and L. biglobosa) on oilseed rape (Brassica napus). Eur. J. Plant Pathol. 114:3-15   DOI   ScienceOn
6 Voigt, K., Jedryczka, M. and Wostemeyer, J. 2001. Strain typing of Polish Leptosphaeria maculans isolates supports at the genonomic level the multispecies concept of aggressive and non-agressive strains. Microbial. Res. 156:169-177   DOI   ScienceOn
7 Koch, E., Song, K., Osborn, T. C. and Williams, P. H. 1991. Relationship between pathogenicity. and phylogeny based on restriction fragment length polymorphism in Leptosphaeria maculans. Mol. Plant-Microbe Intract. 4:341-349   DOI
8 Mendes-Pereira, E., Balesdent, M. H., Brun, H. and Rouxel, T. 2003. Molecular phylogeny of the Leptosphaeria maculans-L. biglobosa species complex. Mycol. Res. 107:1287-1304   DOI   ScienceOn
9 White, T. J., Bruns, T., Lee, S. and Taylor, J. W. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Pp. 315-322 In: PCR Protocols: A Guide to Methods and Applications. Academic press, New York, USA
10 Balesdent, M. H., Jedryczka, M., Jain, L., Mendes-Pereira, E., Bertrandy, J. and Rouxel, T. 1998. Conidia as a substrate for internal transcribed spacer-based PCR identification of members of the Leptosphaeria maculans species complex. Phytopathology 88:1210-1217   DOI   ScienceOn
11 Vincenot, L., Balesdent, M. H., Li, H., Barbetti, M. J., Sivasithamparam, K., Gout, L. and Rouxel, T. 2008. Occurrence of a new subclade of Leptosphaeria biglobosa in Western Australia. Phytopathology 98:321-329   DOI   ScienceOn
12 West, J. S., Kharbanda, P. D., Barbetti, M. J. and Fitt, B. D. L. 2001. Epidemiology and management of Leptosphaeria maculans (Phoma stem. canker) on oilseed rape in Australia, Canada and Europe. Plant Pathol. 50:10-27   DOI
13 Boerema G. H., Gruyter, J., Noordeloos, M. E. and Hamers, M. E. C. 2004. Phoma identification manual. Differentiation of specific and intra-specific taxa in culture. CAB International, Wallingford, UK. 448 pp
14 Cho, W. D. and Shin, H. D. eds. 2004. List of plant diseases in Korea. 4th edition. The Korean Society of Plant Pathology. Suwon, Korea. 779 pp
15 Tamura, K., Dudley, J., Nei, M. and Kumar, S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. BioI. Evol. 24:1596-1599   DOI   ScienceOn
16 Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic. Acids Res. 22:4673-4680   DOI   ScienceOn
17 Shoemaker, R. A. and Brun, H. 2001. The teleomorph of the weakly aggressive segregate of Leptosphaeria maculans. Can. J. Bot. 79:412-419   DOI