Browse > Article
http://dx.doi.org/10.5423/PPJ.2009.25.1.021

Mixed Infection of 16S rDNA I and V Groups of Phytoplasma in a Single Jujube Tree  

Lee, Sang-Hun (Department of Plant Medicine, Chungbuk National University)
Han, Sang-Sub (Division of Forest Science, Chonbuk National University)
Cha, Byeong-Jin (Department of Plant Medicine, Chungbuk National University)
Publication Information
The Plant Pathology Journal / v.25, no.1, 2009 , pp. 21-25 More about this Journal
Abstract
Jujube trees infected with phytoplasma exhibit symptoms of typical witches' broom, such as yellowing, abnormally small leaves, short internodes and proliferation of shoots. A 1.2 kb fragment of the 16S rDNA from jujube phytoplasma was generated by R16F2n/R16R2 primer pair from earlier amplified P1/P7 PCR products of cloned jujube witches' broom phytoplasmas. Enzymatic restriction fragment length polymorphism (RFLP) and sequence analysis of 16S rDNA revealed that the jujube tree was infected with 16S rDNA I and V groups of phytoplasmas. Extensive comparative analyses of restriction enzyme profiles from Alu I, Hha I, Msp I, and Rsa I clearly classified the two into different phytoplasma groups. The phylogenie analyses based on 16S rDNA showed that the similarity of the two different clones was 87.5%. This is the first report of a mixed phytoplasmal infection in a single jujube tree.
Keywords
16S rDNA; jujube witches broom; mulberry dwarf phytoplasma; phytoplasma; RFLP;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
1 Alma, A, Davis, R. E., Vibio, M., Danielli, A and Bosco, D.1996. Mixed infection of grapevines in northern Italy by phytoplasma including 16S rRNA RFLP subgroup 1 6SrI-B strains previously unreported in this host. Plant Dis. 80:418-421   DOI
2 Chiykowski, L. N. 1991. Vector-pathogen-host plant relationships of clover phyllody mycoplasmalike organism and the vetor leafhopper Paraphlepsius irroratus. Can. J Plant Pathol. 13:11-18   DOI
3 Kim, Y. H. 1980. Studies on mycoplasma-like organism associated with Witches' broom of Rhus javanica. Korea J Foresry 47:1-15
4 Lee, J. T 1988. Investigation on jujube diseases and their severities of incidence. Res. Rept. RDA (Agri. Institutional cooperation) 31:155-161
5 Lee, J. T and Yea, M. C. 1993. Detection of mycoplasma-like organisms (MLOs) using PCR from diseased plants in Korea. Korea J Mycoplasmol. 4:36-44
6 Shiomi, T. and Sugiura, M. 1984 Difference among Macrosteles orientalis-transmitted MLO, potato purple-top wilt MLO in Japan and aster yellow MLO from USA. Ann. Phytopath. Soc. Japan 50:455-460   DOI
7 Schneider, B., Seemuller, E., Smart, C. D. and Kirkpatrick, B. C. 1995. Phylogenetic classification of plant pathogenic mycoplasmal ike organism or phytoplasmas. In: Molecular and Diagnostic procedures in Mycoplasmology, Vol. 1 ed. by R. Razin and J. G Tully, pp. 369-380. Academic Press, San Diego, USA
8 Staniulis, J. B., Davis, R. E., Jomantiene, R., Kalvelyte, A. and Dally, E. L. 2000. Single and mixed phytoplasma infections in phyllody and dwarf-diseased clover plants in Lithuania. Plant Dis. 84: 1061-1066   DOI   ScienceOn
9 La, Y. J. and Woo, K. S. 1980. Transmission of jujube witches' broom mycoplasma by leafhopper Hishimonas sellatus Uhler. Kor. J For. 48:29-39
10 Liefting, L. W., Padovan, A C., Gibb, K. S., Beever, R. E., Andersen, M. T., Newcomb R. D., Beck, D. L. and Forester, R. L. S. 1998. 'Candidatus phytoplasma austraiense' is the phytoplasma associated with Australian grapevine yellows, papaya dieback and phormium yellow leaf disease. Eur. J Plant Pathol. 104:619-623   DOI   ScienceOn
11 Lee, I.-M., Gundersen., D. E. Hammond, R. W. and Davis, R. E. 1994. Use of mycoplasmalike organism (MLO) group-specific oligonucleotide primer for nested-PCR assays to detect mixed-MLO infections in a single host plant. Phytopathology 84:559-566   DOI   ScienceOn
12 Deng, S. J. and Hiruki, C. 1991. Genetic relatedness between two nonculturable mycoplasmalike organisms revealed by nucleic acid hybridization and polymerase chain reaction. Phytopathology 81:1475-1479   DOI
13 Kim, Y. H., So, I. Y. and Han, S. S. 1994. MLO-DNA nucleotide sequence and diversity of mycoplasmal tree disease in Korea. Korea J Mycoplasmol. 5:53-59
14 Davis, R. E. and Lee, I.-M. 1993. Cluster-specific polymerase chain reaction amplification of 16S rDNA sequences for detection and identification of mycoplasmalike organisms. Phytopathology 83:772-776   DOI   ScienceOn
15 Bianco, P. A, Davis, R. E., Prince, J. P., Lee, I.-M., Gundersen, D. E., F ortusini, A and Belli, G 1993. Double and single infections by aster yellow and elm yellows MLOs in grapevines with symptoms characteristic of flavescence doree. Riv. Patol. Veg. 3:69-82
16 McCoy, R. E., Caudwell, A, Chang, C. J., Chern, T-A, Chiykowski, L. N., Cousin, M. T, Dale, J. L., DeLeeuw, G T. N., Golino, D. A, Hackett, K. J., et al. 1989. Plant disease associated with mycoplasma-like organisms. In: The mycoplasmas, Vol. 5, ed. by R. F. Whitcomb and J. G Tully, pp. 546-640. New York Academic Press, New York, USA
17 Kollar, A, Seemuller, E., Bonet, F., Saillaird, S. and Bove, J. M. 1990. Isolation of the DNA of various plant pathogenic mycoplasmalike organisms from infected plants. Phytopathology 80:3233-3237
18 Han, S. S. and Cha, B. 2002. Genetic similarity between jujube witches' broom and muberry dwarf phytoplasmas transmitted by same vector in korea. Kor. J Plant Pathol. 18:98-101   DOI   ScienceOn
19 McCoy, R. E., Caudwell, A. and Chang, C. J. 1989. Plant diseases associated with mycoplasmalike organisms. pp. 545-560. In: The Mycoplasmas, Vol.5. Academic Press, New York
20 Chiykowski, L. N. and Sinha, P. C. 1988. Some factors affecting the transmission of eastern peach X-mycoplasmalike organism by the leafhopper paraphlepsius irroratus. Can. J Plant Pathol. 10:85-92   DOI
21 Chang, B. H. and Kim, C. J. 1971. studies on the dwarf disease of mulberry tree. Serio J Korea: 17 -21