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http://dx.doi.org/10.5423/PPJ.2007.23.3.151

Phytoplasma-associated Shoot Proliferation and Leaf Yellowing in Lettuce  

Chung, Bong-Nam (National Horticultural Research Institute, Rural Development Administration)
Kim, Jeong-Soo (National Horticultural Research Institute, Rural Development Administration)
Cheong, Seung-Ryong (National Horticultural Research Institute, Rural Development Administration)
Publication Information
The Plant Pathology Journal / v.23, no.3, 2007 , pp. 151-154 More about this Journal
Abstract
Phytoplasma was identified from leaf lettuce (Lactuca sativa) cultivated in commercial green-house in Korea. Diseased leaf lettuce revealed proliferation of shoots, and yellowing and shrinking of leaves (lettuce proliferation-K). Polymerase chain reaction (PCR) with universal primer pair P1/P6, and aster yellows (AY) specific primer pair R16F1/R1 amplified 1.5kb and 1.1kb length of DNA fragments, respectively. Nucleotide sequences of 16S rRNA gene were determined (Gen Bank accession no EF489024). Phylogenetic analysis of 16S rDNA showed the closest relationship with AY phytoplasma (GenBank accession no. AY389822 and AY389826), indicating that lettuce proliferation-K is a member of AY. Phytoplasma bodies were detected in phloem sieve tubes of diseased lettuce by transmission electron microscopy. The structures had round or pleomorphic shapes with a diameter of 130-300nm. Phylogenetic analysis of 16S rRNA gene, microscopic observation of phytoplasma bodies and symptomatology indicated that lettuce proliferation-K is caused by phytoplasma in the AY group. This is the first report of phytoplasma disease in lettuce in Korea.
Keywords
Lactuca sativa; phytoplasma; shoot proliferation; yellowing;
Citations & Related Records
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Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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1 De Vries, I. M. 1997. Origin and domestication of Lactuca sativa L. Genet. Resour. Crop Evol. 44:165-174   DOI   ScienceOn
2 Lee, I. M., Gundersen, D. E., Hammond, R. W. and Davis, R. E. 1994. Use of mycoplasmalike organisms (MLO) group-specific oligonucleotide primers for nested PCR assays to detect mixed MLO infections in a single host plant. Phytopathology 84:559-566   DOI
3 McCoy, R. E., Caudwell, A., Chang, C. J., Chen, T. A., Chiykowski, L. N., Cousin, M. T., Dale, J. L., de Leeuw, G T. N., Golino, D. A., Hackett, K. J., Kirkpatrick, B. C, Marwitz, R., Petzold, H., Sinha, R. C, Sugiura, M., Whitecomb, R. F., Yang, I. L., Zhu, B. M. and Seemiiller, E. 1989. Plant diseases associated with mycoplasmalike organisms. In: The Mycoplasmas vol. 5, ed. by R. F. Whitcomb and J. G Tully, pp.545-640. Academic Press, New York
4 Chung, B. N., Huh, G Y. and Jeong, M. I. 2005. First report on the witches' broom in annual statice (Limonium sinuatum) in Korea. 2005. Plant Pathol. J. 21:383-386   과학기술학회마을   DOI   ScienceOn
5 Beanland, L., Madden, L. V., Hoy, C. W., Miller, S. A. and Nault, L. R. 2005. Temporal distribution of aster leaf hopper sex ratios and spatial pattern of aster yellows phytoplasma disease in Lettuce. Ann. Entomol. Soc. Am. 98:756-762   DOI   ScienceOn
6 Errampalli, D. and Fletcher, J. 1991. Incidence of yellows in carrot and lettuce and characterization of mycoplasmalike organism isolates in Oklahoma. Plant Dis. 75:579-584   DOI
7 Lee, I. M. and Davis, R. E. 1983. Phloem-limited prokaryotes in sieve elements isolated by enzyme treatment of diseased plant tissues. Phytopathology 73: 1540-1543   DOI
8 Olsen, G J., Woese, C. R. and Overbeek, R. 1994. The winds of (evolutionary) change: Breathing new life into microbiology. J. Bacteriol. 176: 1-6   DOI
9 Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Plasmid vectors. In: Molecular Cloning. A Laboratory Manual, 2nd ed., pp.1.38-1.39. Cold Spring Harbor Laboratory Press. Cold Spring Harbor. N.Y
10 Chung, B. N., Choi, G S., Kim, H. R. and Kim, J. S. 2001. Identification of aster yellows phytoplasma in Dendranthema grandiflorum. Plant Pathol. J. 17:57-61
11 Weintraub, P. G and Beanland, L. 2006. Insect vectors of phytoplasmas. Ann. Rev. Entomol. 51:91-111   DOI   ScienceOn
12 Lee, J. T. 2004. Phytoplasmal disease in Korea. Junghaeng-Sa. Korea
13 Lee, I. M., Gundersen, D. E., Davis, R. E. and Bartoszyk, I. M. 1998. Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA and ribosomal protein gene sequences. Int. J. Syst. Bacteriol. 48: 1153-1169   DOI
14 Zhang, J., Hogenhout, S. A., Nault, L. R., Hoy, C. W. and Miller, S. A. 2004. Molecular and symptom analyses of phytoplasma strains from lettuce reveal a diverse population. Phytopathology 94:842-849   DOI   ScienceOn
15 Deng, S. and Hiruki, C. 1991. Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes. J. Microbiol. Methods 14:53-61   DOI   ScienceOn
16 Salehi, M., Izadpanah, K. and Nejat, N. 2006. A new phytoplasma infecting lettuce in Iran. Plant Dis. 90:247