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http://dx.doi.org/10.5423/PPJ.2009.25.2.157

Molecular Analysis of Korean Isolate of Barley mild mosaic virus (Iks Isolate)  

Choi, Min-Kyung (Division of Agri-Food and Horticulture, Jeollabuk-Do Agricultural Research and Extension Services)
Kamala-Kannan, Seralathan (Division of Biotechnology, Chonbuk National University)
Oh, Byung-Taek (Division of Biotechnology, Chonbuk National University)
Park, Jong-Chul (Honam Agricultural Research Institute, National Institute of Crop Science, Rural Development Administration)
Lee, Gun-Woong (Division of Biotechnology, Chonbuk National University)
Lee, Kui-Jae (Division of Biotechnology, Chonbuk National University)
Park, Yool-Jin (Department of Environmental Landscape Architecture-Design, Chonbuk National University)
Publication Information
The Plant Pathology Journal / v.25, no.2, 2009 , pp. 157-164 More about this Journal
Abstract
The complete nucleotide sequences of both RNA of an isolated Barley mild mosaic virus (BaMMV) from Iksan, Korea, have been determined. RNA1 was 7273 nucleotides long and encodes for a polyprotein of 2261 amino acids, which contains the eight putative functional proteins. RNA2 was 3520 nucleotides long and encodes for a polyprotein of 894 amino acids, which contains two functional proteins. Results of multiple sequence alignment showed 92.9% similarity with Na1 isolate, followed by Sil, UK(F), Asl1, Remis M and UK(M) isolates, respectively. Comparison of the BaMMV-Iks polyproteins with the corresponding proteins of BaMMV-Na1 isolates showed 95% amino acid sequence identity. The phylogenetic analysis revealed that Iks isolate was closely related to Na1 strain and have a common origin.
Keywords
Barley mild mosaic virus; capsid protein; fungus transmission; Hordeum vulgare; Potyvirus;
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