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http://dx.doi.org/10.5423/PPJ.2006.22.2.152

Effect of temperature on the Concentration of Chrysanthemum stunt viroid in CSVd-infected Chrysanthemum  

Chung, Bong-Nam (National Horticultural Research institute, Rural Development Administration)
Huh, Eun-Joo (National Horticultural Research institute, Rural Development Administration)
Kim, Jeong-Soo (National Horticultural Research institute, Rural Development Administration)
Publication Information
The Plant Pathology Journal / v.22, no.2, 2006 , pp. 152-154 More about this Journal
Abstract
CSVd-infected chrysanthemum plants grown under $10^{\circ}C\;or\;15^{\circ}C$ growth chamber for 2 months resulted a higher dilution endpoint of template RNA for Reverse transcription and polymerase chain reaction (RT-PCR) than those grown for 1 month: $10^{-4}(1.35{\times}10^{-2}ug/ml)$ for 1 month, and $10^{-3}(1.35{\times}10^{-1}ug/ml)$ for 2 months. Independent experiment, shoots cut from CSVd (Chrysanthemum stunt viroid)-in footed chrysanthemum plants grown under $10^{\circ}C\;or\;20^{\circ}C$ growth chamber for 2 months showed the same CSVd concentration as control($30^{\circ}C$) at 8 weeks after moving them to normal green-house condition($30^{\circ}C$). From those results, it was concluded that even though the concentration of CSVd was reduced in plants grown at low temperatures, when they were moved to normal glass-house temperature CSVd concentration increased to that of untreated plants in 8 weeks. This conclusion was supported by the rapid replication of CSVd in chrysanthemum after infection.
Keywords
Chrysanthemum; CSVd; low temperature;
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Times Cited By KSCI : 1  (Citation Analysis)
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