Browse > Article
http://dx.doi.org/10.5423/PPJ.2009.25.3.241

Early Disease Development and Stem and Leaf Water Content in the Seedlings of Pinus koraiensis Inoculated with Pinewood Nematodes in a Greenhouse  

Woo, Kwan-Soo (Department of Forest Resources Development, Korea Forest Research Institute)
Yoon, Jun-Hyuek (Department of Forest Resources Development, Korea Forest Research Institute)
Publication Information
The Plant Pathology Journal / v.25, no.3, 2009 , pp. 241-246 More about this Journal
Abstract
Korean pine (Pinus koraiensis Sieb. et Zucc.), a five-needle pine, has recently been suffering pine wilt disease caused by non-native pinewood nematode, Bursaphelenchus xylophilus. Three-year-old Korean pine seedlings were inoculated with 10,000 pathogenic nematodes in a greenhouse to investigate disease development, water content and the density of nematodes in stems. Needle dehydration, xylem drying and pith browning started 20 days after inoculation (DAI). There were significant differences between seedlings inoculated with nematodes and control seedlings in the relative water content of stems and leaves at 20 and 30 DAI. At 60 DAI, all remaining seedlings inoculated with nematodes had died, but control seedlings all remained alive. The average number of nematodes recovered from stems of Korean pine dramatically increased from 10 to 20 DAI, and then decreased at the end of the experiment at 60 days. This study suggests that the relative water content of stems and leaves in current-year branches could be used as a useful physiological indicator for early diagnosis of pine wilt disease.
Keywords
Bursaphelenchus xylophilus; pathogenic nematodes; Pinus koraiensis; water content; xylem drying;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Asai, E. and Futai, K. 2005. Effects of inoculum density of pinewood nematode on the development of pine wilt disease in Japanese black pinc sccdlings pretreatcd with simulated acid rain. Forest Pathol. 35:135-144   DOI   ScienceOn
2 Critchfield, W. B. and Little, E. L. 1966. Geographic distribution of the pines of the world: U.S. Department of Agriculture Miscellaneous Publication 991, 97 pp
3 Ikeda, T. and Suzaki, T. 1984. Influence of pine-wood nematodes on hydraulic conductivity and water status in Pinus thunbergii. Jap. For. Soc. 66:412-420
4 Kuroda, K. 2008. Physiological incidences related to symptom development and wilting mechanism. In: Pine Wilt Disease (Zhao, B., Futai K., Sutherland, J. and Takeuchi, Y, eds), pp. 204-222. Springer
5 Lim, J. H. 1989. Studies on the ecological characteristics of natural stands in Pinus koraiensis. Korea University, PhD. thesis paper. 95 pp.
6 Southey, J. F. 1986. Laboratory methods for work with plant and soil nematodes, 6th edn. HMSO, London.
7 Togashi, K., Aida, K., Nakamura, K., Horikoshi, T. and Takahashi, F. 1997. Different development of pine wilt disease in artificially infected Pinus thunbergii seedlings potted together with different tree species. J. For. Res. 2:39-43   DOI
8 Wang, Y. Y., Li, H. Y., Shu, C. R. and Guo, Z. H. 2001. A study on difference of pH between healthy and pine wilt diseased woods of several pine species. Acta Phytopathol. Sinica 31:342-348
9 Kuroda, K., Ohira, M., Okamura, M. and Fujisawa, Y. 2007. Migration and population growth of the pine wood nematode (Bursaphelenchus xylophilus) related to the symptom development in the seedlings of Japanese black pine (Pinus thunbergii) families selected as resistant to pine wilt. J. Jpn. For. Soc. 89:241-248   DOI   ScienceOn
10 Kuroda, K. and Ito, S. 1992. Migration speed of pine wood nematode and activities of other microbes during the development of pine-wilt disease in Pinus thunbergii. J. Jpn. For. Soc. 74:383-389
11 Mamiya, Y. 1972. Reproduction of pine lethal wilting disease by the inoculation of young trees with Bursaphelenchus lignicoIus. Jpn. J. Nematol. 2:40-44
12 Shin, S. C. and Han, H. R. 2006. Current status on research and management of pine wilt disease in Korea. In: Proceedings of the international symposium on current status on research and management of pine wilt disease, Seoul, Korea, pp. 31-40
13 Enda, N. 1989. The status of pine wilting disease caused by Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle and its control in Korea. J. Korean For. Soc. 78:248-253
14 Hyun,. S. K. 1969. Intrinsic qualities, growth-potential, and adaptatIon of white pine in Asia (Pinus koraiensis and Pinus armandil). International Symposium on Biology and International Aspects of Rust Resistance of Forest Tree, Moscow, Idaho, U.S.A., August 17-24, 1969
15 Kwon, T. S., Lim, J. H., Sim, S. J., Kwon, Y. D., Son, S. K., Lee, K. Y., Kim, Y. T., Park, J. W., Shin, C. H., Ryu, S. B., Lee, C. K., Shin, S. C., Chung, Y. J. and Park, Y. S. 2006. Distribution patterns of Monochamus alternatus and M saltuarius (Coleoptera: Cerambycidae) in Korea. J. Korean For. Soc. 95: 543-550
16 Mamiya, Y. 1980. Inoculation of the first year pine (Pinus dens iflora) seedlings with Bursaphelenchus lignicolus and the histopathology of diseased seedlings. Jpn. J. For. Soc. 62:176-183
17 Ikeda, T. and Kiyohara, T. 1995. Water relations, xylem embolism and histological features of Pinus thunbergii inoculated with virulent or avirulent pine wood nematode, Bursaphelenchus xylophilus. J. Exp. Bot. 46:441-449   DOI   ScienceOn
18 Melakeberhan, H., ToIvonen, P. M. A., Vidaver, W. E., Webster, J. M. and Dube, S. L. 1991. Effect of Bursaphelenchus xylophiIus on the water potential and water-splitting complex of photosystem of Pinus sylvestris seedlings. Physiol. Mol. Plant Pathol. 38:83-91   DOI
19 SYSTAT Software Inc., 2004. SYSTAT 11, Statistics II. SYSTAT Softwarc Inc., Richmond, CA
20 Tan, J. J., Ye, J. R., Wu, X. Q., Zhu, Y. F. and Li, Y. 2005. A study on disease development and early diagnosis of pine wood nematode, Bursaphelenchus xylophilus, infection of Japanese black pine. Nematol. 7:481-485   DOI   ScienceOn
21 Tan, J. J. and Ye, J. R. 2003. Research advance in pathogeuic mechanism of pine wood nematode disease. J. Huazhong Agri. Univ. 22:613-617
22 Woo, K. S., Kim, Y. S., Koo, Y. 8., Yeo, J. K. and Moon, Y. S. 2007. Variation in susceptibility of pine species seedlings with the pinewood nematode, Bursaphelenchus xylophilus, in greenhouse. Plant Pathol. J. 23:70-75   DOI   ScienceOn
23 Woo, K. S., Lee, D. H., Koo, Y. B. and Yeo, J. K. 2008. Inoculation of seven pine species or hybrid seedlings with Korean isolates of pinewood nematode under greenhouse conditions. Ann. For. Sci. 65:811(1-7)   DOI   ScienceOn
24 Tamura, H., Mineo, K. and Yamada, T. 1987. Blockage of water conduction in Pinus thunbergii inoculated with Bursaphelenchus xylophilus. Jpn. J. Nematol. 17:23-30
25 Korea Forest Service. 2007. Statistical yearbook of forestry. No. 37, 485 pp.
26 Kuroda, K. 1991. Mechanism of cavitation development in the pine wilt disease. Eur. J. For. Path. 21:82-89   DOI
27 Futai, K. and Furuno, T. 1979. The variety of resistances among pine-species to pine wood nematode, Bursaphelenchus lignicolus. Bull. Kyoto Univ. For: 51:23-36
28 Futai, K. 2003. Rolc of asymptomatic carrier trees in epidemic spread of pine wilt disease. J. For. Res. 8:253-260   DOI   ScienceOn
29 Kuroda, K. 2004. Inhibiting factors of symptom development in several Japanese red pine (Pinus densijlora) families selected as resistant to pine wilt. J. For. Res. 9:217-224   DOI