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http://dx.doi.org/10.5423/PPJ.2008.24.1.036

Virus-like Particles and Cellular Changes in Plants Infected with Sweetpotato Viruses  

Sim, Jeong-Gu (Department of Crop Physiology and Plant Pathology, Louisiana State University)
Valverde, Rodrigo (Department of Crop Physiology and Plant Pathology, Louisiana State University)
Clark, Christopher (Department of Crop Physiology and Plant Pathology, Louisiana State University)
Chun, Se-Chul (Department of Molecular Biotechnology, Konkuk University)
Publication Information
The Plant Pathology Journal / v.24, no.1, 2008 , pp. 36-45 More about this Journal
Abstract
Studies with the transmission electron microscope were used to detect and attempt to identify viruses infecting sweetpotato (Ipomoea batatas) and other Ipomoea species. Flexuous-rods, short curved-rods, and spherical virus-like particles were observed in cells of symptomatic plants. Also, various cytopathic changes such as crystals, vesicles, fibril structures, and cylindrical inclusions were observed. The present study showed that some of these cytopathic changes were associated with some viral groups, which might be helpful in diagnosis.
Keywords
Crinivirus; Cytopathology; Geminivirus; Potyvirus; Sweetpotato viruses;
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1 Winter, S., Purac, A., Leggett, F., Frison, E. A., Rossel, H. W. and Hamilton, R.I. 1992. Partial characterization and molecular cloning of a closterovirus from sweet potato infected with the sweet potato virus disease complex from Nigeria. Phytopathology 82:869-875.   DOI
2 Yamashita, S., Doi, Y. and Shin, K. A. 1984. Ann. Phytopath. Soc. Japan. 50:438.
3 Hollings, M., Stone, M. O. and Bock, K. R. 1976. Purification and properties of sweetpotato mild mottle, a white-fly borne virus from sweetpotato (Ipomoea batatas) in East Africa. Ann. Appl. Biol. 82:511-528.   DOI   ScienceOn
4 Kim, K. S., Gonsalves, D., Teliz, D. and Lee, K. W. 1989. Ultrastructure and mitochondrial vesiculation associated with closteroviruslike particles in leafroll-diseased grapevine. Phytopathology 79:357-360.   DOI
5 Kim, K. S. and Flores, E. M. 1979. Nuclear changes associated with Euphorbia mosaic virus transmitted by the whitefly. Phytopathology 69:980-984.   DOI
6 Clark, C. A. and Moyer, J. W. 1988. Compendium of Sweetpotato Diseases. The American Phytopathological Society. St. Paul, Minnesota. USA.
7 Chavi, F., Robertson, A. I. and Verduin, B. J. M. 1997. Survey and characterization of viruses in sweetpotato from Zimbabwe. Plant Dis. 81:1115-1122.   DOI   ScienceOn
8 Edwardson, J. R. and Christie, R. G. 1996. Cylindrical Inclusions. Bulletin 894. Agricultural Experiment Station. Institute of Food and Agricultural Sciences. University of Florida.
9 Coffin, R. S. and Coutts, R. H. A. 1993. The closteroviruses, capilloviruses and other similar viruses: a short review. J. Gen. Virol. 74:1475-1483.   DOI   ScienceOn
10 Conti, G. G., Vegetti, G., Bassi, M. and Augusta Favali M. 1972. Some ultrastructural and cytochemical observations on Chinese cabbage leaves infected with cauliflower mosaic virus. Virology 47:694-700.   DOI   ScienceOn
11 Pio-Ribeiro, G., Jarret, R. L., Demski, J. W. and Hamilton, R. I. 1996. Detection of sweetpotato virus disease-associated closterovirus in a sweetpotato accession in the United States. Plant Dis. 80:551-554.   DOI
12 Weintraub, M., Ragetli, H. W. J. and Veto, M. 1968. Fine structure of nuclear crystals in leaves of two Dianthus species. Amer. J. Bot. 55:214-220.   DOI   ScienceOn
13 Kim, K. S., Shock, T. L. and Goodman, R. M. 1978. Infection of Phaseolus vulgaris by Bean golden mosaic virus: ultrastructural aspects. Virology 89:22-33.   DOI   ScienceOn
14 Larsen, R. C., Kim, K. S. and Scott, H. A. 1991. Properties and cytopathology of Diodia vein chlorosis virus-a new whiteflytransmitted virus. Phytopathology 81:227-232.   DOI
15 Lastra, R. and Gil, F. 1981. Ultrastructural host cell changes associated with tomato yellow mosaic. Phytopathology 71:524- 528.   DOI
16 Esau, K. and Hoefert, L. L. 1981. Beet yellow stunt virus in the phloem of Sonchus oleraceus. J. Ultrastruct. Res. 75:326-338.   DOI   ScienceOn
17 Lotrakul, P. and Valverde, R. A. 1999. Cloning of a DNA-A-like genome component of sweetpotato leaf curl virus: nucleotide sequence and phylogenetic relationships. Mol. Plant Pathol. On-line publication/1999/0206lotrakul.
18 Lotrakul, P., Valverde, R. A., Clark, C. A., Sim, J. and De La Torre, R. 1998. Detection of a geminivirus infecting sweetpotato in the United States. Plant Dis. 82:1253-1257.   DOI   ScienceOn
19 Francki, R. I. B., Milne, R. G. and Hatta, T. 1985. Atlas of Plant Viruses. CRC Press. Boca Raton. Florida.
20 Gibson, R. W., Mpembe, I., Alicai, T., Carey, E. E., Mwanga, R. O. M., Seal, S. E. and Vetten, H. J. 1997. Symptoms, aetiology and serological analysis of sweetpotato virus disease in Uganda. Pl. Pathol. 47:95-102.
21 Atkey, P. T. and Brunt, A. A. 1987. Electron microscopy of an isometric Caulimo-like virus from sweetpotato (Ipomoea batatas). J. Phytopathology 118:370-376.   DOI
22 Bos, L. 1983. Introduction to Plant Virology. Longman Inc. New York, NY.
23 Ahmed, N. A., Christie, S. R. and Zettler, F. W. 1983. Identification and partial characterization of a Closterovirus infecting Nandina domestica. Phytopathology 73:470-475.   DOI
24 Brunt, A. A., Crabtree, K., Dallwitz, M. J., Gibbs, A. J. and Watson L. 1996. Viruses of Plants, Descriptions and Lists from the VIDE Database. CAB International. University Press, Cambridge, UK.