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http://dx.doi.org/10.5423/PPJ.2007.23.2.045

Genetic Variability of Sorghum Charcoal Rot Pathogen (Macrophomina phaseolina) Assessed by Random DNA Markers  

Bashasab, Rajkumar, Fakrudin (Institute of Agri-Biotechnology, University of Agricultural Sciences)
Kuruvinashetti, Mahaling S (Institute of Agri-Biotechnology, University of Agricultural Sciences)
Publication Information
The Plant Pathology Journal / v.23, no.2, 2007 , pp. 45-50 More about this Journal
Abstract
Genetic diversity among selected isolates of Macrophomina phaseolina, a causal agent of charcoal rot (stalk rot) disease in sorghum was studied using PCR-RAPD markers. A set of ten isolates, from ten different rabi sorghum genotypes representing two traditional sorghum growing situations viz., Dharwad- a transitional high rainfall region and Bijapur- a semi-arid low rainfall region in South India. From a set of 40 random primers tested, amplicon profiles of 15 were reproducible. A total of 149 amplicon levels, with an average of 9.9 bands per primer, were available for analysis, of which 148 were polymorphic (99.3%). It was possible to discriminate all the isolates with any of the 15 primers employed. UPGMA clustering of data indicated that the isolates shared varied levels of genetic similarity within a range of 0.14 to 0.72 similarity coefficient index and it was suggestive that grouping of isolates was not related to sampling location in anyway. A high level of genetic heterogeneity of 0.28 was recorded among the isolates.
Keywords
sorghum; charcoal rot; Macrophomina phaseolina; genetic diversity; PCR-RAPD marker;
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